Xylose and xylose/glucose co-fermentation by recombinant Saccharomyces cerevisiae strains expressing individual hexose transporters

Gonçalves, Davi L.; Matsushika, Akinori; Sales, Belisa Bordin de; Goshima, Tetsuya; Bon, Elba P. S.; Stambuk, Boris U.

doi:10.1016/j.enzmictec.2014.05.003

Cited by 72 publications

(55 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite efforts to develop pentose-utilizing strains, pentose consumption by recombinant S. cerevisiae is still slower than glucose consumption (Oreb et al 2012). To tackle this obstacle, approaches including overexpression of transporter for pentose uptake (Farwick et al 2014;Goncalves et al 2014;Young et al 2014), evolutionary engineering of strains (Lee et al 2014;Smith et al 2014), and optimization of gene expression related to pentose metabolism (Cao et al 2014) have been employed, although to our best knowledge, none of the resultant strains exhibit efficient utilization of xylose at a level comparable to that of glucose. Another technological challenge for industrial production of cellulosic ethanol is the formation of inhibitory compounds, such as furans, aromatic compounds, and weak organic acids during the thermochemical treatment of lignocellulosic biomass, a pretreatment process that is required to enhance accessibility of biomass to saccharification enzymes.…”

Section: Introductionmentioning

confidence: 98%

Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum

Jojima

Noburyu

Sasaki

et al. 2014

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Recombinant Corynebacterium glutamicum harboring genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) can produce ethanol under oxygen deprivation. We investigated the effects of elevating the expression levels of glycolytic genes, as well as pdc and adhB, on ethanol production. Overexpression of four glycolytic genes (pgi, pfkA, gapA, and pyk) in C. glutamicum significantly increased the rate of ethanol production. Overexpression of tpi, encoding triosephosphate isomerase, further enhanced productivity. Elevated expression of pdc and adhB increased ethanol yield, but not the rate of production. Fed-batch fermentation using an optimized strain resulted in ethanol production of 119 g/L from 245 g/L glucose with a yield of 95% of the theoretical maximum. Further metabolic engineering, including integration of the genes for xylose and arabinose metabolism, enabled consumption of glucose, xylose, and arabinose, and ethanol production (83 g/L) at a yield of 90 %. This study demonstrated that C. glutamicum has significant potential for the production of cellulosic ethanol.

show abstract

Section: Introductionmentioning

confidence: 98%

Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum

Jojima

Noburyu

Sasaki

et al. 2014

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Xylose consumption decreased slightly based on the ratio with the increase of glucose concentration (Fig. 4), which was most likely due to the competition for transporter by high concentration of glucose (Gonçalves et al 2014).…”

Section: Xylose-utilizing Yeast Constructionmentioning

confidence: 95%

“…Strategies to avoid glucose repression were developed based on the cellobiose transporter (Saitoh et al 2010;Ha et al 2011;Zha et al 2013). Hexose transporters have been analyzed for xylose and glucose specificity, and the results revealed that the moderately high-affinity permease allows xylose uptake at the same rate as that of glucose (Gonçalves et al 2014). In Escherichia coli, simultaneous aerobic utilization of glucose and xylose was achieved by metabolic coupling with the help of a rational bilevel optimization algorithm, although the engineered E. coli exhibited a slower glucose consumption rate compared to the native strain (Gawand et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Deletion of d-ribulose-5-phosphate 3-epimerase (RPE1) induces simultaneous utilization of xylose and glucose in xylose-utilizing Saccharomyces cerevisiae

Shen

Song

et al. 2014

Biotechnol Lett

View full text Add to dashboard Cite

Simultaneous co-utilization of xylose and glucose is a key issue in engineering microbes for cellulosic ethanol production. We coupled xylose utilization with glucose metabolism by deletion of D-ribulose-5-phosphate 3-epimerase (RPE1) through pentose phosphate pathway flux. Simultaneous utilization of xylose and glucose then occurred in the engineered Saccharomyces cerevisiae strain with the xylose utilization pathway. Xylose consumption occurred at the beginning of glucose consumption by the engineered yeast without RPE1 in a mixed sugar fermentation. About 3.2 g xylose l(-1) was utilized simultaneously with consumption of 40.2 g glucose l(-1) under O2-limited conditions. In addition, an approximate ratio (~1:10) for xylose and glucose consumption was observed in the fermentation with different sugar concentration by the engineered strain without RPE1. Simultaneous utilization of xylose is realized by the coupling of glucose metabolism and xylose utilization through RPE1 deletion in xylose-utilizing S. cerevisiae.

show abstract

“…Genes for XR and XDH from S. stipitis and the xylulose kinase gene (Fig. 4) from S. cerevisiae were introduced into the INVSc1 strain 35) , shochu and bakery yeast 36) , sake yeast 37) , and the strain devoid of hexose transporters for research of xylose uptake 38) . However, the recombinant strains with XR and XDH accumulated quite high concentrations of xylitol, which reduced the ethanol yield from xylose.…”

Section: Development Of Xylose-fermenting Yeast Strainsmentioning

confidence: 99%

Enzymatic Saccharification and Fermentation Technology for Ethanol Production from Woody Biomass

Yano

2015

J. Jpn. Petrol. Inst.

View full text Add to dashboard Cite

Fuel ethanol production from biomass is promising technology for alleviation of global warming and reduction of fossil fuel use. In particular, production from non-food resources such as woody biomass is required. However, there are several technological problems such as development of efficient pretreatment technology, reduction of enzyme costs, and inability of conventional yeast to ferment xylose. Our research center has been conducting research to solve these problems and developed our own technology for enhancement of enzyme productivity and development of xylose-fermenting yeast strains. Our technology was effective in bench scale ethanol production experiments from eucalyptus wood using an integrated process from pretreatment to fermentation with high solid saccharification and glucose/xylose co-fermentation using a xylose-fermenting yeast strain. Our results show that ethanol production from woody biomass is feasible.

show abstract

Xylose and xylose/glucose co-fermentation by recombinant Saccharomyces cerevisiae strains expressing individual hexose transporters

Cited by 72 publications

References 81 publications

Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum

Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum

Deletion of d-ribulose-5-phosphate 3-epimerase (RPE1) induces simultaneous utilization of xylose and glucose in xylose-utilizing Saccharomyces cerevisiae

Enzymatic Saccharification and Fermentation Technology for Ethanol Production from Woody Biomass

Contact Info

Product

Resources

About