2017
DOI: 10.1099/jgv.0.000915
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Xenogenic rolling-circle replication of a synthetic beak and feather disease virus genomic clone in 293TT mammalian cells and Nicotiana benthamiana

Abstract: The preparation of infectious beak and feather disease circovirus virions (BFDV) has until now relied on the extraction of virus from whole tissue of deceased or euthanized parrots known to be infected with the virus. Extraction from diseased tissue is necessary, as the virus has yet to be grown in vitro using tissue-cultured cells from any source. While infectious DNA clones have been synthesized for porcine and duck circoviruses, and both replicate in host cells and result in active viral infection in animal… Show more

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Cited by 7 publications
(7 citation statements)
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“…As mentioned previously, while PCV types are culturable, viruses related to BFDV are not-and this significantly hampers vaccine development and study of the viruses' biology. We have recently shown that it is possible to use geminivirus-inspired techniques-making use of partially dimeric genomic clones in E. coli-to show that BFDV genomes can replicate in several animal cell types, and that the same clones expressed via Agrobacterium can be trans-replicated by a co-expressed geminivirus replicon in agroinfiltrated plants (Regnard, de Moor, Hitzeroth, Williamson, & Rybicki, 2017). This represents the first report of a BFDV genome replicating in any cell system, and the first report of any exogenous ssDNA virus genome replicating with the help of a geminivirus replication machinery in a plant.…”
Section: Circovirus Vnps and Pseudovirionsmentioning
confidence: 99%
“…As mentioned previously, while PCV types are culturable, viruses related to BFDV are not-and this significantly hampers vaccine development and study of the viruses' biology. We have recently shown that it is possible to use geminivirus-inspired techniques-making use of partially dimeric genomic clones in E. coli-to show that BFDV genomes can replicate in several animal cell types, and that the same clones expressed via Agrobacterium can be trans-replicated by a co-expressed geminivirus replicon in agroinfiltrated plants (Regnard, de Moor, Hitzeroth, Williamson, & Rybicki, 2017). This represents the first report of a BFDV genome replicating in any cell system, and the first report of any exogenous ssDNA virus genome replicating with the help of a geminivirus replication machinery in a plant.…”
Section: Circovirus Vnps and Pseudovirionsmentioning
confidence: 99%
“…Kessans et al (2016) demonstrated the potential of combining a plant vaccine into a heterologous prime/boost vaccination regimen, in which plant made vaccines, would provide suitable booster vaccines due to the large amount of vaccine material required for repeated booster shots [56]. Others have also demonstrated in animal studies the value that PMP could have for effective vaccine prime/boost strategies [57,58].…”
Section: Conclusion and Perspectivementioning
confidence: 99%
“…Our group has recently published an investigation of the possibility of using circovirus-derived replication control elements to create replicons, or replicating dsDNA plasmid-like molecules, in plants and in mammalian cells [34]. This followed our extensive success with use of a plant ssDNA geminivirus-derived expression vector in plants as an enhanced expression vector [35]: Geminiviruses are very similar to circoviruses in having small circular ssDNA genomes that replicate via a Rep-mediated rolling circle mechanism, and very similar sequences for their non-nucleotide origins of replication (TAATATT/AC vs. TAGTATT/AC).…”
Section: Future Possibilities For Enhanced Dna Vaccine or Expressimentioning
confidence: 99%