XAFS spectroscopy of a single neuron from a patient with Parkinson's disease

Yoshida, Sohei; Ektessabi, A. M.; Fujisawa, Shigeyoshi

doi:10.1107/s0909049500017726

Cited by 50 publications

(35 citation statements)

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“…The microscopic XANES (micro-XANES) technique was applied for investigations on PD as well [34][35][36]. Yoshida et al [34] [34]. In another study, Yoshida et al [36] investigated the differences in Fe oxidation state in the area of neurons, free-NM aggregates and glial cells from PD and control SN.…”

Section: Introductionmentioning

confidence: 99%

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Investigations of differences in iron oxidation state inside single neurons from substantia nigra of Parkinson’s disease and control patients using the micro-XANES technique

Chwiej

Adamek

Szczerbowska-Boruchowska

et al. 2006

J Biol Inorg Chem

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X-ray absorption near edge structure spectroscopy was applied in order to investigate differences in iron chemical state between the nerve cells of substantia nigra (SN) representing Parkinson's disease (PD) and those of control cases. Autopsy samples were cut using a cryotome, and were not fixed and not embedded in paraffin. The comparison of the absorption spectra near the iron K-edge measured in melanized neurons from SN of PD and control samples did not show significant differences in iron oxidation state. Measurements of inorganic reference materials containing iron in the second and third oxidation states indicate that most of the iron in all the nerve cell bodies examined was oxidized and occurred as trivalent ferric iron (Fe 3+ ).

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Section: Introductionmentioning

confidence: 99%

“…The microscopic XANES (micro-XANES) technique was applied for investigations on PD as well [34][35][36]. Yoshida et al [34] [34].…”

Section: Introductionmentioning

confidence: 99%

Investigations of differences in iron oxidation state inside single neurons from substantia nigra of Parkinson’s disease and control patients using the micro-XANES technique

Chwiej

Adamek

Szczerbowska-Boruchowska

et al. 2006

J Biol Inorg Chem

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“…This finding is in line with previous investigations done by several research groups related to iron concentration in neuromelanin from PD cases. [24][25][26] XRF imaging directly revealed the locations of iron inside the neuromelanin granules of the pathological tissue (Fig. 4).…”

Section: Possible Role Of Oxidative Stress On Neurodegenerationmentioning

confidence: 99%

The Role of Trace Metallic Elements in Neurodegenerative Disorders: Quantitative Analysis Using XRF and XANES Spectroscopy

Ide‐Ektessabi

Rabionet

2005

ANAL. SCI.

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Metallic elements are required in numerous essential biological processes of the human body. Transition metal elements like iron, zinc or copper bound to proteins (metalloproteins) perform a variety of complex biological functions as catalysts which regulate biochemical reactions and physiological functions in cells and organs. Published reports have been pointing out that excessive accumulation or a disequilibrium within the concentrations or distributions of these elements may affect cell functions up to the point of degeneration or cell death. 1 Specifically, imbalances of trace metallic elements may be deeply related to neuronal cell death in several neurodegenerative disorders by promoting oxidative stress. 2,3The aim of our studies have been the investigation of the distribution, concentration and chemical state of trace metallic elements in the cells, in order to understand the role of these elements in neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease and Amyotrophic lateral sclerosis. To gain a broader understanding of the underlying behavior of these elements in the neurodegeneration process, samples derived from human, monkey and mouse specimens were studied.For carrying out the analyses of these experiments, X-ray fluorescence spectroscopy (XRF) and X-ray absorption near edge structure spectroscopy (XANES), both using synchrotron radiation (SR) microbeams were applied. Synchrotron radiation is an ideal X-ray source which provides continuous and high intensity photon energy. Wide energy tunability and high brightness and collimation are the main features of SR. This allows the investigation of trace metallic elements with a low detection limit (∼0.1 pg) and high spatial resolution. SR-XRF is used for identifying trace elements, as well as determining their distribution and concentration on a single cell level. On the other side, SR-XANES is sensitive to valence state, local atomic structure and chemical state of the elements of the samples. Futhermore, the non-invasive nature of XRF and XANES techniques allows further examinations of the biological samples a posteriori by complementary methods, e.g. histological observations by staining, rendering procedures like isolating or purifying, basically unnecessary. Excessive Accumulations of Fe, Zn and Cu in the Neurons from an Alzheimer's Disease Patient IntroductionAlzheimer's disease (AD) is a progressive neurodegenerative disorder which gradually affects memory, thinking, communication, emotion and behavior. The pathological features of AD are senile plaques, neurofibrillary tangles, neuropil thread formation and neuron and synapse loss. The cause of this dementia is still not clear but recent investigations suggested that the deposition of amyloid-β (Aβ) peptide, free radical mediated processes and genetic defects like mutations of presenilin contribute to the pathogenesis of AD. These molecules combined with excessive accumulation of metallic elements or possible changes in the chemical state of transition metals like iron, c...

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“…Moreover, the micro-SRXRF was previously satisfactory applied to elemental analysis of cultured single cells as well as thin tissue slices, including neurological applications [16][17][18][19]. Yoshida et al [20] studied the distribution and chemical state of iron in single neurons from substantia nigra (SN) of Parkinson's patient. However, the applied sample pretreatment i.e.…”

Section: Introductionmentioning

confidence: 99%

Implementation of X-ray Fluorescence Microscopy for Investigation of Elemental Abnormalities in Amyotrophic Lateral Sclerosis

Tomik

Chwiej

Szczerbowska-Boruchowska

et al. 2006

Neurochem Res

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The abnormalities of metallochemical reactions may contribute to the pathogenesis of Amyotrophic Lateral Sclerosis (ALS). In the present work, an investigation of the elemental composition of the gray matter, nerve cells and white matter from spinal cord tissues representing three ALS cases and five non-ALS controls was performed. This was done with the use of the synchrotron microbeam X-ray fluorescence technique (micro-SRXRF). The following elements were detected in the tissue sections: P, S, Cl, K, Ca, Fe, Cu, Zn and Br. A higher accumulation of Cl, K, Ca, Zn and Br was observed in the nerve cell bodies than in the surrounding tissue. Contrary to all other elements, Zn accumulation was lower in the white matter areas than in the gray matter ones. The results of quantitative analysis showed that there were no general abnormalities in the elemental accumulation between the ALS and the control group. However, for individual ALS cases such abnormalities were observed for the nerve cells. We also demonstrated differences in the elemental accumulation between the analyzed ALS cases.

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XAFS spectroscopy of a single neuron from a patient with Parkinson's disease

Cited by 50 publications

References 3 publications

Investigations of differences in iron oxidation state inside single neurons from substantia nigra of Parkinson’s disease and control patients using the micro-XANES technique

Investigations of differences in iron oxidation state inside single neurons from substantia nigra of Parkinson’s disease and control patients using the micro-XANES technique

The Role of Trace Metallic Elements in Neurodegenerative Disorders: Quantitative Analysis Using XRF and XANES Spectroscopy

Implementation of X-ray Fluorescence Microscopy for Investigation of Elemental Abnormalities in Amyotrophic Lateral Sclerosis

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