Xenon Administration During Early Reperfusion Reduces Infarct Size After Regional Ischemia in the Rabbit Heart In Vivo

Preckel, Benedikt; Müllenheim, Jost; Moloschavij, Andrej; Thämer, V.; Schlack, W.

doi:10.1097/00000539-200012000-00003

Cited by 104 publications

(63 citation statements)

References 23 publications

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“…In two additional groups, we investigated whether we could induce cardioprotection in ZO rats by helium postconditioning. In these groups, ZL and ZO rats (each n ϭ 8) received 70% helium/30% oxygen for 15 min at the onset of reperfusion (Preckel et al, 2000).…”

Section: Experimental Protocolmentioning

confidence: 99%

Helium-Induced Early Preconditioning and Postconditioning Are Abolished in Obese Zucker Rats in Vivo

Huhn¹,

Heinen²,

Weber³

et al. 2009

J Pharmacol Exp Ther

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Preconditioning is abolished in the prediabetic Zucker obese rat. It has been shown that prevention of mitochondrial permeability transition pore (mPTP) opening is involved in preconditioning by the noble gas helium. Here, we investigated: 1) whether helium induces pre-and postconditioning in Zucker rats and 2) whether possible regulators of the mPTP [i.e., mitochondrial respiration or the extracellular signal-regulated kinase (Erk) 1/2, Akt/glycogen synthase kinase (GSK)-3␤ signaling pathway] are influenced. Anesthetized Zucker lean (ZL) and Zucker obese (ZO) rats were randomized to seven groups. Control animals were not treated (ZL-/ZO-Con). Preconditioning groups (ZL-/ZO-He-PC) inhaled 70% helium for 3 ϫ 5 or 6 ϫ 5 min, and postconditioning groups (ZL-/ZO-He-PostC) inhaled 70% helium for 15 min at the onset of reperfusion. Animals underwent 25 min of ischemia and 120 min of reperfusion. In additional experiments, hearts were excised after the third helium exposure for analysis of mitochondrial respiration and for Western blot analysis of Erk1/2, Akt, and GSK-3␤ phosphorylation. Helium reduced infarct size from 52 Ϯ 3% (mean Ϯ S.E.) to 32 Ϯ 2% and 37 Ϯ 2% in ZL rats (ZL-HE-PC, ZL-He-PostC), respectively, but not in ZO rats [ZO-He-PC, 56 Ϯ 3%; ZO-He-PC (6ϫ), 57 Ϯ 4%; and ZO-He-PostC, 51 Ϯ 3% versus ZO-Con, 54 Ϯ 3%]. Mitochondrial respiration analysis showed that helium causes mild uncoupling in ZL rats (2.27 Ϯ 0.03 versus 2.51 Ϯ 0.03) but not in ZO rats (2.52 Ϯ 0.04 versus 2.52 Ϯ 0.03). Helium had no effect on Erk1/2 and Akt phosphorylation. GSK-3␤ phosphorylation during ischemia was reduced after helium application in ZL but not in ZO rats. Helium-induced preconditioning is abolished in obese Zucker rats in vivo, probably caused by a diminished effect of helium on mitochondrial respiration.

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Section: Experimental Protocolmentioning

confidence: 99%

Helium-Induced Early Preconditioning and Postconditioning Are Abolished in Obese Zucker Rats in Vivo

Huhn¹,

Heinen²,

Weber³

et al. 2009

J Pharmacol Exp Ther

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“…32 The inhalational anesthetic xenon has also been shown to reduce infarct size in rabbits. 33 Due to the varying effects of different anesthetics on the cardiovascular system, we opted to compare isoflurane and propofol general anesthesia during an acute myocardial infarction directly, rather than creating a true control group. In doing so we found only short-term cardiovascular differences between the two groups.…”

Section: Infarct Ratiomentioning

confidence: 99%

Similar long-term cardiovascular effects of propofol or isoflurane anesthesia during ischemia/ reperfusion in dogs

Thompson

Wisenberg

Sykes

et al. 2002

Can J Anesth/J Can Anesth

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P Pu ur rp po os se e: : To compare the long-term functional and metabolic effects of propofol or isoflurane general anesthesia in a canine model of ischemia/reperfusion. M Me et th ho od ds s: :Using magnetic resonance (MR) techniques, we monitored both regional metabolism ( 31 P MR spectroscopy) and systolic function of the heart ( 1 H MR imaging) throughout a two-hour occlusion of the left anterior descending coronary artery and ten days of reperfusion. Twenty-two beagles were randomized into isoflurane and propofol general anesthesia groups (n = 10, n = 12 respectively). Contrast-enhanced MR imaging was used to measure infarct size (% of left ventricle that was necrotic) and coronary blood flow was determined using radioactively labelled microspheres.R Re es su ul lt ts s: : Cardiac metabolism, as monitored by intracellular pH and high-energy phosphate ratios, was not significantly different between the two groups throughout the protocol. Relative to propofol, isoflurane reduced the depression of left ventricular ejection fraction (EF) during the ischemic period [isoflurane 68.5% ± 4.2%, propofol 58.3% ± 2.0% of baseline (B); P = 0.04], propofol increased the recovery of EF at day three (isoflurane 63.9% ± 4.3%, propofol 74.0% ± 2.5% of B; P = 0.05). By day ten, EF in both groups was similar. Infarct sizes were also similar at day ten (isoflurane 15.7% ± 3.0%, propofol 13.2% ± 2.2%). Normalizing these by the region at risk (volume of tissue with low blood flow during the occlusion) to assess infarct ratios was also not significant (isoflurane 0.58% ± 0.08%, propofol 0.54% ± 0.07%).C Co on nc cl lu us si io on ns s: : There were no significant differences between the two anesthetic groups at day ten, suggesting that any apparent dissimilarities in early cardiovascular effects were short-term only. These results indicate that isoflurane and propofol produce equivalent long-term cardiovascular effects following ischemia/reperfusion. [isoflurane : 68,5 %± 4,2 %, propofol : 58,3 % ± 2,0 % des données de base (B) ; P = 0,04], le propofol a accéléré la récupération de la FE au jour trois (isoflurane : 63,9 % ± 4,3 %, propofol : 74,0 % ± 2,5 % de B ; P = 0,05). Au dixième jour, la FE était similaire dans les deux groupes, de même la taille de l'infarctus (isoflurane : 15,7 % ± 3,0 %, propofol : 13,2 % ± 2,2 %). La normalisation de ces données, selon la région à risque (volume de tissu soumis à un faible débit sanguin pendant l'occlusion), réalisée pour évaluer les ratios d'infarctus n'a pas été significative non plus (isoflurane : 0,58 % ± 0,08 %, propofol : 0,54 % ± 0,07 %). Objectif : Comparer les effets fonctionnels et métaboliques prolongés de l'anesthésie générale avec du propofol ou de l'isoflurane chez un modèle canin d'ischémie/reperfusion. Méthode : Nous avons mesuré, grâce aux techniques d'imagerie par résonance magnétique (IRM), le métabolisme régional (spectroscopie IRM 31P) et la fonction systolique cardiaque (IRM 1H) tout au long d'une occlusion de deux heures de l'artère descendante antérieure et...

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“…Preckel et al observaram, em modelos animais de enfarte agudo do miocárdio in vivo, uma redução da área de enfarte com a administração de xénon, quer antes do evento isquémico, 29 quer durante o período de reperfusão. 30 Estas características favorecem a aplicação em doentes e situações com compromisso cardiovascular, como na cirurgia cardíaca, nas cirurgias emergentes e no choque. Todavia, a ausência de estudos de larga escala em doentes ASA III e IV impossibilita a sua aplicação neste contexto.…”

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Como Funciona o Xénon: Mecanismos de Neuro e Cardioprotecção

et al. 2014

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Xenon Administration During Early Reperfusion Reduces Infarct Size After Regional Ischemia in the Rabbit Heart In Vivo

Cited by 104 publications

References 23 publications

Helium-Induced Early Preconditioning and Postconditioning Are Abolished in Obese Zucker Rats in Vivo

Helium-Induced Early Preconditioning and Postconditioning Are Abolished in Obese Zucker Rats in Vivo

Similar long-term cardiovascular effects of propofol or isoflurane anesthesia during ischemia/ reperfusion in dogs

Como Funciona o Xénon: Mecanismos de Neuro e Cardioprotecção

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