Yamadazyma insecticola f.a., sp. nov. and Yamadazyma epiphylla f.a., sp. nov., two novel yeast species

Abstract

Two yeast strains representing two novel yeast species were isolated from frass of an unidentified insect (ST-78 T) and the external surfaces of rice leaves (YE170 T) collected in Thailand. The two strains were genetically, morphologically and phenotypically distinct from recognized species and were found to represent two novel species of the genus Yamadazyma although formation of ascospores was not observed. In terms of pairwise sequence similarity of the D1/D2 region of the large subunit rRNA gene, the closest relative of strain ST-78 T was Candida lessepsii CBS 9941 T but with 3.8 % nucleotide substitutions, while the closest relative of strain YE170 T was strain ST-78 T but with 4.3 % nucleotide substitutions. Analysis of the sequences of the internal transcribed spacer 1 and 2 (ITS1-2) regions revealed that strain ST-78 differed from C. lessepsii CBS 9941 T by 8.8 % nucleotide substitutions and from strain YE170 T by 9.4 % nucleotide substitutions. The result of pairwise sequence similarity of the D1/D2 and ITS1-2 regions together with phylogenetic analysis indicated that strains ST-78 T and YE170 T represented two novel species within the Yamadazyma clade. The names Yamadazyma insecticola f.a., sp. nov. (type strain ST-78 T 5BCC 8314 T 5NBRC 110421 T 5CBS 13382 T ; MycoBank no. MB810546) and Yamadazyma epiphylla f.a., sp. nov. (type strain YE170 T 5BCC 63466 T 5NBRC 110423 T 5CBS 13384 T ; MycoBank no. MB810547) are proposed for the two novel yeast species.

Yamadazyma endophytica f.a. sp. nov., an ascomycetous yeast species isolated from leaf tissue

“…In addition, several species in this clade have been described from the strains associated with insects Lachance et al, 2011;Jindamorakot et al, 2015) and plant materials (Nisiotou et al, 2010;Lachance et al, 2011;Kaewwichian et al, 2013b;Junyapate et al, 2014;Jindamorakot et al, 2015;Lopes et al, 2015;Wang et al, 2015). In this study, strain DMKU-CE23 T was isolated from corn leaf tissue.…”

“…endophytica within plant, endophytic, pertaining to the original isolation from plant tissues). Jindamorakot et al (2015). +, positive; w, weakly positive; s, slow; l, latent; -, negative.…”

“…In the latest edition of The Yeasts, a Taxonomic Study, six ascosporogenous species (Yamadazyma philogaea, Y. akitaensis, Y. mexicana, Y. nakazawae, Y. scolyti, Y. triangularis) and 23 asexual species assigned to the genus Candida were placed in the Yamadazyma clade . Since then, additional species of the genus Candida in this clade have been found Nakase et al, 2008;AmIn et al, 2011;Buddie et al, 2011;Burgaud et al, 2011;Groenewald et al, 2011) and many novel species of the genus Yamadazyma have been discovered and described such as Yamadazyma terventina, Y. siamensis, Y. phyllophila, Y. paraphyllophila, Y. ubonensis, Y. insecticola, Y. epiphylla, Y. riverae, Y. dushanensis and Y. kitorensis (Ciafardini et al, 2013;Kaewwichian et al, 2013b;Junyapate et al, 2014;Jindamorakot et al, 2015;Lopes et al, 2015;Wang et al, 2015;Nagatsuka et al, 2016). In addition, three known species of the genus Candida were transferred to the genus Yamadazyma as novel combinations, namely Yamadazyma olivae, Y. tumulicola and Y. takamatsuzukensis (Nagatsuka et al, 2016).…”

Yamadazyma cocois f.a., sp. nov., an ascomycetous yeast isolated from coconuts

“…ubonensis [5][6][7][8][9][10][11][12][13]. Although, some ideas about re-evaluation of Yamadazyma clade exist [5,12], but currently there are no solid arguments to validate them.…”

Yamadazyma riverae sp. nov., a yeast species isolated from plant materials

“…Kurtzman & Suzuki (2010) examined phylogenetic relationships among species of the genus Picha that form CoQ-9 and proposed a new circumscription for the genus Yamadazyma , with six ascosporogenous species and 11 asexual species assigned to the genus Candida . Other species of the genus Yamadazyma were described recently as Y. terventina , Y. siamensis , Y. phyllophila , Y. paraphyllophila , Y. ubonensis , Y. insecticola and Y. epiphylla (Ciafardini et al , 2013; Kaewwichian et al , 2013; Junyapate et al , 2014; Jindamorakot et al , 2015). The recently described species Candida khao-thaluensis , Candida oceani , Candida vaughaniae and Candida tallmaniae also belong to the Yamadazyma clade (Nakase et al , 2008; Burgaud et al , 2011; Groenewald et al , 2011).…”