Xanthine derivatives inhibit the increase in intracellular Ca2+ concentration induced by acetylcholine in nasal gland acinar cells of the guinea-pig

Abstract

Xanthine derivatives are also known to have inhibitory effects [8][9][10][11][12]. In the present paper, we provide confirmatory evidence that xanthines directly inhibit both Ca 2+ mobilization and entry induced by acetylcholine (ACh) in the nasal gland acinar cells. Material and methods Preparation of isolaled acini from submucosal nasal glandHealthy albino guinea-pigs weighing 200-300 g were anaesthetized by inhalation of diethyl ether. After decapitation, the nasal septum was quickly removed and placed in an oxygenated cell-storage solution. The cell-storage solution was prepared by the addition of 0.2% bovine serum albumin (BSA) to a standard solution composed of (in mM) 139.