Xenia Effects of Irradiating Corn Pollen in a Nuclear Reactor<sup>1</sup>

Frolik, E. F.; Morris, Rosalind

doi:10.2134/agronj1950.00021962004200060005x

Cited by 3 publications

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“…Generally speaking, in the experiments with X-rays (Stadler and Sprague 1936;Faberge 1957;Nuffer 1957), thermal neutrons (Frolik and Morris 1950) or a-rays (Faberge 1959), the induced mutations occurred more frequently as whole endosperm mutation (7080 0) than as chimeras (2030%). But the UV treatment (Stadler and Sprague 1936;Faberge 1956;Nuffer 1957) showed the reverse relation, namely 7O-.…”

Section: Discussionmentioning

confidence: 99%

DOSE RATE DEPENDENCE OF MUTATION RATES FROM Γ-Irradiated POLLEN GRAINS OF MAIZE

Mabuchi

Matsumura

1964

The Japanese journal of genetics

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Section: Discussionmentioning

confidence: 99%

DOSE RATE DEPENDENCE OF MUTATION RATES FROM Γ-Irradiated POLLEN GRAINS OF MAIZE

Mabuchi

Matsumura

1964

The Japanese journal of genetics

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The Effects of X Rays and Thermal Neutrons on the Development of Trailing Indigo (Indigofera Endecaphylla) Plants

Spencer

Cabanillas

1956

American J of Botany

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DIFFERENT DEVELOPMENT PATTERNS, mostly variations in size, were observed among young plants of trailing indigo (Indigofera endecaphylla Jacq.) arising from seed which had been subjected to various dosages of X rays and thermal neutrons in an attempt to induce beneficial, particularly agronomic, mutations. This study of radiation effects resulted from these observations and the desirability of analyzing the differential growth responses apparently induced by X radiation and thermal neutron flux in dormant seeds of trailing indigo.The existence of a greater quantitative factor, or relative biological efficiency (RBE), for thermal neutrons as compared with X rays is generally recognized. On the basis of equivalent total ionizations, thermal neutrons have about 11 or 12 times the effectivity of X radiation in causing cytogenetic change (Conger and Giles, 1950). In addition to the greater RBE for thermal neutrons as compared with X rays, qualitative differences, different biological effects, have been observed by some workers (Caldecott et al., 1952(Caldecott et al., , 1954 Spencer and Blakeslee, 1%5), and denied by others (Snider, 1948; Frolik and Morris, 1950;Schmidt, 1952). Of primary concern in comparative studies of mutagenic agents is whether or not the biological effects of these agents differ in any fundamental way. The initial purpose of this investigation was to study the nature of the different effects noted in trailing indigo plants arising from seed exposed to X radiation and to thermal neutrons. A second purpose was to find if any apparent difference is related to growth substance activity and/or to protein metabolism.MATERIALS AND METHoDs.-In order to obtain comparative data concerning the responses of trailing indigo plants to dosages of X rays and thermal neutrons, a series of experiments was designed to analyze effects on germination, survival, development, relative growth substance activity, protein metabolism, and pollen production. Seeds of trailing indigo produced at this station were dried to a moisture content of four per cent (-+-0.5 per cent), and used throughout these tests. The self-fertility of this legume assured a high degree of homozygosity (manifest by the uniformity of juvenile and adult characters). Separate lots were treated as follows: 0, and thermal neutrons (total dose) : 0, 7.91 X 10 1 2 , 1.47 X 10 13 , 2.80 X lOIS, 3.43 X 10 13 , 2.11 X 101 3 , 5.04 X 10 1 3 , 6.15 X 10 1 3 , 7.73 X 10 1 3 , 9.84 X 10 1 3 , and 1.30 X 10 Hn/cm. 2 (neutrons per square centimeter). Immediately upon their return, these seeds, together with those retained as additional controls, were scarified with concentrated sulfuric acid for 30 min., washed thoroughly in distilled water, dried, and germinated in Petri dishes or planted in clay pans. Germination percentages were obtained at four days, one week, and two weeks from seeds placed on moist filter paper in Petri dishes and maintained at room temperature (24 0-29 0 C.). Six replicates of 100 seeds to a dish constituted each sample. Data on survival,...

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