Xenopus as a model system for studying pancreatic development and diabetes

Kofent, Julia; Spagnoli, Francesca M.

doi:10.1016/j.semcdb.2016.01.005

Cited by 10 publications

(5 citation statements)

References 132 publications

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“…The use of Xenopus eggs and embryos for in vivo analyses of disease gene expression and function has exploded in recent years. This model has supported important advances in our understanding of: neurological disorders including autism, Alzheimer's disease, and depression (James et al, ; Park et al, ; Ramirez‐Vizcarrando, Hasan, Gu, Khakhalin, & Aizenman, ; Ullah, Demuro, Parker, & Pearson, ); cancers (Green, Kwon, & Christian, ; Haynes‐Gilmore et al, ; Van Nieuwenhuysen et al, ; Wei et al, ); congenital heart defects (Endicott, Basu, Khokha, & Brueckner, ; Silva et al, ; Torres‐Prioris, Smith, Mohun, Fernández, & Durán, ); craniofacial and auditory malformations (Dickinson, ; Griffin, Sondalle, Del Viso, Baserga, & Khokha, ; Moody, Neilson, Kenyon, Alfandari, & Pignoni, ; Ramírez‐Gordillo et al, ); diabetes (Kofent & Spagnoli, ; Pearl, Jarikji, & Horb, ; Salanga & Horb, ); kidney disease (Desgrange & Cereghini, ; Lienkamp, ; Stiburkova, Stekrova, Nakamura, & Ichida, ); Lennox–Gastaut syndrome (Hammer, Ebert, Jensen, & Jensen, ); and Zimmermann–Laband syndrome (Kortüm et al, ).…”

Section: Xenopus Is a Powerful System For Deciphering The Function Ofmentioning

confidence: 83%

Using Xenopus to understand human disease and developmental disorders

Sater

Moody

2017

Genesis

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Model animals are crucial to biomedical research. Among the commonly used model animals, the amphibian, Xenopus, has had tremendous impact because of its unique experimental advantages, cost effectiveness, and close evolutionary relationship with mammals as a tetrapod. Over the past 50 years, the use of Xenopus has made possible many fundamental contributions to biomedicine, and it is a cornerstone of research in cell biology, developmental biology, evolutionary biology, immunology, molecular biology, neurobiology, and physiology. The prospects for Xenopus as an experimental system are excellent: Xenopus is uniquely well-suited for many contemporary approaches used to study fundamental biological and disease mechanisms. Moreover, recent advances in high throughput DNA sequencing, genome editing, proteomics, and pharmacological screening are easily applicable in Xenopus, enabling rapid functional genomics and human disease modeling at a systems level.

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Section: Xenopus Is a Powerful System For Deciphering The Function Ofmentioning

confidence: 83%

Using Xenopus to understand human disease and developmental disorders

Sater

Moody

2017

Genesis

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“…Another widely used laboratory model is the clawed frog (Xenopus laevis). Primarily used for developmental cardiac questions, this aquatic inhabitant is also used, among other things, for the study of genetic diabetes with attention to pancreatic development (66,67). Research in Xenopus is focused on congenital heart disease, long QT syndrome, hypertension and atrial fibrillation, whereby genetic modifications of cardiac ion channels can be studied in particular in oocytes.…”

Section: Non-mammalian Modelsmentioning

confidence: 99%

Research('s) Sweet Hearts: Experimental Biomedical Models of Diabetic Cardiomyopathy

Richter

Hinkel

2021

Front. Cardiovasc. Med.

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Diabetes and the often accompanying cardiovascular diseases including cardiomyopathy represent a complex disease, that is reluctant to reveal the molecular mechanisms and underlying cellular responses. Current research projects on diabetic cardiomyopathy are predominantly based on animal models, in which there are not only obvious advantages, such as genetics that can be traced over generations and the directly measurable influence of dietary types, but also not despisable disadvantages. Thus, many studies are built up on transgenic rodent models, which are partly comparable to symptoms in humans due to their genetic alterations, but on the other hand are also under discussion regarding their clinical relevance in the translation of biomedical therapeutic approaches. Furthermore, a focus on transgenic rodent models ignores spontaneously occurring diabetes in larger mammals (such as dogs or pigs), which represent with their anatomical similarity to humans regarding their cardiovascular situation appealing models for testing translational approaches. With this in mind, we aim to shed light on the currently most popular animal models for diabetic cardiomyopathy and, by weighing the advantages and disadvantages, provide decision support for future animal experimental work in the field, hence advancing the biomedical translation of promising approaches into clinical application.

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“…Diabetes mellitus was the predominant condition annotated for diseases of metabolism ( n = 44) with experiments utilizing either the embryos or oocytes. Pancreas development is highly conserved between Xenopus and mammals, making it an ideal model to study and screen genetic candidates involved in congenital pancreas defects (Kofent and Spagnoli, 2016) such as those occurring in type 1 diabetes mellitus. Simaite et al (2014) took advantage of this high conservation with a combination of whole-genome sequencing with linkage analysis in a consanguineous family with early onset antibody-negative diabetes and morpholino knockdown of candidate orthologous genes in Xenopus to identify a variant in the patient gene PCBD1 as the likely cause of pancreatic insufficiency and type 1 diabetes in these families.…”

Section: The Landscape Of Human Disease Research In Xenopusmentioning

confidence: 99%

Xenbase: Facilitating the Use of Xenopus to Model Human Disease

Fisher

James-Zorn

et al. 2019

Front. Physiol.

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At a fundamental level most genes, signaling pathways, biological functions and organ systems are highly conserved between man and all vertebrate species. Leveraging this conservation, researchers are increasingly using the experimental advantages of the amphibian Xenopus to model human disease. The online Xenopus resource, Xenbase, enables human disease modeling by curating the Xenopus literature published in PubMed and integrating these Xenopus data with orthologous human genes, anatomy, and more recently with links to the Online Mendelian Inheritance in Man resource (OMIM) and the Human Disease Ontology (DO). Here we review how Xenbase supports disease modeling and report on a meta-analysis of the published Xenopus research providing an overview of the different types of diseases being modeled in Xenopus and the variety of experimental approaches being used. Text mining of over 50,000 Xenopus research articles imported into Xenbase from PubMed identified approximately 1,000 putative disease- modeling articles. These articles were manually assessed and annotated with disease ontologies, which were then used to classify papers based on disease type. We found that Xenopus is being used to study a diverse array of disease with three main experimental approaches: cell-free egg extracts to study fundamental aspects of cellular and molecular biology, oocytes to study ion transport and channel physiology and embryo experiments focused on congenital diseases. We integrated these data into Xenbase Disease Pages to allow easy navigation to disease information on external databases. Results of this analysis will equip Xenopus researchers with a suite of experimental approaches available to model or dissect a pathological process. Ideally clinicians and basic researchers will use this information to foster collaborations necessary to interrogate the development and treatment of human diseases.

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Xenopus as a model system for studying pancreatic development and diabetes

Cited by 10 publications

References 132 publications

Using Xenopus to understand human disease and developmental disorders

Using Xenopus to understand human disease and developmental disorders

Research('s) Sweet Hearts: Experimental Biomedical Models of Diabetic Cardiomyopathy

Xenbase: Facilitating the Use of Xenopus to Model Human Disease

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