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Abstract

Physical activity creates an imbalance between the production of free radicals and the body's antioxidant defense system, known as oxidative stress. Physical activity increases the need for oxygen, improving free radical products. Thus, causing increased levels of malondialdehyde (MDA), glutathione (GLU), and superoxide dismutase (SOD) can be overcome by consuming honey that contains antioxidants. This study aimed to determine the effect of honey administration on the physical activity of MDA, GLU, and SOD blood levels in male Wistar rats. Rats were then divided into 5 groups: healthy control, negative control, and M1 to 3 (giving 2.0, 2.5, and 3.0 g/kg BW (gram per kilogram body weight) of honey twice weekly for 8 weeks). High-intensity swimming exercises were performed for 1 h/day, 5 days/week for 8 weeks. According to the manual kit, the blood levels of MDA, GLU, and SOD were carried out by the ELISA method. Administration of 3 g/kg BW of honey significantly decreased MDA blood level (49.18 ± 0.74 ng/mL) and increased SOD blood level (132.43±10.30 pg/mL) compared to all groups (p < 0.05). The blood level of GLU significantly increased in all honey compared to the negative control, but no significant (p > 0.05) difference existed between honey groups. It may be possible that honey supplementation following physical exercise would effectively attenuate the antioxidant status.