Xanthine:NAD+ oxidoreductase from embryo liver of hen Gallus gallus

Zakrzewska, Barbara; Jeżewska, Maria

doi:10.1016/0305-0491(90)90192-v

Cited by 2 publications

(2 citation statements)

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“…In contrast to mammals, in poultry, the liver is the most important organ responsible for energy metabolism and de novo fatty acid synthesis. , In the liver, adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) functions as both a “regulator” and “sensor” of energy and lipid metabolism. , Therefore, in the current study, we aimed to evaluate the effect of (−)-HCA on factors related to lipid and energy metabolism, and further determine whether this action of (−)-HCA is related to the activation of AMPK in the liver of broiler chickens. The results will reveal the detailed molecular mechanism of glycolipid metabolism regulation by (−)-HCA and will reinforce its potential as a nutritional additive to manage obesity-related diseases in broiler chickens.…”

Section: Introductionmentioning

confidence: 99%

(−)-Hydroxycitric Acid Suppresses Lipid Droplet Accumulation and Accelerates Energy Metabolism via Activation of the Adiponectin-AMPK Signaling Pathway in Broiler Chickens

Zhang

Yao

et al. 2019

J. Agric. Food Chem.

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(−)-Hydroxycitric acid (HCA) inhibits the deposition of fat in animals and humans, while the molecular mechanism is still unclear. The present study investigated the effect and mechanism of (−)-HCA’s regulation of lipid, glucose, and energy metabolism in broiler chickens. The current results showed that (−)-HCA decreased the accumulation of lipid droplets and triglyceride content by reducing fatty acid synthase protein level and enhancing phosphorylation of acetyl-CoA carboxylase protein level. (−)-HCA accelerated carbohydrate aerobic metabolisms by increasing the activities of phosphofructokinase-1, pyruvate dehydrogenase, succinate dehydrogenase, and malate dehydrogenase. Furthermore, (−)-HCA increased adiponectin receptor 1 mRNA level and enhanced phospho-AMPKα, peroxisome proliferator-activated receptor gamma coactivator-1α, nuclear respiratory factor-1, and mitochondrial transcription factor A protein levels in broiler chickens. These data indicated that (−)-HCA reduced lipid droplet accumulation, improved glucose catabolism, and accelerated energy metabolism in broiler chickens, possibly via activation of adiponectin-AMPK signaling pathway. These results revealed the biochemical mechanism of (−)-HCA-mediated fat accumulation and the prevention of metabolic disorder-related diseases in broiler chickens.

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Section: Introductionmentioning

confidence: 99%

(−)-Hydroxycitric Acid Suppresses Lipid Droplet Accumulation and Accelerates Energy Metabolism via Activation of the Adiponectin-AMPK Signaling Pathway in Broiler Chickens

Zhang

Yao

et al. 2019

J. Agric. Food Chem.

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“…Unlike mammalian species, the liver is the most important organ of de novo fatty acid synthesis [35, 40] and energy metabolism [41] in poultry. Therefore, the present study was conducted to investigate the effects of the (-)-HCA on lipid metabolism and energy metabolism in cultured primary chicken hepatocytes, which will provide useful information for further understanding of the biochemical mechanisms associated with the fat accumulation regulation by (-)-HCA in broiler chicken.…”

Section: Introductionmentioning

confidence: 99%

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

Li¹,

Peng²,

Ge³

et al. 2017

Cell Physiol Biochem

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Background/Aims: (-)-Hydroxycitric acid (HCA) had been shown to suppress fat accumulation in animals and humans, while the underlying biochemical mechanism is not fully understood, especially little information is available on whether (-)-HCA regulates energy metabolism and consequently affects fat deposition. Methods: Hepatocytes were cultured for 24 h and then exposed to (-)-HCA (0, 1, 10, 50 µM), enzyme protein content was determined by ELISA; lipid metabolism gene mRNA levels were detected by RT-PCR. Results: (-)-HCA significantly decreased the number and total area of lipid droplets. ATP-citrate lyase, fatty acid synthase and sterol regulatory element binding protein-1c mRNA level were significantly decreased after (-)-HCA treatment, whereas peroxisome proliferator-activated receptor α mRNA level was significantly increased. (-)-HCA significantly decreased ATP-citrate lyase activity and acetyl-CoA content in cytosol, but significantly increased glucose consumption and mitochondrial oxygen consumption rate. (-)-HCA promoted the activity/content of glucokinase, phosphofructokinase-1, pyruvate kinase, pyruvate dehydrogenase, citrate synthase, aconitase, succinate dehydrogenase, malate dehydrogenase, NADH dehydrogenase and ATP synthase remarkably. Conclusions: (-)-HCA decreased lipid droplets accumulation by reducing acetyl-CoA supply, which mainly achieved via inhibition of ATP-citrate lyase, and accelerating energy metabolism in chicken hepatocytes. These results proposed a biochemical mechanism of fat reduction by (-)-HCA in broiler chickens in term of energy metabolism.

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Xanthine:NAD+ oxidoreductase from embryo liver of hen Gallus gallus

Cited by 2 publications

References 3 publications

(−)-Hydroxycitric Acid Suppresses Lipid Droplet Accumulation and Accelerates Energy Metabolism via Activation of the Adiponectin-AMPK Signaling Pathway in Broiler Chickens

(−)-Hydroxycitric Acid Suppresses Lipid Droplet Accumulation and Accelerates Energy Metabolism via Activation of the Adiponectin-AMPK Signaling Pathway in Broiler Chickens

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

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