XYLOOLIGOSACCHARIDES PRODUCTION FROM OIL PALM FROND BY Trichoderma longibrachiatum XYLANASE

Abstract

Oil palm fronds containing rich hemicellulose are low cost resources that could be potentially converted into valuable products such as xylooligosaccharides. The main objective of this study was to investigate the production of xylooligosaccharides from OPF hemicelluloses using Trichoderma longibrachiatum xylanase. The OPF hemicellulose extracted by alkaline extraction was hydrolysed by xylanase at pH 4.6, temperature 40 °C, hemicellulose substrate concentration 2 % (w/v) and enzyme concentration 2 U/ml for different period of time from 0 to 48 hours to produce xylooligosaccharides. The hydrolysate obtained from enzymatic hydrolysis was further purified through ultrafiltration using 10 kDa molecular weight cut off membranes. The highest total of xylobiose and xylotriose was found to be 21.91 mg/mL and obtained at 8 hours of hydrolysis time. After ultrafiltration step, xylooligosaccharides mixture were obtained in the permeate and retentate. The highest xylobiose (56.64g/100g) and xylotriose (45.80g/100g) were found in retentate and permeate, respectively.