Xenotransplantation of Hepatocytes in Rats with Acute Liver Failure Using Sirolimus for Immunosuppression

Lytras, Dimitrios; Papalois, Αpostolos; Tsaroucha, AK; Papagoras, Dimitrios; Kyriazanos, John; Lambropoulou, María; Giannakou, Niki; Galanos, Antonis; Ce, Simopoulos

doi:10.1177/147323001003800217

Cited by 2 publications

(2 citation statements)

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“…The use of more modern immunosuppressive drugs, was a question with significant experimental value [25]. Lytras et al evaluated the effect of sirolimus alone or in combination with cyclosporin, as an immunosuppressant during xenotransplantation of rabbit hepatocytes into Wistar male rats with acute liver failure [26]. Ginesta et al evaluated the ability of thePAF antagonist UR-12670 to improve graft function in late xenograft rejection (LXR) in anorthotopic liver xenotransplantation model, and the involvement of PAF (platelet activating factor) in this type of rejection [27].…”

Section: Discussionmentioning

confidence: 99%

Mycophenolate Mofetil and Sirolimus in Hepatocyte Transplantation in an Experimental Model of Toxic Acute Liver Failure

Loukopoulos

Sfiniadakis

Pillai³

et al. 2014

Journal of Investigative Surgery

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Section: Discussionmentioning

confidence: 99%

Mycophenolate Mofetil and Sirolimus in Hepatocyte Transplantation in an Experimental Model of Toxic Acute Liver Failure

Loukopoulos

Sfiniadakis

Pillai³

et al. 2014

Journal of Investigative Surgery

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“…Makowka [117] Rat (ALF) Intraperitoneal 4 9 10 7 Presensitization did not affect survival Makowka [118] Rat (ALF) Spleen 1 9 10 8 Hepatocyte graft survival was improved by daclizumab Papagoras [132] Rat (ALF) Spleen 1 9 10 8 Rapamycin offered no survival advantage Lytras [133] ALF, acute liver failure. Mouse (uPA/SCID) Spleen 5.0-7.5 9 10 6 Hepatocytes maintained their viability Hata [137] Alb-uPA mice: albumin-urokinase (Alb-uPA) transgenic mice; DTH: the delayed-type hypersensitivity; Jo2: specific anti-mouse Fas monoclonal antibody; ALF, acute liver failure; SCID, severe combined immunodeficieny disease.…”

Section: Resultsmentioning

confidence: 99%

Experimental hepatocyte xenotransplantation—a comprehensive review of the literature

Zhou

Liu

Ezzelarab

et al. 2015

Xenotransplantation

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Background Hepatocyte transplantation is a potential therapy for certain diseases of the liver, including hepatic failure. However, there is a limited supply of human livers as a source of cells and, after isolation, human hepatocytes can be difficult to expand in culture, limiting the number available for transplantation. Hepatocytes from other species, e.g., the pig, have therefore emerged as a potential alternative source. We searched the literature through the end of 2014 to assess the current status of experimental research into hepatocyte xenotransplantation. Literature search and results The literature search identified 51 reports of in vivo cross-species transplantation of hepatocytes in a variety of experimental models. Most studies investigated the transplantation of human (n=23) or pig (n=19) hepatocytes. No studies explored hepatocytes from genetically-engineered pigs. The spleen was the most common site of transplantation (n=23), followed by the liver (through the portal vein [n=6]) and peritoneal cavity (n=19). In 47 studies (92%), there was evidence of hepatocyte engraftment and function across a species barrier. Conclusions The data provided by this literature search strengthen the hypothesis that xenotransplantation of hepatocytes is feasible and potentially successful as a clinical therapy for certain liver diseases, including hepatic failure. By excluding vascular structures, hepatocytes isolated from genetically-engineered pig livers may address some of the immunological problems of xenotransplantation.

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Xenotransplantation of Hepatocytes in Rats with Acute Liver Failure Using Sirolimus for Immunosuppression

Cited by 2 publications

References 31 publications

Mycophenolate Mofetil and Sirolimus in Hepatocyte Transplantation in an Experimental Model of Toxic Acute Liver Failure

Mycophenolate Mofetil and Sirolimus in Hepatocyte Transplantation in an Experimental Model of Toxic Acute Liver Failure

Experimental hepatocyte xenotransplantation—a comprehensive review of the literature

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