Y2SK2 and SK3 type dehydrins from Agapanthus praecox can improve plant stress tolerance and act as multifunctional protectants

Zhou, Yang; Sheng, Jiangyuan; Lv, Ke; Ren, Li; Zhang, Di

doi:10.1016/j.plantsci.2019.03.012

Cited by 31 publications

(27 citation statements)

References 57 publications

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“…Two DHN genes from Agapanthus praecox, ApY2SK2 and ApSK3, displayed essential protective effects during complicated stresses [121]. The overexpression of ApY2SK2 and ApSK3 in Arabidopsis thaliana led to reduction in damage to the plasma membrane and ROS levels and caused higher antioxidant activity and photosynthesis capability during osmotic stresses in comparison to the wild-type species [121]. In a study, a YSK2-type DHN from Sorghum bicolor, SbDhn1, generated a high level of transcript accumulation when exposed to osmotic stress.…”

Section: Expression Of Group II Lea Genes Under Osmotic Stressmentioning

confidence: 99%

Plant Group II LEA Proteins: Intrinsically Disordered Structure for Multiple Functions in Response to Environmental Stresses

et al. 2021

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In response to various environmental stresses, plants have evolved a wide range of defense mechanisms, resulting in the overexpression of a series of stress-responsive genes. Among them, there is certain set of genes that encode for intrinsically disordered proteins (IDPs) that repair and protect the plants from damage caused by environmental stresses. Group II LEA (late embryogenesis abundant) proteins compose the most abundant and characterized group of IDPs; they accumulate in the late stages of seed development and are expressed in response to dehydration, salinity, low temperature, or abscisic acid (ABA) treatment. The physiological and biochemical characterization of group II LEA proteins has been carried out in a number of investigations because of their vital roles in protecting the integrity of biomolecules by preventing the crystallization of cellular components prior to multiple stresses. This review describes the distribution, structural architecture, and genomic diversification of group II LEA proteins, with some recent investigations on their regulation and molecular expression under various abiotic stresses. Novel aspects of group II LEA proteins in Phoenix dactylifera and in orthodox seeds are also presented. Genome-wide association studies (GWAS) indicated a ubiquitous distribution and expression of group II LEA genes in different plant cells. In vitro experimental evidence from biochemical assays has suggested that group II LEA proteins perform heterogenous functions in response to extreme stresses. Various investigations have indicated the participation of group II LEA proteins in the plant stress tolerance mechanism, spotlighting the molecular aspects of group II LEA genes and their potential role in biotechnological strategies to increase plants’ survival in adverse environments.

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Section: Expression Of Group II Lea Genes Under Osmotic Stressmentioning

confidence: 99%

Plant Group II LEA Proteins: Intrinsically Disordered Structure for Multiple Functions in Response to Environmental Stresses

et al. 2021

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“…Studies had showed that the formation of metal−glyphosate complexes could affect the herbicidal effect of glyphosate [58]. Yang et al uncovered that ApY2SK2 and ApSK3 dehydrin genes could enhance plant stress tolerance probably by regulating ROS metabolism and metal ions binding [59]. Hence, we speculate that MSTRG.244.1/ MSTRG.16577.1osa-miR156-SPL12 might take part in the responses of rice to glyphosate stress probably by regulating transcription and metal ions binding.…”

Section: Discussionmentioning

confidence: 75%

Identification and integrated analysis of glyphosate stress-responsive microRNAs, lncRNAs, and mRNAs in rice using genome-wide high-throughput sequencing

Zhai

Zhu

et al. 2020

BMC Genomics

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Background: Glyphosate has become the most widely used herbicide in the world. Therefore, the development of new varieties of glyphosate-tolerant crops is a research focus of seed companies and researchers. The glyphosate stress-responsive genes were used for the development of genetically modified crops, while only the EPSPS gene has been used currently in the study on glyphosate-tolerance in rice. Therefore, it is essential and crucial to intensify the exploration of glyphosate stress-responsive genes, to not only acquire other glyphosate stress-responsive genes with clean intellectual property rights but also obtain non-transgenic glyphosate-tolerant rice varieties. This study is expected to elucidate the responses of miRNAs, lncRNAs, and mRNAs to glyphosate applications and the potential regulatory mechanisms in response to glyphosate stress in rice. Results: Leaves of the non-transgenic glyphosate-tolerant germplasm CA21 sprayed with 2 mg·ml − 1 glyphosate (GLY) and CA21 plants with no spray (CK) were collected for high-throughput sequencing analysis. A total of 1197 DEGs, 131 DELs, and 52 DEMs were identified in the GLY samples in relation to CK samples. Genes were significantly enriched for various biological processes involved in detoxification of plant response to stress. A total of 385 known miRNAs from 59 miRNA families and 94 novel miRNAs were identified. Degradome analysis led to the identification of 32 target genes, of which, the squamosa promoter-binding-like protein 12 (SPL12) was identified as a target of osa-miR156a_L + 1. The lncRNA-miRNA-mRNA regulatory network consisted of osa-miR156a_L + 1, two transcripts of SPL12 (LOC_Os06g49010.3 and LOC_Os06g49010.5), and 13 lncRNAs (e.g., MSTRG.244.1 and MSTRG.16577.1). Conclusion: Large-scale expression changes in coding and noncoding RNA were observed in rice mainly due to its response to glyphosate. SPL12, osa-miR156, and lncRNAs (e.g., MSTRG.244.1 and MSTRG.16577.1) could be a novel ceRNA mechanism in response to glyphosate in rice by regulating transcription and metal ions binding. These findings provide a theoretical basis for breeding glyphosate-tolerant rice varieties and for further research on the biogenesis of glyphosate-tolerance in rice.

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“…Detection of physiological indices H 2 O 2 levels, O 2 inhabitation and OH• generation activities, MDA, SOD, CAT and POD activities, AsA and GSH contents were tested using related biological assay kits (Nanjing Jiancheng Bioengineering Institute, China) following the manufacturer's instructions with some modi cations as according to Yang et al [81].…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Single-wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus praecox Embryogenic Callus

Ren

Deng

Chu

et al. 2020

Preprint

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Background: Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue in the cryobiology field.Results: This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. The SWCNTs entered EC at the step of dehydration, and mainly located around the cell wall and in the vesicles, and most of SWCNTs moved out of EC during dilution step. Combination with physiological index and gene quantitative expression results, SWCNTs affect ROS signal transduction and antioxidant system response during plant cryopreservation. The EC treated by SWCNTs had higher antioxidant levels, like POD, CAT and GSH than the control group EC. EC mainly depended on AsA-GSH and GPX cycle to scavenge H2O2 in the control cryopreservation, but depended on CAT in the SWCNTs-added cryopreservation which lead to low levels of H2O2 and MDA. Elevated antioxidant level in dehydration by adding SWCNTs enhanced cells resistance to injury during cryopreservation. The ROS signals of EC were balanced and stable in the SWCNTs-added cryopreservation.Conclusions: SWCNTs regulated oxidative stress responses of EC during the process, and controlled oxidative damages by the maintenance of ROS homeostasis to achieve high survival rate after cryopreservation. This study is the first to systematically describe the role of carbon nanomaterial in the regulation of plant oxidative stress response, and provided a novel insight into the application of nanomaterials in the field of cryobiology.

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Y2SK2 and SK3 type dehydrins from Agapanthus praecox can improve plant stress tolerance and act as multifunctional protectants

Cited by 31 publications

References 57 publications

Plant Group II LEA Proteins: Intrinsically Disordered Structure for Multiple Functions in Response to Environmental Stresses

Plant Group II LEA Proteins: Intrinsically Disordered Structure for Multiple Functions in Response to Environmental Stresses

Identification and integrated analysis of glyphosate stress-responsive microRNAs, lncRNAs, and mRNAs in rice using genome-wide high-throughput sequencing

Single-wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus praecox Embryogenic Callus

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