Let G be a metacyclic p-group, and let Z(G) be its center. The non-commuting graph Γ G of a metacyclic pgroup G is defined as the graph whose vertex set is G−Z(G), and two distinct vertices x and y are connected by an edge if and only if the commutator of x and y is not the identity. In this paper, we give some graph theoretical properties of the non-commuting graph Γ G . Particularly, we investigate planarity, completeness, clique number and chromatic number of such graph. Also, we prove that if G 1 and G 2 are isoclinic metacyclic p-groups, then their associated graphs are isomorphic.