2024
DOI: 10.3390/app14051726
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Xylene versus Isopropanol for Paraffin Wax Processing of Lung Tissue

Qi Wang,
Runchuan Gu,
Franziska Olm
et al.

Abstract: The microscopic observation of lung tissue is challenging due to its fragile nature. Xylene and isopropanol are common tissue-clearing reagents used before paraffin embedding, yet no studies have compared these two reagents in lung tissue processing. Due to the well-known health risks xylene could introduce to operators, as well as its environmental hazards, it has long been desired that a less harmful alternative to xylene with the same staining effects be introduced. Thus, we systematically assessed the effi… Show more

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