XRE family transcriptional regulator XtrSs modulates Streptococcus suis fitness under hydrogen peroxide stress

Liang, Song; Pan, Zihao; Yu, Yong; Yao, Huochun; Liu, Yongjie; Liu, Guangjin

doi:10.1007/s00203-022-02854-5

Cited by 7 publications

(5 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…XRE family transcriptional regulators are widespread in bacteria, and they are involved in the regulation of virulence, antibiotic synthesis and resistance, and stress response [ 43 , 44 ]. In S. suis , there are multiple XRE family transcriptional regulators, among which SrtR has been previously confirmed to regulate the virulence in a murine model and oxidative stress tolerance [ 45 ], and XtrSs was shown to be involved in response to hydrogen peroxide stress [ 46 ]. Moreover, XRE family transcriptional regulators were shown to participate in the direct regulation of virulence factors.…”

Section: Discussionmentioning

confidence: 99%

Screening for Virulence-Related Genes via a Transposon Mutant Library of Streptococcus suis Serotype 2 Using a Galleria mellonella Larvae Infection Model

Jia

Zhao

et al. 2022

Microorganisms

View full text Add to dashboard Cite

Streptococcus suis (S. suis) is a zoonotic bacterial pathogen causing lethal infections in pigs and humans. Identification of virulence-related genes (VRGs) is of great importance in understanding the pathobiology of a bacterial pathogen. To identify novel VRGs, a transposon (Tn) mutant library of S. suis strain SC19 was constructed in this study. The insertion sites of approximately 1700 mutants were identified by Tn-seq, which involved 417 different genes. A total of 32 attenuated strains were identified from the library by using a Galleria mellonella larvae infection model, and 30 novel VRGs were discovered, including transcription regulators, transporters, hypothetical proteins, etc. An isogenic deletion mutant of hxtR gene (ΔhxtR) and its complementary strain (CΔhxtR) were constructed, and their virulence was compared with the wild-type strain in G. mellonella larvae and mice, which showed that disruption of hxtR significantly attenuated the virulence. Moreover, the ΔhxtR strain displayed a reduced survival ability in whole blood, increased sensitivity to phagocytosis, increased chain length, and growth defect. Taken together, this study performed a high throughput screening for VRGs of S. suis using a G. mellonella larvae model and further characterized a novel critical virulence factor.

show abstract

Section: Discussionmentioning

confidence: 99%

Screening for Virulence-Related Genes via a Transposon Mutant Library of Streptococcus suis Serotype 2 Using a Galleria mellonella Larvae Infection Model

Jia

Zhao

et al. 2022

Microorganisms

View full text Add to dashboard Cite

show abstract

“…For example, Thioredoxins play a major role in bacterial response to oxidative stress by quenching singlet oxygen, scavenging hydroxyl radicals and donating hydrogen to peroxidases ( Chae et al, 1994 ; Das and Das, 2000 ; Zeller and Klug, 2006 ; Lu and Holmgren, 2014 ; Cheng et al, 2017 ). Members of the xenobiotic response element (XRE) family transcriptional regulators, among other functions, have been reported to enhance oxidative stress tolerance in different bacteria species such as Streptococcus suis and Corynebacterium glutamicum ( Hu et al, 2019 ; Si et al, 2020 ; Zhang et al, 2022 ). Flavodoxin family proteins were reported to enhance tolerance of plant growth promoting rhizobacteria, such as Pseudomonas fluorescens Aur6 and Ensiifer meliloti , to oxidative stress ( Coba de la Peña et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of NaCl stress on exoproteome profiles of Bacillus amyloliquefaciens EB2003A and Lactobacillus helveticus EL2006H

Naamala,

Subramanian,

Msimbira

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

Salt stress can affect survival, multiplication and ability of plant growth promoting microorganisms to enhance plant growth. Changes in a microbe’s proteome profile is one of the mechanisms employed by PGPM to enhance tolerance of salt stress. This study was focused on understanding changes in the exoproteome profile of Bacillus amyloliquefaciens EB2003A and Lactobacillus helveticus EL2006H when exposed to salt stress. The strains were cultured in 100 mL M13 (B. amyloliquefaciens) and 100 mL De man, Rogosa and Sharpe (MRS) (L. helveticus) media, supplemented with 200 and 0 mM NaCl (control), at pH 7.0. The strains were then incubated for 48 h (late exponential growth phase), at 120 rpm and 30 (B. amyloliquefaciens) and 37 (L. helveticus) °C. The microbial cultures were then centrifuged and filtered sterilized, to obtain cell free supernatants whose proteome profiles were studied using LC–MS/MS analysis and quantified using scaffold. Results of the study revealed that treatment with 200 mM NaCl negatively affected the quantity of identified proteins in comparison to the control, for both strains. There was upregulation and downregulation of some proteins, even up to 100%, which resulted in identification of proteins significantly unique between the control or 200 mM NaCl (p ≤ 0.05), for both microbial species. Proteins unique to 200 mM NaCl were mostly those involved in cell wall metabolism, substrate transport, oxidative stress tolerance, gene expression and DNA replication and repair. Some of the identified unique proteins have also been reported to enhance plant growth. In conclusion, based on the results of the work described here, PGPM alter their exoproteome profile when exposed to salt stress, potentially upregulating proteins that enhance their tolerance to this stress.

show abstract

“…Engineered bacteria BL21 containing XtrSs dimer -pET28a(+) were stored in our laboratory. Details of protein expression and purification methods referred to previous descriptions [ 15 ]. After LB agar with kanamycin resuscitated the BL21, individual bacterial clones were picked, and the bacterial culture was added to 1 mM IPTG in log phase and incubated at 16°C for 16 h. Subsequently, we purified soluble his-tagged XtrSs dimer using nickel columns (GE Healthcare).…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have shown that transcriptional factors, including SalK/SalR [ 11 ], CiaRH [ 12 ], and LuxS/AI-2 [ 13 ], etc., could help S. suis adapt to different host environments and promote its pathogenicity by regulating the transcription of genes related to virulence and metabolism [ 14 ]. Recently, we reported a novel XRE family transcriptional regulator, XtrSs, involved in bacterial adaptation to hydrogen peroxide stress in S. suis CZ130302 [ 15 ]. Therefore, further research on the transcriptional regulation of S. suis during infection can provide a deeper explanation of its pathogenic mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Streptococcal arginine deiminase system defences macrophage bactericidal effect mediated by XRE family protein XtrSs

Zhang,

Liang,

Zhang

et al. 2024

Virulence

Self Cite

View full text Add to dashboard Cite

The arginine deiminase system (ADS) has been identified in various bacteria and functions to supplement energy production and enhance biological adaptability. The current understanding of the regulatory mechanism of ADS and its effect on bacterial pathogenesis is still limited. Here, we found that the XRE family transcriptional regulator XtrSs negatively affected Streptococcus suis virulence and significantly repressed ADS transcription when the bacteria were incubated in blood. Electrophoretic mobility shift (EMSA) and lacZ fusion assays further showed that XtrSs directly bind to the promoter of ArgR, an acknowledged positive regulator of bacterial ADS, to repress ArgR transcription. Moreover, we provided compelling evidence that S. suis could utilize arginine via ADS to adapt to acid stress, while Δ xtrSs enhanced this acid resistance by upregulating the ADS operon. Moreover, whole ADS-knockout S. suis increased arginine and antimicrobial NO in the infected macrophage cells, decreased intracellular survival, and even caused significant attenuation of bacterial virulence in a mouse infection model, while Δ xtrSs consistently presented the opposite results. Our experiments identified a novel ADS regulatory mechanism in S. suis , whereby XtrSs regulated ADS to modulate NO content in macrophages, promoting S. suis intracellular survival. Meanwhile, our findings provide a new perspective on how Streptococci evade the host’s innate immune system.

show abstract

XRE family transcriptional regulator XtrSs modulates Streptococcus suis fitness under hydrogen peroxide stress

Cited by 7 publications

References 38 publications

Screening for Virulence-Related Genes via a Transposon Mutant Library of Streptococcus suis Serotype 2 Using a Galleria mellonella Larvae Infection Model

Screening for Virulence-Related Genes via a Transposon Mutant Library of Streptococcus suis Serotype 2 Using a Galleria mellonella Larvae Infection Model

Effect of NaCl stress on exoproteome profiles of Bacillus amyloliquefaciens EB2003A and Lactobacillus helveticus EL2006H

Streptococcal arginine deiminase system defences macrophage bactericidal effect mediated by XRE family protein XtrSs

Contact Info

Product

Resources

About