xgrid3d: A free software tool for quantitative image analysis and internal dosimetry

Bolaños-Puchet, Sirio

doi:10.1063/1.5095898

Cited by 1 publication

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“…The workflow for postprocessing of reconstructed images employed the in‐house software xgrid3d 13 and consisted of the following steps: region of interest (ROI) selection (the subarachnoid space at the vertebral segment of the spinal level where the contrast agent concentrated), image registration (using, as reference, the image obtained after contrast injection to reduce information loss), image weighted subtraction, and alignment. Additionally, 3‐dimensional contour renderings of the subtraction results were obtained using ParaView 14 …”

Section: Methodsmentioning

confidence: 99%

High‐resolution Micro‐CT Myelography to Assess Spinal Subarachnoid Space Changes After Spinal Cord Injury in Rats

Zambrano-Rodríguez

Bolaños-Puchet

Reyes-Alva

et al. 2020

Journal of Neuroimaging

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BACKGROUND AND PURPOSE The spinal subarachnoid space (SSAS) is vital for neurologic function. Although SSAS alterations are known to occur after spinal cord injury (SCI), there is a lack of high‐resolution imaging studies of the SSAS after SCI in rodents. Therefore, the aim here was to assess changes in the SSAS of rats subjected to graded SCI, using high‐resolution micro‐CT myelography. METHODS Long‐Evans adult rats were subjected to mild or severe spinal cord contusion at T9. Imaging studies of SSAS features were carried out in injured rats at acute (day 1) and subacute (day 15) stages postinjury, as well as in control rats, using high‐resolution micro‐CT myelography with a contrast‐enhanced digital subtraction protocol. We studied a total of 33 rats randomly allocated into five experimental groups. Micro‐CT myelograms were assessed by expert observers using both qualitative and quantitative criteria. RESULTS Qualitative and quantitative analyses showed that SCI induces changes in the SSAS that vary as a function of both injury severity and time elapsed after injury. SSAS blockage was the main alteration detected. Moreover, the method used here allowed fine details to be observed in small animals, such as variations in the preferential pathways for contrast medium flow, neuroimaging nerve root enhancement, and leakage of contrast medium due to tearing of the dural sac. CONCLUSION Micro‐CT myelography provides high‐resolution images of changes in the SSAS after SCI in rats and is a useful tool for further experimental studies involving rat SCI in vivo.

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Section: Methodsmentioning

confidence: 99%