Untitled

Козловский, А. Г.; Желифонова, В. П.; Adanin, V. M.; Антипова, Т. В.; Shnyreva, A.V.; Viktorov, A. N.

doi:10.1023/a:1021475722091

Cited by 14 publications

(10 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was shown earlier that the growth of P. citrinum strain VKM FW-800 in the control medium was characterized by the absence of marked diauxy, despite the presence of two sources of carbon (succinate and mannitol) in the culture medium [2]. However, the growth of this fungus in the zinc-containing medium is characterized by diauxy and two µ max values (Fig.…”

Section: Resultsmentioning

confidence: 74%

“…It was shown earlier that the addition of zinc ions to the control medium stimulates both the basal metabolic processes (greater amounts of the biomass are produced in the presence of zinc compared to the control medium) and the synthesis of clavine alkaloids in P. citrinum strain VKM FW-800 [2]. The addition of zinc ions to the control medium also stimulated the synthesis of quinocitrinines (Tables 1, 2): the concentration of quinocitrinines in culture liquid increased 16 times; the yield of quinocitrinines per biomass unit ( Y ïÄ/ï ) increased approximately 8 times; and the maximal rate of synthesis of quinocitrinines ( q ïÄ ) increased approximately 6 times as compared to the control medium.…”

Section: Resultsmentioning

confidence: 99%

“…For this reason, relic fungi isolated from permafrost sediments are of special interest. It was discovered earlier that Penicillium citrinum strain VKM FW-800, isolated from Arctic permafrost sediments, synthesizes agroclavine I and epoxyagroclavine I-rare clavine ergot alkaloids with the 5 R ,10 S configuration of the ergoline nucleus [2]. Additionally, novel quinoline alkaloids quinocitrinines, quinocitrinine A and quinocitrinine B exhibiting biological activity (including antibiotic properties) were isolated from the culture liquid of this fungus [3].…”

mentioning

confidence: 95%

“…To study the time course of culture growth and synthesis of alkaloids, samples were taken in triplicate at 24-h intervals. The growth of the fungus was assessed by changes in dry mycelium weight during culturing.Metabolites were extracted from culture-liquid filtrate and from vegetative mycelium as described earlier [2,3] and analyzed by TLC on silica gel plates (Silica gel 60 F 254 , Merck, Germany) in the following chloroform-methanol-25% NH 4 OH (conc.) systems differing in the proportion of components: (90 : 10 : 0.1; system I), (90 : 10 : 1; system II), and (80 : 20 : 0.2; system III).…”

mentioning

confidence: 99%

See 3 more Smart Citations

The Fungus Penicillium citrinum, Isolated from Permafrost Sediments, as a Producer of Ergot Alkaloids and New Quinoline Alkaloids Quinocitrinines

Kozlovsky

Желифонова

Антипова

2005

Appl Biochem Microbiol

Self Cite

View full text Add to dashboard Cite

Fungi of the genus Penicillium are promising objects in terms of searching for new biologically active compounds. Approximately 380 secondary metabolites produced by these fungi are known, some of which are biologically active [1]. Today, the search for new producers of biologically active compounds is actively underway among fungi growing under extreme conditions, because the synthesis of new secondary metabolites and potential biologically active compounds that help them to survive and adapt to these conditions can be expected in these fungi with the greatest probability. For this reason, relic fungi isolated from permafrost sediments are of special interest. It was discovered earlier that Penicillium citrinum strain VKM FW-800, isolated from Arctic permafrost sediments, synthesizes agroclavine I and epoxyagroclavine I-rare clavine ergot alkaloids with the 5 R ,10 S configuration of the ergoline nucleus [2]. Additionally, novel quinoline alkaloids quinocitrinines, quinocitrinine A and quinocitrinine B exhibiting biological activity (including antibiotic properties) were isolated from the culture liquid of this fungus [3].The twofold goal of this work was to study (1) the biosynthesis of ergot alkaloids and quinocitrinines by P. citrinum strain VKM FW-800 during culture growth and (2) the effect of zinc ions on the main parameters of growth and alkaloid production. MATERIALS AND METHODSThis study was performed with the P. citrinum strain VKM FW-800, isolated from 1.8-to 3-million-year-old Arctic permafrost sediments [4], which was maintained on glucose-potato slanting agar. Fourteen-day-old culture ( 1-2 × 10 7 spores/ml) was used as an inoculum. The fungus was cultured in 750-ml Erlenmeyer flasks in 150 ml of the control medium at 24 ± 1°ë on a shaker (220 rpm). The control culture medium was prepared in distilled water and contained 50.0 g/l mannitol, 5.4 g/l succinic acid, 0.3 g/l MgSO 4 · 7 H 2 O, and 1.0 g/l äç 2 PO 4 (pH was adjusted to 5.4 with concentrated ammonia). To study the effect of zinc ions on culture growth and alkaloid production, ZnSO 4 · 7 H 2 O was added to the control medium to the final concentration of 1 mg/l. To study the time course of culture growth and synthesis of alkaloids, samples were taken in triplicate at 24-h intervals. The growth of the fungus was assessed by changes in dry mycelium weight during culturing.Metabolites were extracted from culture-liquid filtrate and from vegetative mycelium as described earlier [2,3] and analyzed by TLC on silica gel plates (Silica gel 60 F 254 , Merck, Germany) in the following chloroform-methanol-25% NH 4 OH (conc.) systems differing in the proportion of components: (90 : 10 : 0.1; system I), (90 : 10 : 1; system II), and (80 : 20 : 0.2; system III). Compounds of interest were detected by absorption in ultraviolet light after spraying the plates with the Ehrlich or Dragendorff reagents (for identification of indole compounds and nitrogen-containing metabolites, respectively).The total amount of ergot alkaloids in the specimens was determined ...

show abstract

Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 95%

mentioning

confidence: 99%

See 2 more Smart Citations

The Fungus Penicillium citrinum, Isolated from Permafrost Sediments, as a Producer of Ergot Alkaloids and New Quinoline Alkaloids Quinocitrinines

Kozlovsky

Желифонова

Антипова

2005

Appl Biochem Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…For the isolation of viridicatol, see: Yurchenko et al (2010); Fremlin et al (2009) ;Proksch et al (2008); Lund & Frisvad (1994); Birkinshaw et al (1963); Kozlovskii et al (2002). For the synthesis of viridicatol, see: Kobayashi & Harayama (2009).…”

Section: Related Literaturementioning

confidence: 99%

3-Hydroxy-4-(3-hydroxyphenyl)-2-quinolone monohydrate

Tao

Wang

2011

Acta Cryst E

View full text Add to dashboard Cite

In the title compound, also known as viridicatol monohydrate, C15H11NO3·H2O, the dihedral angle between the benzene ring and quinoline ring system is 64.76 (5)°. An intramolecular O—H⋯O hydrogen bond occurs. The crystal structure is stabilized by classical intermolecular N—H⋯O and O—H⋯O hydrogen bonds and weak π–π interactions with a centroid–centroid distance of 3.8158 (10) Å.

show abstract