Xylanase inhibition by the derivatives of lignocellulosic material

Hidayatullah, Ibnu Maulana; Setiadi, Tjandra; Kresnowati, Made Tri Ari Penia; Boopathy, Ramaraj

doi:10.1016/j.biortech.2020.122740

Cited by 27 publications

(18 citation statements)

References 17 publications

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“…Furthermore, extending the hydrothermal pretreatment time to 180 min at 170 • C lowered the xylose recovery of beechwood [25]. A longer pretreatment time leads to the formation of furfural, soluble lignin, and insoluble lignin, which was reported to inhibit the enzymatic hydrolysis process [25,48]. Compared to previous research using grapevine followed by acid hydrolysis, where 60% of xylan was recovered in the liquid pretreated biomass [25].…”

Section: Discussionmentioning

confidence: 84%

Optimization of Xylose Recovery in Oil Palm Empty Fruit Bunches for Xylitol Production

et al. 2020

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The hardest obstacle to make use of lignocellulosic biomass by using green technology is the existence of lignin. It can hinder enzyme reactions with cellulose or hemicellulose as a substrate. Oil palm empty fruit bunches (OPEFBs) consist of hemicellulose with xylan as the main component. Xylitol production via fermentation could use this xylan since it can be converted into xylose. Several pretreatment processes were explored to increase sugar recovery from lignocellulosic biomass. Considering that hemicellulose is more susceptible to heat than cellulose, the hydrothermal process was applied to OPEFB before it was hydrolyzed enzymatically. The purpose of this study was to investigate the effect of temperature, solid loading, and pretreatment time on the OPEFB hydrothermal process. The xylose concentration in OPEFB hydrolysate was analyzed using high-performance liquid chromatography (HPLC). The results indicated that temperature was more important than pretreatment time and solid loading for OPEFB sugar recovery. The optimum temperature, solid loading, and pretreatment time for maximum xylose recovery from pretreated OPEFB were 165 °C, 7%, and 60 min, respectively, giving a xylose recovery of 0.061 g/g of pretreated OPEFB (35% of OPEFB xylan was recovered).

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Section: Discussionmentioning

confidence: 84%

Optimization of Xylose Recovery in Oil Palm Empty Fruit Bunches for Xylitol Production

et al. 2020

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“…Xylanase may destroy the original network structure of lignocellulose, thereby increasing the degree of swelling and porosity. Xylanase, as an auxiliary enzyme, damages the physical structure of lignocellulose to promote the penetration of cellulase into the microfibrous pores of cellulose and easier binding to cellulose, thereby accelerating substrate hydrolysis and improving reducing sugar yield [ 38 ]. The synergistic action of xylanase is a reliable strategy to overcome the obstacles in the saccharification reaction, and to eventually ensure the maximum reducing sugar yield from lignocellulosic substrates with the least enzyme titer.…”

Section: Resultsmentioning

confidence: 99%

Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries

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Zha

et al. 2021

Biotechnol Biofuels

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Background Xylanase, an important accessory enzyme that acts in synergy with cellulase, is widely used to degrade lignocellulosic biomass. Thermostable enzymes with good catalytic activity at lower temperatures have great potential for future applications in the feed and fuel industries, which have distinct demands; however, the potential of the enzymes is yet to be researched. Results In this study, a structure-based semi-rational design strategy was applied to enhance the low-temperature catalytic performance of Bispora sp. MEY-1 XYL10C_∆N wild-type (WT). Screening and comparisons were performed for the WT and mutant strains. Compared to the WT, the mutant M53S/F54L/N207G exhibited higher specific activity (2.9-fold; 2090 vs. 710 U/mg) and catalytic efficiency (2.8-fold; 1530 vs. 550 mL/s mg) at 40 °C, and also showed higher thermostability (the melting temperature and temperature of 50% activity loss after 30 min treatment increased by 7.7 °C and 3.5 °C, respectively). Compared with the cellulase-only treatment, combined treatment with M53S/F54L/N207G and cellulase increased the reducing sugar contents from corn stalk, wheat bran, and corn cob by 1.6-, 1.2-, and 1.4-folds, with 1.9, 1.2, and 1.6 as the highest degrees of synergy, respectively. Conclusions This study provides useful insights into the underlying mechanism and methods of xylanase modification for industrial utilization. We identified loop2 as a key functional area affecting the low-temperature catalytic efficiency of GH10 xylanase. The thermostable mutant M53S/F54L/N207G was selected for the highest low-temperature catalytic efficiency and reducing sugar yield in synergy with cellulase in the degradation of different types of lignocellulosic biomass. Graphic Abstract

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“…It is important to optimize all factors involved so that the different steps, with different optimal conditions, can be carried out at their optimums. Drawbacks cited in SSF processes are (a) the disparity in suitable temperatures being 50 • C for enzymatic hydrolysis but 30 • C for yeast fermentation (described above), (b) ethanol being an inhibitor for common xylanases in processes where both hexoses and pentoses are to be fermented [149], or (c) LPMOs causing competition of available oxygen between LPMOs and yeast [150]. Strategies such as the use of thermotolerant microorganisms, the discovery of new xylanolytic enzymes, and/or the use of H 2 O 2 as an oxidizing agent have been studied to overcome these challenges.…”

Section: Improving Microbial Performance For Ethanol Productionmentioning

confidence: 99%

Advanced Bioethanol Production: From Novel Raw Materials to Integrated Biorefineries

et al. 2021

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The production of so-called advanced bioethanol offers several advantages compared to traditional bioethanol production processes in terms of sustainability criteria. This includes, for instance, the use of nonfood crops or residual biomass as raw material and a higher potential for reducing greenhouse gas emissions. The present review focuses on the recent progress related to the production of advanced bioethanol, (i) highlighting current results from using novel biomass sources such as the organic fraction of municipal solid waste and certain industrial residues (e.g., residues from the paper, food, and beverage industries); (ii) describing new developments in pretreatment technologies for the fractionation and conversion of lignocellulosic biomass, such as the bioextrusion process or the use of novel ionic liquids; (iii) listing the use of new enzyme catalysts and microbial strains during saccharification and fermentation processes. Furthermore, the most promising biorefinery approaches that will contribute to the cost-competitiveness of advanced bioethanol production processes are also discussed, focusing on innovative technologies and applications that can contribute to achieve a more sustainable and effective utilization of all biomass fractions. Special attention is given to integrated strategies such as lignocellulose-based biorefineries for the simultaneous production of bioethanol and other high added value bioproducts.

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Xylanase inhibition by the derivatives of lignocellulosic material

Cited by 27 publications

References 17 publications

Optimization of Xylose Recovery in Oil Palm Empty Fruit Bunches for Xylitol Production

Optimization of Xylose Recovery in Oil Palm Empty Fruit Bunches for Xylitol Production

Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries

Advanced Bioethanol Production: From Novel Raw Materials to Integrated Biorefineries

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