Y-STR DNA amplification as biological evidence in sexually assaulted female victims with no cytological detection of spermatozoa

Modified Practical Approach in Repeated DNA Studies of Biological Evidence of Sexual Assault

“…it is well known that, if no sperm is found in the vaginal or anal content of those who have suffered a sexual assault, this does not rule out the possibility of them having suffered such an offence (39) or a manual penetration. it is in such cases that the studies of collected evidence focus on the search for epithelial cell biological material deposited on it (24).…”

“…Results of studies on this topic show that the analyses can prove successful if the samples have been taken within 48 hours to 8 days after a sexual offense, and that, within this time span, sperm can be found in material from oral and anal swabs (26,39). Some authors report successful DnA typing of smears fixed with ethanol, stained with different techniques and fixed with conditioner resins (8).…”

Evaluation of Y-STR analyses of sperm cell negative vaginal samples

“…Baecchi's staining [1] or the Sperm Hy-Liter immuno-fluorescence method [2]. Due to several factors, sperm cells are not detected in some cases of sexual assault [3][4][5]. Some sperm cell negative sexual assault samples contain traces of other male cells [4,5].…”

“…Due to several factors, sperm cells are not detected in some cases of sexual assault [3][4][5]. Some sperm cell negative sexual assault samples contain traces of other male cells [4,5]. It has previously been shown that Y-STR profiles can be obtained from sexual assault samples with no microscopic evidence of sperm cells [e.g.…”

“…It has previously been shown that Y-STR profiles can be obtained from sexual assault samples with no microscopic evidence of sperm cells [e.g. 4,5].…”