Xanthomonas arboricola pv. fragariae: a confirmation of the pathogenicity of the pathotype strain

Ferrante, Patrizia; Scortichini, M.

doi:10.1007/s10658-017-1326-2

Cited by 9 publications

(15 citation statements)

References 10 publications

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“…However, the lack of most Hrp T3SS proteins in LMG 19145 PT , CFBP 6773 and LMG 19144 strains could not explain its non-pathogenic behavior since the strain LMG 19146 harboring the full Hrp cluster was not pathogenic either. A detailed study of the pathogenicity of this pathovar and its host range needs thus to be done, as strain LMG 19145 PT was recently confirmed as causing symptoms on strawberry plants from cultivars Candonga, Sabrina, and Murano (Ferrante and Scortichini 2018) suggesting that another virulence factor may cause symptoms on some strawberry cultivars. In this study, the same strain, inoculated on the cultivar Elsanta, did not produce any symptoms.…”

Section: Discussionmentioning

confidence: 99%

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Comparative genomics of Xanthomonas fragariae and Xanthomonas arboricola pv. fragariae reveals intra- and interspecies variations

et al. 2020

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The quarantine bacterium Xanthomonas fragariae causes angular leaf spots on strawberry. Its population structure was recently found to be divided into four (sub)groups resulting from two distinct main groups. Xanthomonas arboricola pv. fragariae causes bacterial leaf blight, but the bacterium has an unclear virulence status on strawberry. In this study, we use comparative genomics to provide an overview of the genomic variations of a set of 58 X. fragariae and five X. arboricola pv. fragariae genomes with a focus on virulence-related proteins. Structural differences within X. fragariae such as differential plasmid presence and large-scale genomic rearrangements were observed. On the other hand, the virulence-related protein repertoire was found to vary greatly at the interspecies level. In three out of five sequenced X. arboricola pv. fragariae strains, the major part of the Hrp type III secretion system was lacking. An inoculation test with strains from all four X. fragariae (sub)groups and X. arboricola pv. fragariae resulted in an interspecies difference in symptom induction since no symptoms were observed on the plants inoculated with X. arboricola pv. fragariae. Our analysis suggests that all X. fragariae (sub)groups are pathogenic on strawberry plants. On the other hand, the first genomic investigations of X. arboricola pv. fragariae revealed a potential lack of certain key virulence-related factors which may be related to the difficulties to reproduce symptoms on strawberry and could question the plant-host interaction of the pathovar.

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Section: Discussionmentioning

confidence: 99%

“…In contrast to the water-soaked regions on the leaves resulting from X. fragariae, the symptoms caused by Xanthomonas arboricola pv. fragariae were reddishbrown lesions on the leaf surface that enlarge and become surrounded by a chlorotic halo (Ferrante and Scortichini 2018). X. arboricola pv.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics of Xanthomonas fragariae and Xanthomonas arboricola pv. fragariae reveals intra- and interspecies variations

et al. 2020

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“…fragariae strains, as observed in previous studies ( 4 , 5 , 9 – 11 ). Very recently, the pathogenicity of the pathotype strain was proved to produce typical symptoms in the strawberry cultivars Candonga, Sabrina, and Murano ( 12 ).…”

Section: Genome Announcementmentioning

confidence: 99%

High-Quality Draft Genome Sequences of Five Xanthomonas arboricola pv. fragariae Isolates

et al. 2018

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“…Additionally, similar inoculation methods, which were found in our case to be best for inoculation, such as high-pressure spraying on both sides of leaves with a bacterial suspension in sterile water, were used for pathogenicity tests that were performed to confirm the pathogenicity of the pathotype strain of Xanthomonas arboricola pv. fragariae (another pathogen of strawberry [10]). In they work only slight differences in symptoms development were observed for inoculations using low-pressure spraying of the bacterial suspension (10 7 cfu mL −1 ) on the lower surface of leaves [10].…”

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confidence: 99%

“…fragariae (another pathogen of strawberry [10]). In they work only slight differences in symptoms development were observed for inoculations using low-pressure spraying of the bacterial suspension (10 7 cfu mL −1 ) on the lower surface of leaves [10].…”

mentioning

confidence: 99%

mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression

2019

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This is the first study which describes a unique procedure of isolating of high-quality, intact RNA from strawberry leaves of Xanthomonas fragariae, three most suitable reference genes, crucial for the normalization of RT-qPCR data for this pathogen and accurate expression analysis of target genes. In our study, various mathematic algorithms: NormFinder geNorm, Best-Keeper, the delta CT method, RefFinder were adopted for validation of most stable reference genes from nine candidate genes (ffh, glyA, gyrA, gyrB, proC, pykA, recA, rpoB, rpoD). The analyses allowing to select three most suitable pioneer reference genes, gyrB, ffh, and pykA, that we recommend for the normalization of RT-qPCR data and for the study of the expression of target genes in Xf. Moreover, their combination as references allowed for an accurate expression analysis and computation of the fold change of the flhF and iroN2 genes in Xf. These two genes are important for the success of the colonization of plant tissue and pathogenicity and sequences of primers designed to study these genes, are presented.

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Xanthomonas arboricola pv. fragariae: a confirmation of the pathogenicity of the pathotype strain

Cited by 9 publications

References 10 publications

Comparative genomics of Xanthomonas fragariae and Xanthomonas arboricola pv. fragariae reveals intra- and interspecies variations

Comparative genomics of Xanthomonas fragariae and Xanthomonas arboricola pv. fragariae reveals intra- and interspecies variations

High-Quality Draft Genome Sequences of Five Xanthomonas arboricola pv. fragariae Isolates

mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression

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