Xerovac: an ultra rapid method for the dehydration and preservation of live attenuated Rinderpest and Peste des Petits ruminants vaccines

Worrall, E.E.; Litamoi, J. K.; Seck, B.M; Ayelet, Gelagay

doi:10.1016/s0264-410x(00)00229-2

Cited by 76 publications

(47 citation statements)

References 8 publications

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“…However at 37°C, orf vaccine stabilized with LHT stabilizer showed better stability than that of LS and TAA. Earlier studies have indicated that the increased concentration of trehalose dihydrate prolonged the stability of TCRP and PPR vaccines (Worrall et al, 2000). It is known that the trehalose protects cell membrane, protein and other molecules from drying and also preserve the natural biological activity of the molecules.…”

Section: Discussionmentioning

confidence: 99%

“…It forms protective layer around the protein and other biomolecules protecting biological/s under adverse conditions like extreme temperatures, hypertonia and dehydration. Trehalose has become nature's dessiprotectant (Worrall et al, 2000). In a desiccating environment, trehalose dries as a transparent glass and results in vitrification.…”

Section: Discussionmentioning

confidence: 99%

“…Estimation of residual moisture: Thermo-gravimetric method was followed for estimation of Residual Moisture (RM) of the lyophilized vaccines (Worrall et al, 2000). Accordingly, the mean weight of 10 vials from each vaccine batch was taken and then dried at 80°C for 20 h and the weight of water loss from the dried vaccine was expressed as percentage.…”

Section: Virus Titrationmentioning

confidence: 99%

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Effect of Stabilization and Reconstitution on the Stability of a Novel Strain of Live Attenuated Orf Vaccine (ORFV MUK59/05)

Bora¹,

Bhanuprakash²,

Venkatesan³

et al. 2015

Asian J. of Animal and Veterinary Advances

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The study describes the effect of stabilization on the stability of an indigenous live attenuated orf vaccine with stabilizers like LS, LHT and TAA after lyophilization and also reconstitution with NSS, PBS, distilled water and 1 M MgSO 4 exposed at different temperatures under established lyophilization conditions. The stability of the vaccine was assessed both in freeze-dried and reconstituted forms at different temperatures. The results indicated that the orf vaccine lyophilized with LS stabilizer at 25 and 45°C and LHT at 37°C found superior over others in stabilizing the keeping quality of the vaccine. Intrinsic thermo-stability studies revealed the rapid deterioration of the vaccine, when compared to the vaccine stabilized with the either of the stabilizers. Among the diluents used for reconstitution of the vaccine, quality in terms of infectivity titers of the virus was well preserved in vaccine diluted with 0.85% NSS compared to other diluents like PBS, DW and 1 M MgSO 4 . The 1 M MgSO 4 found unsuitable for diluting the orf vaccine. The study suggests that the LS at 25 and 45°C and LHT at 37°C are the choice of stabilizers and 0.85% NSS is the choice of diluent for orf vaccine at all temperatures contemplated.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Virus Titrationmentioning

confidence: 99%

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Effect of Stabilization and Reconstitution on the Stability of a Novel Strain of Live Attenuated Orf Vaccine (ORFV MUK59/05)

Bora¹,

Bhanuprakash²,

Venkatesan³

et al. 2015

Asian J. of Animal and Veterinary Advances

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“…This assay detects PPRV antibodies in serum samples by quantifying the amount of MAb after 30 min of incubation of a serum-MAb conjugate mixture on plates coated with a PPRV recombinant N protein (rPPRV-N) expressed in baculovirus system. The expressed PPRV F protein in bacterial [51] and eukaryotic [80] system showed antigenicity and immunogenicity [159]. Further, the expressed truncated and full-length N protein of PPRV in E. coli showed reactivity in s-ELISA and tested as a coating antigen in c-ELISA for serological diagnosis of PPR infection [161].…”

Section: Recombinant Antigen Based Assaysmentioning

confidence: 99%

“…In a cross protection study, PPR vaccine was found to protect cattle effectively against RP [34]. It has been demonstrated that freeze-drying of this vaccine in an excipient containing trehalose makes it very thermostable and it resists temperature up to 45°C for a period of 14 days with minimal loss of potency [159]. The genetic diversity among the PPR viruses occurring in different geographic locations has already been established [39,128].…”

Section: Prevention and Controlmentioning

confidence: 99%

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

135

118

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Peste des petits ruminants (PPR) is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality and caused by PPR virus. PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and particularly severely affects poor farmer's economy. The disease is clinically manifested by pyrexia, oculo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhoea and bronchopneumonia. The disease can be diagnosed from its clinical signs, pathological lesions, and specific detection of virus antigen/antibodies/genome in the clinical samples by various serological tests and molecular assays. PPR is the one of the priority animal diseases whose control is considered important for poverty alleviation in enzootic countries. Availability of effective and safe live attenuated cell culture PPR vaccines and diagnostics have boosted the recently launched centrally sponsored control programme in India and also in other countries. This review article primarily focus on the current scenario of PPR diagnosis and its control programme with advancement of research areas that have taken place in the recent years with future perspectives.

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Drying Process Methods for Biopharmaceutical Products: An Overview

Abdul‐Fattah

Truong

2010

Formulation and Process Development Strategies for Manufacturing Biopharmaceuticals

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Xerovac: an ultra rapid method for the dehydration and preservation of live attenuated Rinderpest and Peste des Petits ruminants vaccines

Cited by 76 publications

References 8 publications

Effect of Stabilization and Reconstitution on the Stability of a Novel Strain of Live Attenuated Orf Vaccine (ORFV MUK59/05)

Effect of Stabilization and Reconstitution on the Stability of a Novel Strain of Live Attenuated Orf Vaccine (ORFV MUK59/05)

Diagnosis and control of peste des petits ruminants: a comprehensive review

Drying Process Methods for Biopharmaceutical Products: An Overview

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