2006
DOI: 10.1016/j.steroids.2005.10.002
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Xanthomonas maltophilia CBS 897.97 as a source of new 7β- and 7α-hydroxysteroid dehydrogenases and cholylglycine hydrolase: Improved biotransformations of bile acids

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Cited by 45 publications
(37 citation statements)
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“…Few enzymes (acylases, EC: 3.5.1) have been reported to hydrolyse glycinates and taurinates to the corresponding carboxylic acid. Recently, Pedrini et al [ 33 ] have isolated and characterized a cholylglycine hydrolase from Xanthomonas maltophilia CBS 827.97: this enzyme completely hydrolyses glycine and taurine conjugates in 20 minutes at 50 °C. Unfortunately the protein sequence of this enzyme is not reported, making recombinant expression and its industrial use impossible.…”
Section: Reviewmentioning
confidence: 99%
“…Few enzymes (acylases, EC: 3.5.1) have been reported to hydrolyse glycinates and taurinates to the corresponding carboxylic acid. Recently, Pedrini et al [ 33 ] have isolated and characterized a cholylglycine hydrolase from Xanthomonas maltophilia CBS 827.97: this enzyme completely hydrolyses glycine and taurine conjugates in 20 minutes at 50 °C. Unfortunately the protein sequence of this enzyme is not reported, making recombinant expression and its industrial use impossible.…”
Section: Reviewmentioning
confidence: 99%
“…Indeed the UDCA 3-azides were more toxic than the corresponding DCA compounds. The 7b-azido compound (34) was also cytotoxic (CC 50 99 lM) however the UDCA-based 24-azide had not effect on cell viability at 500 lM despite its high lipophilicity; (iv) the DCA and UDCA primary amides (25,14) markedly reduced viability (CC 50 39, 161 lM, respectively); (v) in contrast, the DCA methyl ester (24) was more toxic that DCA itself but the UDCA analogue 13 was not more toxic than UDCA. DCA methyl ester 24 was not acting as a prodrug for DCA in this context because it could be recovered unchanged from the medium at 24 h; (v) the 3-deoxy UDCA compound (17) was highly toxic; (vi) apart from the amide 14, the UDCA side chain analogs (18)(19)(20)(21)(22)(23) which are neutral and hydrophobic were not cytotoxic; (vii) oxidation of the steroid secondary alcohols to ketone level was associated with a reduction in cytotoxicity.…”
Section: Effects On Cell Viabilitymentioning
confidence: 99%
“…The similar toxic impact of LCA was observed in C. sinensis newly excysted juveniles [13]. LCA is derived enzymatically from chenodeoxycholic acid and usually present in both human and bovine bile as a minor constituent [21-23]. Thus the worms were unable to tolerate LCA when it was present in higher concentrations (5-10 µM).…”
Section: Discussionmentioning
confidence: 73%