2000
DOI: 10.1007/s007920070028
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Xylanase from the psychrophilic yeast Cryptococcus adeliae

Abstract: A xylanase belonging to family 10 is produced by Cryptococcus adeliae, an Antarctic yeast that exhibits optimal growth at low temperature. The mature glycosylated xylanase secreted by C. adeliae is composed of 338 amino acid residues and 26 +/- 3 osidic residues, and shares 84% identity with its mesophilic counterpart from C. albidus. The xylanase from C. adeliae is less thermostable than its mesophilic homologue when the residual activities are compared, and this difference was confirmed by differential scann… Show more

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Cited by 111 publications
(57 citation statements)
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“…The effect of temperature on chemical reactions is basically described by the Arrhenius equation: k = Ae ϪEa/RT , where k is the rate constant, A is the pre-exponential factor that depends on the reaction, enzyme with enhanced catalytic efficiency k cat /K m . For extracellular enzymes that work at saturating substrate concentrations, adaptation consists mainly of increasing k cat Narinx et al 1997;Petrescu et al 2000). Alternatively, for secreted marine enzymes or intracellular enzymes that could face low substrate concentrations, a decrease in K m (Michaelis-Menten constant) providing a higher substrate affinity could be useful (Kim et al 1999;Bentahir et al 2000;Georlette et al 2000;Hoyoux et al 2001).…”
Section: The Low Temperature Challengementioning
confidence: 99%
“…The effect of temperature on chemical reactions is basically described by the Arrhenius equation: k = Ae ϪEa/RT , where k is the rate constant, A is the pre-exponential factor that depends on the reaction, enzyme with enhanced catalytic efficiency k cat /K m . For extracellular enzymes that work at saturating substrate concentrations, adaptation consists mainly of increasing k cat Narinx et al 1997;Petrescu et al 2000). Alternatively, for secreted marine enzymes or intracellular enzymes that could face low substrate concentrations, a decrease in K m (Michaelis-Menten constant) providing a higher substrate affinity could be useful (Kim et al 1999;Bentahir et al 2000;Georlette et al 2000;Hoyoux et al 2001).…”
Section: The Low Temperature Challengementioning
confidence: 99%
“…Psychrophiles were first referred to as cold-adapted bacteria [12]. However, if a psychrophile is defined as an organism living permanently at temperatures close to the freezing point of water in thermal equilibrium with the medium, such a definition includes de facto a large range of species: yeast [13][14][15][16], algae [17][18][19], marine invertebrates and insects or polar fish, which are the largest psychrophiles [20,21]. These examples underline that psychrophiles are numerous, taxonomically diverse and have a widespread distribution.…”
mentioning
confidence: 99%
“…In comparison to thermophiles, relatively few psychrophilic xylanolytic organisms have been described. The psychrophilic xylanolytic organisms that have been described include the anaerobic prokaryote Clostridium vincentii (Mounfort et al, 1997) ; the aerobic prokaryote Hymenobacter roseosalivarius (Hirsch et al, 1998) and the eukaryotes Cryptococcus TAE85 (Gerday et al, 1997) and Cryptococcus adeliae (Petrescu et al, 2000). The phenotypic and genotypic features of isolate A2i T suggest that it is a member of the genus Flavobacterium.…”
Section: Discussionmentioning
confidence: 99%