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Wada, Masato; Cao, Qiufen; Kotoda, Nobuhiro; Soejima, Junichi; Masuda, Takeshi

doi:10.1023/a:1015544207121

Cited by 134 publications

(19 citation statements)

References 26 publications

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“…It was shown that over-expression of AFL1 or AFL2 , the LFY homologs in apple, can induce the early-flowering in Arabidopsis (Wada et al, 2002). In other reports, over-expression of AtLFY in apple didn’t show the same results instead of a columnar phenotype with shorten internodes (Flachowsky et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Over-expression of EjLFY-1 Leads to an Early Flowering Habit in Strawberry (Fragaria × ananassa) and Its Asexual Progeny

et al. 2017

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As a master regulator involved in flower development, LEAFY-like gene has been demonstrated to play a key role in the flowering process regulation of angiosperms. Expression analysis of EjLFY-1, a LEAFY (LFY) homolog of loquat (Eriobotrya japonica Lindl.), indicated its participation in the regulation of flowering in loquat. To verify its function and potential value in the genetic engineering to shorten the juvenile phase, ectopic expression of EjLFY-1 in strawberry (Fragaria × ananassa) was achieved using Agrobacterium-mediated gene transfer of a plant expression vector with the loquat EjLFY-1 gene driven by the CaMV 35S promoter. Totally 59 plantlets were verified to be the transformants. The presence, expression and integration of EjLFY-1 in the transformants were assessed by PCR, quantitative real-time PCR and Southern blot, respectively. Constitutive expression of EjLFY-1 in strawberry accelerated the flowering process in strawberry with the shorten necessary period for flowering induction, development of flower and fruit set. While vegetative growth habits of the transformants in the first cropping season were consistent with the WT ones. Meanwhile, both the flowers and fruits of the transformants were also as same as those of the WT ones. Furthermore, the early-flowering habit was maintained in their asexual progeny, the runner plants. While with continuous asexual propagation, the clones showed a more strengthen early-flowering phenotype, such as the reduced vegetative growth and the abnormal floral organs in individual plantlets. These results demonstrated the function of this gene and at the same time provided us new insights into the utilization potential of such genes in the genetic engineering of perennial fruits.

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Section: Discussionmentioning

confidence: 99%

Over-expression of EjLFY-1 Leads to an Early Flowering Habit in Strawberry (Fragaria × ananassa) and Its Asexual Progeny

et al. 2017

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“…Such genes are isolated from flower buds in the still initial phases of their development, from flower organs and fruits of some apple cultivars (68,110,111,118,129).…”

Section: Development Of the Flower Budsmentioning

confidence: 99%

Flower Induction and Flower Bud Development in Apple and Sweet Cherry

Koutinas

Pepelyankov

Lichev

2010

Biotechnology & Biotechnological Equipment

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“…Although there are fundamental differences in the flowering process between annual and perennial plants, the genetics of FI and floral organ formation are likely to be similar among these plants (Tan and Swain, 2006). A set of apple genes with sequence similarity to genes involved in floral meristem transition of Arabidopsis has been identified and subjected to expression studies (Jeong et al , 1999; Sung et al , 1999; Kotoda et al , 2000, 2002, 2003, 2006, 2010; Van der Linden et al , 2002; Wada et al , 2002; Kotoda and Wada, 2005; Esumi et al , 2005). Overexpression of the apple gene orthologues of LFY , AFL1 , and AFL2 ( APPLE FLORICAULA/LFY ) (Wada et al , 2002), as well as MdMADS2 and MdMADS5 , orthologues of the Arabidopsis FRUITFULL ( FUL ) and AP1 , resulted in early flowering in heterologous systems (Sung et al , 1999; Kotoda et al , 2002).…”

Section: Introductionmentioning

confidence: 99%

Genetic control of biennial bearing in apple

Guitton

Kelner

Velasco

et al. 2011

Journal of Experimental Botany

151

116

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Although flowering in mature fruit trees is recurrent, floral induction can be strongly inhibited by concurrent fruiting, leading to a pattern of irregular fruiting across consecutive years referred to as biennial bearing. The genetic determinants of biennial bearing in apple were investigated using the 114 flowering individuals from an F1 population of 122 genotypes, from a ‘Starkrimson’ (strong biennial bearer)×‘Granny Smith’ (regular bearer) cross. The number of inflorescences, and the number and the mass of harvested fruit were recorded over 6 years and used to calculate 26 variables and indices quantifying yield, precocity of production, and biennial bearing. Inflorescence traits exhibited the highest genotypic effect, and three quantitative trait loci (QTLs) on linkage group (LG) 4, LG8, and LG10 explained 50% of the phenotypic variability for biennial bearing. Apple orthologues of flowering and hormone-related genes were retrieved from the whole-genome assembly of ‘Golden Delicious’ and their position was compared with QTLs. Four main genomic regions that contain floral integrator genes, meristem identity genes, and gibberellin oxidase genes co-located with QTLs. The results indicated that flowering genes are less likely to be responsible for biennial bearing than hormone-related genes. New hypotheses for the control of biennial bearing emerged from QTL and candidate gene co-locations and suggest the involvement of different physiological processes such as the regulation of flowering genes by hormones. The correlation between tree architecture and biennial bearing is also discussed.

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Untitled

Cited by 134 publications

References 26 publications

Over-expression of EjLFY-1 Leads to an Early Flowering Habit in Strawberry (Fragaria × ananassa) and Its Asexual Progeny

Over-expression of EjLFY-1 Leads to an Early Flowering Habit in Strawberry (Fragaria × ananassa) and Its Asexual Progeny

Flower Induction and Flower Bud Development in Apple and Sweet Cherry

Genetic control of biennial bearing in apple

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