2023
DOI: 10.1126/sciadv.ade7151
|View full text |Cite
|
Sign up to set email alerts
|

Xeno interactions between MHC-I proteins and molecular chaperones enable ligand exchange on a broad repertoire of HLA allotypes

Abstract: Immunological chaperones tapasin and TAP binding protein, related (TAPBPR) play key roles in antigenic peptide optimization and quality control of nascent class I major histocompatibility complex (MHC-I) molecules. The polymorphic nature of MHC-I proteins leads to a range of allelic dependencies on chaperones for assembly and cell-surface expression, limiting chaperone-mediated peptide exchange to a restricted set of human leukocyte antigen (HLA) allotypes. Here, we demonstrate and characterize xeno interactio… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
26
0

Year Published

2023
2023
2024
2024

Publication Types

Select...
4
1
1

Relationship

3
3

Authors

Journals

citations
Cited by 14 publications
(26 citation statements)
references
References 84 publications
0
26
0
Order By: Relevance
“…We further hypothesized that the interchain disulfide engineering could be applied to different HLA allotypes resulting in a universal, open MHC-I platform for antigen screening experiments. To quantitatively compare peptide exchange rates across different alleles, we then performed a series of FP experiments using optimized placeholder peptides, pHLA concentrations, and the protocol(26), where the binding of high-affinity fluorophore-labeled peptides was monitored through an increase in polarization ( Fig. S6 ).…”
Section: Resultsmentioning
confidence: 99%
See 4 more Smart Citations
“…We further hypothesized that the interchain disulfide engineering could be applied to different HLA allotypes resulting in a universal, open MHC-I platform for antigen screening experiments. To quantitatively compare peptide exchange rates across different alleles, we then performed a series of FP experiments using optimized placeholder peptides, pHLA concentrations, and the protocol(26), where the binding of high-affinity fluorophore-labeled peptides was monitored through an increase in polarization ( Fig. S6 ).…”
Section: Resultsmentioning
confidence: 99%
“…While tapasin has shown preferential binding to HLA-B alleles, TAPBPR preferably interacts with HLA-A alleles but mainly covers the A02 and A24 supertypes (46,63). More recent work has expanded the TAPBPR-mediated peptide exchange on a broad repertoire of allotypes using TAPBPR orthologs and engineered variants (26). However, compared to the open MHC-I platform, the approach requires optimized placeholder peptides and recombinant chaperone proteins to stabilize empty, receptive molecules.…”
Section: Discussionmentioning
confidence: 99%
See 3 more Smart Citations