XRMA and ToF-SIMS Analysis of Normal and Hypomineralized Enamel

Melin, Lisa; Lundgren, Jesper; Malmberg, Per; Norén, Jörgen G.; Taube, Fabian; Cornell, David H.

doi:10.1017/s1431927615000033

Cited by 13 publications

(18 citation statements)

References 71 publications

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“…The dentin and enamel form distinct populations, similar for both tooth types, yet a slightly lower Mg/P ratio in hypoplastic enamel for similar Ca/P ratios. The elemental trends generally agree with the distributions found in previous studies …”

Section: Resultssupporting

confidence: 90%

Hyper‐spectral Raman imaging correlating chemical substitution and crystallinity in biogenic hydroxyapatite: Dentin and enamel in normal and hypoplastic human teeth

Wang

Anderson²,

Muller³

et al. 2018

J Raman Spectroscopy

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Micro‐Raman imaging and spectroscopy has become an established technique for the characterization of biogenic hydroxyapatite as, for example, the primary constituent of human teeth. However, few studies have yet gone beyond a qualitative analysis of the Raman response providing only limited insight into spatial heterogeneity of composition, structure, and degree of crystallinity. Here, we show how correlative electron microprobe and extended hyperspectral Raman imaging with high spatial and spectral resolution, with peak position and linewidth analysis, and from the μm to mm scale, provides insight into structural characteristics in dentin and enamel. From comparison of healthy and hypoplastic teeth as a representative tooth disease example, we determine variations in degree of crystallinity, both locally across the dentin–enamel junction, and with distinct long‐range spatial variations. We identify a correlation of spectral peak position and linewidth as a measure of crystal lattice disorder across tubules, dentin, dentin–enamel junction, and enamel. This correlative Raman imaging and analysis approach may help to provide a better understanding of apatite geochemistry and biomineralization.

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Section: Resultssupporting

confidence: 90%

Hyper‐spectral Raman imaging correlating chemical substitution and crystallinity in biogenic hydroxyapatite: Dentin and enamel in normal and hypoplastic human teeth

Wang

Anderson²,

Muller³

et al. 2018

J Raman Spectroscopy

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“…In contrast to a previous report, the magnesium content was lower in hypomineralized enamel than in normal enamel (27). A possible explanation for this could be differences in the matrix between hypomineralized and normal enamel caused by the higher amounts of organic material in the hypomineralized enamel, or a difference in concentration locally on the surface.…”

Section: Discussioncontrasting

confidence: 94%

“…The increased 13 C peak intensities for hypomineralized enamel are in good agreement with the ToF‐SIMS results and consistent with previous findings on a disrupted protein removal in enamel diagnosed with MIH . It is likely that the peaks in the 13 C cross‐polarization spectrum arose from proteins (such as amelogenins and phospholipids) or from enzymes (such as proteinases or collagen).…”

Section: Discussionsupporting

confidence: 91%

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Molecular insights into hypomineralized enamel

Malmberg

Norén

Bernin

2019

European J Oral Sciences

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Hypomineralized enamel may be found in connection with the condition molar incisor hypomineralization (MIH), which has a prevalence of around 15% in most parts of the world. Molar incisor hypomineralization is associated with extensive objective and subjective problems, such as hypersensitivity of the affected teeth, enamel breakdown, and problems with retention of restorations. The etiology behind MIH has not yet been elucidated, but a number of possible factors, which affect the same or different functions of ameloblasts during their different stages of maturation, have been suggested. The aim of this study was to utilize multi‐nuclear, solid‐state nuclear magnetic resonance (ss‐NMR) and time‐of‐flight secondary ion mass spectroscopy (ToF‐SIMS) to elucidate any differences, at a molecular level, between enamel powder prepared from normal, healthy teeth and enamel powder prepared from teeth diagnosed with MIH. 31P and 23Na ss‐NMR confirmed the presence of HPO42− and two different Na+ sites in hypomineralized enamel, suggesting a heterogeneous chemical composition. The content of organic components was higher in hypomineralized enamel, as shown by both 13C ss‐NMR and ToF‐SIMS, indicating the presence of higher numbers of proteins and phospholipids. The interplay between both is necessary for the formation and mineralization of enamel, which might be disturbed or halted in hypomineralized enamel.

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“…Inductive methods are powerful tools for analyses of, for example, patterns in data sets, and have been used for different applications (Klingberg et al 1999; Melin et al 2015). The outcome values in an inductive analysis have to be discrete; however, ingoing attributes can be numerical as well as discrete.…”

Section: Methodsmentioning

confidence: 99%

Behavioural characteristics in externalising children with low and elevated risk for dental caries

Staberg

Norén

Gahnberg

et al. 2016

Eur Arch Paediatr Dent

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AimTo compare two groups of children with externalising behaviour problems, having low and elevated caries risk, respectively. Those parameters were assessed in relation to behavioural characteristics and family structure, and to compare the caries risk assessment and gender differences in relation to children in general in the Region of Västra Götaland, Sweden.MethodsFamilies (228) with children, aged 10-13 years, participating in parent training programmes, were recruited. Parents provided information through questionnaires regarding parental knowledge and monitoring, family warmth and conflict and family structure. Children’s behavioural characteristics, based on the Strength and Difficulties Questionnaire and the Disruptive Behaviour Disorder rating scale, were used as outcome. Data about caries risk assessment were obtained from dental records.ResultsChildren in the elevated caries risk group showed higher mean values for conduct problems as well as impulsivity. Parents of the children in the elevated caries risk group reported more parental solicitation and less family conflicts. Children with an elevated caries risk lived more often in households with more than two children and had more often a father from a non-Nordic country.ConclusionThere were statistically significant more children with an elevated caries risk in the study group compared to children in general in the Region of Västra Götaland, both totally and within gender. Differences were observed with regard to behavioural characteristics in externalising children with an elevated risk for caries. Increased knowledge regarding behavioural characteristics in externalising children is an important parameter to be considered in caries risk assessment.

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XRMA and ToF-SIMS Analysis of Normal and Hypomineralized Enamel

Cited by 13 publications

References 71 publications

Hyper‐spectral Raman imaging correlating chemical substitution and crystallinity in biogenic hydroxyapatite: Dentin and enamel in normal and hypoplastic human teeth

Hyper‐spectral Raman imaging correlating chemical substitution and crystallinity in biogenic hydroxyapatite: Dentin and enamel in normal and hypoplastic human teeth

Molecular insights into hypomineralized enamel

Behavioural characteristics in externalising children with low and elevated risk for dental caries

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