Xenoreactive CD4<sup>+</sup> memory T cells resist inhibition by anti‐CD44 mAb and reject islet grafts via a Th2‐dependent pathway

Peng, Yuanzheng; Chen, Jibing; Shao, Wei; Wang, Feiyu; Dai, Helong; Cheng, Panpan; Xia, Junjie; Wang, Feng; Huang, Ruxin; Zhu, Qi; Qi, Zhongquan

doi:10.1111/j.1399-3089.2011.00646.x

Cited by 9 publications

(6 citation statements)

References 40 publications

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“…Recipient mice were treated with 180 mg/kg streptozotocin (STZ) intra-peritoneally (IP) to induce the diabetes model 18 . After three days, mice with a blood glucose level higher than 16.7 mM in two consecutive days were used for transplantation.…”

Section: Methodsmentioning

confidence: 99%

Immunosuppressive effect of arsenic trioxide on islet xenotransplantation prolongs xenograft survival in mice

et al. 2018

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The role of arsenic trioxide (As2O3) in inhibiting immune rejection and prolonging islet allograft survival has been identified in islet allotransplantation. This study aims to explore the role of As2O3 in islet xenotransplantation and the action mechanism. The streptozotocin (STZ) was used in C57BL/6 mice to induce the type 1 diabetes mellitus (T1DM) for xenotransplantation models establishment. Donor islets were isolated by digesting. The flow cytometry (FCM) was used to analyze lymphocyte types. The blood sugar level was detected by using intraperitoneal glucose tolerance test (IPGTT). The serum level of cytokines was determined by the enzyme-linked immunosorbent assay (ELIZA). The cell proliferation was measured by MTT assay. The mRNA levels were quantified with qRT-PCR. As2O3 prolonged the survival of the recipient mice but had no influence on body weight. As2O3 protected the function of xenograft in insulin secretion and suppressed immune rejection of recipient. As2O3 inhibited proliferation of T lymphocyte and increased the proportion of Foxp3+ regulatory T cells in recipient mice. As2O3 inhibited activation and promoted clonal anergy of T lymphocyte. As2O3 decreased total number of B cells and reduced partial antibody levels in recipient mice. As2O3 and leflunomide showed a synergistic effect in suppressing islet xenotransplant rejection. As2O3 prolongs islet xenograft survival by inhibiting cellular immune response, and increasing Foxp3+ regulatory T cells, while decreasing partial antibody levels in serum.

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Section: Methodsmentioning

confidence: 99%

Immunosuppressive effect of arsenic trioxide on islet xenotransplantation prolongs xenograft survival in mice

et al. 2018

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“…injection of 180 mg/kg streptozotocin (Sigma-Aldrich). 19 Three days later, mice with blood glucose levels greater than 16.7 mM on 2 consecutive days were used for transplantation. Four hundred islets were transplanted under the kidney capsule of the recipient mice.…”

Section: Induction Of Diabetes and Islet Transplantationmentioning

confidence: 99%

Arsenic Trioxide Induces T Cell Apoptosis and Prolongs Islet Allograft Survival in Mice

Gao

Jiang

et al. 2015

Transplantation

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“…A recent study in a non-autoimmune model demonstrated that administration of anti-CD44 prolonged survival of allogeneic islets after transplantation (Peng et al, 2011). This improvement was associated with greatly reduced accumulation of CD4+ T cells within the grafts and diminished levels of IFN-γ in the sera of treated mice.…”

Section: Targeting Cd44 On T Cells To Ameliorate Disease: the Examplementioning

confidence: 99%

Regulation of Antigen-Experienced T Cells: Lessons from the Quintessential Memory Marker CD44

Baaten¹,

Tinoco²,

Chen³

et al. 2012

Front. Immun.

117

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Despite the widespread use of the cell-surface receptor CD44 as a marker for antigen (Ag)-experienced, effector and memory T cells, surprisingly little is known regarding its function on these cells. The best-established function of CD44 is the regulation of cell adhesion and migration. As such, the interactions of CD44, primarily with its major ligand, the extracellular matrix (ECM) component hyaluronic acid (HA), can be crucial for the recruitment and function of effector and memory T cells into/within inflamed tissues. However, little is known about the signaling events following engagement of CD44 on T cells and how cooperative interactions of CD44 with other surface receptors affect T cell responses. Recent evidence suggests that the CD44 signaling pathway(s) may be shared with those of other adhesion receptors, and that these provide contextual signals at different anatomical sites to ensure the correct T cell effector responses. Furthermore, CD44 ligation may augment T cell activation after Ag encounter and promote T cell survival, as well as contribute to regulation of the contraction phase of an immune response and the maintenance of tolerance. Once the memory phase is established, CD44 may have a role in ensuring the functional fitness of memory T cells. Thus, the summation of potential signals after CD44 ligation on T cells highlights that migration and adhesion to the ECM can critically impact the development and homeostasis of memory T cells, and may differentially affect subsets of T cells. These aspects of CD44 biology on T cells and how they might be modulated for translational purposes are discussed.

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Xenoreactive CD4⁺ memory T cells resist inhibition by anti‐CD44 mAb and reject islet grafts via a Th2‐dependent pathway

Cited by 9 publications

References 40 publications

Immunosuppressive effect of arsenic trioxide on islet xenotransplantation prolongs xenograft survival in mice

Immunosuppressive effect of arsenic trioxide on islet xenotransplantation prolongs xenograft survival in mice

Arsenic Trioxide Induces T Cell Apoptosis and Prolongs Islet Allograft Survival in Mice

Regulation of Antigen-Experienced T Cells: Lessons from the Quintessential Memory Marker CD44

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Xenoreactive CD4+ memory T cells resist inhibition by anti‐CD44 mAb and reject islet grafts via a Th2‐dependent pathway

Cited by 9 publications

References 40 publications

Immunosuppressive effect of arsenic trioxide on islet xenotransplantation prolongs xenograft survival in mice

Immunosuppressive effect of arsenic trioxide on islet xenotransplantation prolongs xenograft survival in mice

Arsenic Trioxide Induces T Cell Apoptosis and Prolongs Islet Allograft Survival in Mice

Regulation of Antigen-Experienced T Cells: Lessons from the Quintessential Memory Marker CD44

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Xenoreactive CD4⁺ memory T cells resist inhibition by anti‐CD44 mAb and reject islet grafts via a Th2‐dependent pathway