Xylopentose production from crop residue employing xylanase enzyme

Kaur, Pritam; Khatri, Madhu; Singh, Gursharan; Selvaraj, Manickam; Assiri, Mohammed A.; Rokhum, Samuel Lalthazuala; Arya, Shailendra Kumar; Jones, Sumathi; Greff, Babett; Chang, Soon Woong; Ravindran, Balasubramani; Awasthi, Mukesh Kumar

doi:10.1016/j.biortech.2022.128572

Cited by 4 publications

(1 citation statement)

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“…The maximum xylanase production was through sugarcane bagasse (472.03 U/mL) under SSF conditions. Accordingly, enhanced xylanase production employing multiple agricultural substrates, as well as high levels of enzyme production at lower substrate-to-moisture ratios, have previously been reported (Ketsakhon et al 2022 ; Kaur et al 2023 ). Sugarcane bagasse is an inexpensive byproduct with high xylan content; therefore, it is one of the most utilizable components of media for xylanase production (Alokika and Singh 2020 ; Moran-Aguilar et al 2021 ).…”

Section: Resultsmentioning

confidence: 92%

Valorization of lignocellulosic wastes for sustainable xylanase production from locally isolated Bacillus subtilis exploited for xylooligosaccharides’ production with potential antimicrobial activity

El-Gendi,

Badawy,

Bakhiet

et al. 2023

Arch Microbiol

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The worldwide availability of lignocellulosic wastes represents a serious environmental challenge with potential opportunities. Xylanases are crucial in lignocellulosic bio-hydrolysis, but the low enzyme productivity and stability are still challenges. In the current study, Bacillus subtilis (coded ARSE2) revealed potent xylanase activity among other local isolates. The enzyme production optimization revealed that maximum enzyme production (490.58 U/mL) was achieved with 1% xylan, 1.4% peptone, and 5% NaCl at 30 °C and pH 9. Furthermore, several lignocellulosic wastes were exploited for sustainable xylanase production, where sugarcane bagasse (16%) under solid-state fermentation and woody sawdust (2%) under submerged fermentation supported the maximum enzyme titer of about 472.03 and 485.7 U/mL, respectively. The partially purified enzyme revealed two protein bands at 42 and 30 kDa. The partially purified enzyme revealed remarkable enzyme activity and stability at 50–60 °C and pH 8–9. The enzyme also revealed significant stability toward tween-80, urea, DTT, and EDTA with Vmax and Km values of 1481.5 U/mL and 0.187 mM, respectively. Additionally, the purified xylanase was applied for xylooligosaccharides production, which revealed significant antimicrobial activity toward Staphylococcus aureus with lower activity against Escherichia coli. Hence, the locally isolated Bacillus subtilis ARSE2 could fulfill the xylanase production requirements in terms of economic production at a high titer with promising enzyme characteristics. Additionally, the resultant xylooligosaccharides revealed a promising antimicrobial potential, which paves the way for other medical applications.

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