2024
DOI: 10.1101/2024.05.09.593338
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Y chromosome shredding inAnopheles gambiae: insight into the cellular dynamics of a novel synthetic sex ratio distorter

Matteo Vitale,
Nace Kranjc,
Jessica Leigh
et al.

Abstract: Despite efforts to explore the genome of the malaria vector Anopheles gambiae, the Y chromosome of this species remains enigmatic. The large number of repetitive and heterochromatic DNA sequences makes the Y chromosome exceptionally difficult to fully assemble, hampering the progress of gene editing techniques and functional studies for this chromosome. In this study, we made use of a bioinformatic platform to identify Y-specific repetitive DNA sequences that served as a target site for a CRISPR/Cas9 system. T… Show more

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