Xenopus Germes encodes a novel germ plasm-associated transcript

Berekelya, Lyubov A.; Ponomarev, M. B.; Luchinskaya, N. N.; Belyavsky, A. V.

doi:10.1016/s1567-133x(03)00055-3

Cited by 32 publications

(21 citation statements)

References 9 publications

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“…This unusual germ plasm morphology in GermesΔLZs‐expressing embryos was also clear in the light microscopy of Xpat RNA in situ hybridizations. Having moved from subcortical localization to a perinuclear position of PGC at the onset of gastrulation, germ plasm is located in the yolk‐free cytoplasm around its nucleus (Blackler, 1958; Houston et al, 1998b; Berekelya et al, 2003). At later stages (31–33), Xpat RNA‐containing germ plasm seems to disperse between yolk granules around the nucleus and the yolk‐free cytoplasm is usually absent.…”

Section: Discussionmentioning

confidence: 99%

The protein encoded by the germ plasm RNA Germes associates with dynein light chains and functions in Xenopus germline development

et al. 2007

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Section: Discussionmentioning

confidence: 99%

The protein encoded by the germ plasm RNA Germes associates with dynein light chains and functions in Xenopus germline development

et al. 2007

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“…Other known vegetal cortex RNAs, xlsirt 13.2 (Kloc et al, 1993) and xvelo1 (Claussen and Pieler, 2004) do not have antisense targets on the array. Probe ID is the Affymetrix probe set number. References: [1] Betley et al (2002); [2] Mosquera et al (1993); [3] Berekelya et al (2003); [4] Kaneshiro et al (2007); [5] Tarbashevich et al (2007); [6] MacArthur et al (2000); [7] King et al (2005); [8] Wessely and De Robertis (2000); [9] Ku and Melton (1993); [10] Kloc et al (1998); [11] Zhou et al (2004); [12] Houston et al (1998); [13] Pannese et al (2000); [14] Hudson and Woodland (1998); [15] Claussen et al (2004); [16] Weidinger et al (2003); [17] Hudson et al (1996); [18] Chan et al (2001); [19] Rebagliati et al (1985); [20] Weeks and Melton (1987); [21] Kloc and Chan (2007); [22] Zhang and King (1996); [23] Zearfoss et al (2004). Germ plasm-localized transcripts are shaded. …”

Section: Figures and Tablementioning

confidence: 99%

Identification of germ plasm‐associated transcripts by microarray analysis of Xenopus vegetal cortex RNA

Cuykendall

Houston

2010

Developmental Dynamics

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RNA localization is a common mechanism for regulating cell structure and function. Localized RNAs in Xenopus oocytes are critical for early development, including germline specification by the germ plasm. Despite the importance of these localized RNAs, only approximately 25 have been identified and fewer are functionally characterized. Using microarrays, we identified a large set of localized RNAs from the vegetal cortex. Overall, our results indicate a minimum of 275 localized RNAs in oocytes, or 2-3% of maternal transcripts, which are in general agreement with previous findings. We further validated vegetal localization for 24 candidates and further characterized three genes expressed in the germ plasm. We identified novel germ plasm expression for reticulon 3.1, exd2 (a novel exonuclease-domain encoding gene), and a putative noncoding RNA. Further analysis of these and other localized RNAs will likely identify new functions of germ plasm and facilitate the identification of cis-acting RNA localization elements. Developmental Dynamics 239:1838-1848,

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“…Some of these have now been confirmed by in situ hybridization as being specifically localized in the MC and later in oocyte germ plasm islands or embryonic germ plasm. These include DeadSouth, Xdazl, Hermes, Germes, Dead-end, and Xpat (Berekelya et al 2003;Houston and King 2000a;MacArthur et al 2000;Weidinger et al 2003;Zearfoss et al 2004) and are listed in Table 5.1. mRNAs encoding RNA-binding proteins include the zinc finger protein Nanos; RRM containing RNAs Xdazl, dead-end, and hermes; and helicases DeadSouth and Centroid.…”

Section: Molecular Components Of Germ Plasmmentioning

confidence: 99%

Germ‐Cell Specification inXenopus

King

2014

Xenopus Development

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Primordial germ cells (PGCs) represent the only precursor stem cell population that normally gives rise to the gametes. For this reason, PGCs have been called the "stem cells of the species" and are prime examples of a normal totipotent state (Wylie, 1999. Cell 96 (2): 165-174). PGCs represent a remarkable lineage that will differentiate into highly specialized cells, either oocytes or sperm, yet also retain the ability to generate a complete new individual. Thus, a fundamental question in developmental and stem cell biology is how PGCs differentiate while retaining the potential for totipotency in contrast to somatic cells that become fate-restricted. In this chapter, we will review what is known about how Xenopus sets aside its germ-cell lineage.

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Xenopus Germes encodes a novel germ plasm-associated transcript

Cited by 32 publications

References 9 publications

The protein encoded by the germ plasm RNA Germes associates with dynein light chains and functions in Xenopus germline development

The protein encoded by the germ plasm RNA Germes associates with dynein light chains and functions in Xenopus germline development

Identification of germ plasm‐associated transcripts by microarray analysis of Xenopus vegetal cortex RNA

Germ‐Cell Specification inXenopus

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