Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges

Lei, Ting; Jacob, S.; Ajil-Zaraa, Imen; Dubuisson, Jean‐Bernard; Irion, Olivier; Jaconi, Marisa; Féki, Anis

doi:10.1038/cr.2007.61

Cited by 62 publications

(31 citation statements)

References 64 publications

(88 reference statements)

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“…Undifferentiated cells that retain pluripotency give rise to tumours known as teratomas in vivo (Mitjavila-Garcia et al, 2005). In addition, the difficulties in obtaining HESCs as well as important ethical concerns make the use of HESCs an improbable candidate for TE (Bobbert, 2006;Lei et al, 2007).…”

Section: Human Embryonic Stem Cells As a Source Of Stem Cells For Tementioning

confidence: 99%

Properties of the amniotic membrane for potential use in tissue engineering

Niknejad

Peirovi²,

Jorjani³

et al. 2008

eCM

688

757

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An important component of tissue engineering (TE) is the supporting matrix upon which cells and tissues grow, also known as the scaffold. Scaffolds must easily integrate with host tissue and provide an excellent environment for cell growth and differentiation. Most scaffold materials are naturally derived from mammalian tissues. The amniotic membrane (AM) is considered an important potential source for scaffolding material. The AM represents the innermost layer of the placenta and is composed of a single epithelial layer, a thick basement membrane and an avascular stroma. The special structure and biological viability of the AM allows it to be an ideal candidate for creating scaffolds used in TE. Epithelial cells derived from the AM have the advantages of stem cells, yet are a more suitable source of cells for TE than stem cells. The extracellular matrix components of the basement membrane of the AM create an almost native scaffold for cell seeding in TE. In addition, the AM has other biological properties important for TE, including anti-inflammatory, anti-microbial, anti-fibrosis, anti-scarring, as well as reasonable mechanical property and low immunogenicity. In this review, the various properties of the AM are discussed in light of their potential use for TE.

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Section: Human Embryonic Stem Cells As a Source Of Stem Cells For Tementioning

confidence: 99%

Properties of the amniotic membrane for potential use in tissue engineering

Niknejad

Peirovi²,

Jorjani³

et al. 2008

eCM

688

757

View full text Add to dashboard Cite

show abstract

“…Cells grown in this manner are not ideal for transplantation into humans due to the risk of xenogenic-based rejection by the immune system [18] as well as the potential for cross-species transfer of viruses or pathogens [19]. MEF feeder layers can be replaced with human-derived cell lines to avoid hESC contact with murine cells, but the presence of additional cell lines in hESC culture inevitably contributes to variability and increased cost during the expansion and scale-up of stem cells [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…MEF feeder layers can be replaced with human-derived cell lines to avoid hESC contact with murine cells, but the presence of additional cell lines in hESC culture inevitably contributes to variability and increased cost during the expansion and scale-up of stem cells [19][20][21]. As a result, direct coculture has been replaced by medium conditioning, where hESC are grown on an acellular support matrix in the medium that has been conditioned by a separate, supporting cell line [19,20,22,23].…”

Section: Introductionmentioning

confidence: 99%

Proliferation and Pluripotency of Human Embryonic Stem Cells Maintained on Type I Collagen

Jones

Chu

Pendleton

et al. 2010

Stem Cells and Development

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Human embryonic stem cells (hESC) require a balance of growth factors and signaling molecules to proliferate and retain pluripotency. Conditioned medium (CM) from a human embryonic germ-cell-derived cell culture, SDEC, was observed to support the growth of hESC on type I collagen (COL I) and on Matrigel (MAT) biomatricies. After 1 month, the population doubling of hESC grown in SDEC CM on COL I was equivalent to that of hESC grown in mouse embryonic fibroblast (MEF) CM on MAT. hESC grown in SDEC CM on COL I expressed OCT4, NANOG, SSEA-4, alkaline phosphatase (AP), and TRA-1-60; retained a normal karyotype; and were capable of forming teratomas. DNA microarray analysis was used to compare the transcriptional profiles of SDEC and the less supportive WI38 and Detroit 551 human cell lines. The mRNA level of secreted frizzledrelated protein (sFRP-1), a known antagonist of the WNT=b-catenin signaling pathway, was significantly reduced in SDEC as compared with the other 2 cell lines, whereas the mRNA levels of prostaglandin-endoperoxide synthase 2 (PTGS2 or COX-2) and prostaglandin I 2 synthase (PGIS), two prostaglandin biosynthesis genes, were significantly increased in SDEC. The level of sFRP-1 protein was significantly reduced, and levels of 2 prostaglandins that are downstream products of PTGS2 and PGIS, prostaglandin E 2 and 6-keto-prostaglandin F 1a , were significantly elevated in SDEC CM compared with WI38, Detroit 551, and MEF CM. Further, addition of purified sFRP-1 to SDEC CM reduced the proliferation of hESC grown on COL I as well as MAT in a dosedependent manner.

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“…When transplanted subretinally into a murine model of retinal degenerative disease, these cells are capable of providing partial restoration of retinal function [1,4]. Transplanting human cells exposed to animal-derived products carries an increased risk of infection, disease transmission, immune response, and rejection [16]. For instance, embryonic stem cells cultured on a mouse feeder layer in the presence of bovine serum incorporate the Sialic acid Neu5Gc into their membrane [17].…”

Section: Discussionmentioning

confidence: 99%

Use of a Synthetic Xeno-Free Culture Substrate for Induced Pluripotent Stem Cell Induction and Retinal Differentiation

Tucker

Anfinson

Mullins

et al. 2012

Stem Cells Translational Medicine

103

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The purpose of this study was to determine whether a proprietary xeno-free synthetic culture surface could be used to aid in the production and subsequent retinal-specific differentiation of clinical-grade induced pluripotent stem cells (iPSCs). iPSCs were generated using adult somatic cells via infection with either a single cre-excisable lentiviral vector or four separate nonintegrating Sendai viruses driving expression of the transcription factors OCT4, SOX2, KLF4, and c-MYC. Retinal precursor cells were derived via targeted differentiation of iPSCs with exogenous delivery of dkk-1, noggin, insulin-like growth factor-1, basic fibroblast growth factor, acidic fibroblast growth factor, and DAPT. Phase contrast microscopy, immunocytochemistry, hematoxylin and eosin staining, and reverse transcription-polymerase chain reaction were used to determine reprogramming efficiency, pluripotency, and fate of undifferentiated and differentiated iPSCs. Following viral transduction, cells underwent prototypical morphological changes resulting in the formation of iPSC colonies large enough for manual isolation/passage at 3-4 weeks postinfection. Both normal and disease-specific iPSCs expressed markers of pluripotency and, following transplantation into immune-compromised mice, formed teratomas containing tissue comprising all three germ layers. When subjected to our established retinal differentiation protocol, a significant proportion of the xeno-free substratederived cells expressed retinal cell markers, the number of which did not significantly differ from that derived on traditional extracellular matrix-coated dishes. Synthetic cell culture substrates provide a useful surface for the xeno-free production, culture, and differentiation of adult somatic cell-derived iPSCs. These findings demonstrate the potential utility of these surfaces for the production of clinical-grade retinal neurons for transplantation and induction of retinal regeneration. STEM CELLS TRANSLATIONAL MEDICINE 2013;2:16 -24

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Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges

Cited by 62 publications

References 64 publications

Properties of the amniotic membrane for potential use in tissue engineering

Properties of the amniotic membrane for potential use in tissue engineering

Proliferation and Pluripotency of Human Embryonic Stem Cells Maintained on Type I Collagen

Use of a Synthetic Xeno-Free Culture Substrate for Induced Pluripotent Stem Cell Induction and Retinal Differentiation

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