Yap1 homologs mediate more than the redox regulation of the antioxidant response in filamentous fungi

Mendoza-Martínez, Ariann E.; Cano-Domínguez, Nallely; Aguirre, Jesús

doi:10.1016/j.funbio.2019.04.001

Cited by 36 publications

(39 citation statements)

References 101 publications

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“…The RNA-seq data showed that RglT regulates many genes involved in oxidation-reduction processes ( S2 File ), which are important for resistance against oxidative stress that is exerted by the mammalian immune system in order to fight fungal infections [ 25 ]. To determine whether oxidative stress resistance genes are under the transcriptional control of RglT, we assessed the expression of the TF yap1 (Afu6g09930, major factor in the control of the fungal response to oxidative stress [ 26 ]), the mycelial catalase cat1 (Afu3g02270), the superoxide dismutases sod1 (Afu5g0924) and sod2 (Afu4g11580), the cytosolic peroxiredoxin prx1 (Afu4g08580), the mitochondrial peroxiredoxin prxB (Afu5g15070) and the thioredoxin reductase trr1 (Afu4g12990) by qRT-PCR in the WT and Δ rglT strains when grown in the presence of 10 and 30 mM AA for 1 h (short incubation) and 3 h (prolonged incubation) ( S4A Fig ). In these conditions, RglT positively regulates the expression of yap1 , prxB and trr1 ; and negatively regulates the expression of cat1 and sod1 , whereas no induction occurred for sod2 and prx1 ( S4A Fig ).…”

Section: Resultsmentioning

confidence: 99%

“…Yap1 has been described as the major regulator of the A . fumigatus response against oxidative stress [ 26 , 35 ], although it is not required for virulence in a chemotherapeutic murine model of IPA [ 35 ]. This discrepancy may be explained by the fact that RglT regulates many additional genes required for oxidative stress resistance, such as a variety of oxidoreductases that have not been characterized to date.…”

Section: Discussionmentioning

confidence: 99%

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The Aspergillus fumigatus transcription factor RglT is important for gliotoxin biosynthesis and self-protection, and virulence

Ries

Pardeshi

Dong³

et al. 2020

PLoS Pathog

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Aspergillus fumigatus is an opportunistic fungal pathogen that secretes an array of immune-modulatory molecules, including secondary metabolites (SMs), which contribute to enhancing fungal fitness and growth within the mammalian host. Gliotoxin (GT) is a SM that interferes with the function and recruitment of innate immune cells, which are essential for eliminating A . fumigatus during invasive infections. We identified a C6 Zn cluster-type transcription factor (TF), subsequently named RglT, important for A . fumigatus oxidative stress resistance, GT biosynthesis and self-protection. RglT regulates the expression of several gli genes of the GT biosynthetic gene cluster, including the oxidoreductase-encoding gene gliT , by directly binding to their respective promoter regions. Subsequently, RglT was shown to be important for virulence in a chemotherapeutic murine model of invasive pulmonary aspergillosis (IPA). Homologues of RglT and GliT are present in eurotiomycete and sordariomycete fungi, including the non-GT-producing fungus A . nidulans , where a conservation of function was described. Phylogenetically informed model testing led to an evolutionary scenario in which the GliT-based resistance mechanism is ancestral and RglT-mediated regulation of GliT occurred subsequently. In conclusion, this work describes the function of a previously uncharacterised TF in oxidative stress resistance, GT biosynthesis and self-protection in both GT-producing and non-producing Aspergillus species.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Aspergillus fumigatus transcription factor RglT is important for gliotoxin biosynthesis and self-protection, and virulence

Ries

Pardeshi

Dong³

et al. 2020

PLoS Pathog

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“…Our work has been oriented to demonstrate that ROS play critical signaling roles in cell differentiation (Hansberg and Aguirre, 1990;Aguirre et al, 2005;Mendoza-Martinez et al, 2017;Mendoza-Martínez et al, 2019). In one approach, we have used the filamentous fungi Aspergillus nidulans to study the mechanisms by which cells perceive and respond to external ROS.…”

Section: Introductionmentioning

confidence: 99%

DnmA and FisA Mediate Mitochondria and Peroxisome Fission, and Regulate Mitochondrial Function, ROS Production and Development in Aspergillus nidulans

2020

Self Cite

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The dynamin-like protein Drp1 and its receptor Fis-1 are required for mitochondria and peroxisome fission in animal and yeast cells. Here, we show that in the fungus Aspergillus nidulans the lack of Drp1 and Fis-1 homologs DnmA and FisA has strong developmental defects, leading to a notable decrease in hyphal growth and asexual and sexual sporulation, with some of these defects being aggravated or partially remediated by different carbon sources. Although both DnmA and FisA, are essential for mitochondrial fission, participate in peroxisomal division and are fully required for H 2 O 2-induced mitochondrial division, they also appear to play differential functions. Despite their lack of mitochondrial division, dnmA and fisA mutants segregate mitochondria to conidiogenic cells and produce viable conidia that inherit a single mitochondrion. During sexual differentiation, dnmA and fisA mutants develop fruiting bodies (cleistothecia) that differentiate excessive ascogenous tissue and a reduced number of viable ascospores. dnmA and fisA mutants show decreased respiration and notably high levels of mitochondrial reactive oxygen species (ROS), which likely correspond to superoxide. Regardless of this, dnmA mutants can respond to an external H 2 O 2 challenge by re-localizing the MAP kinase-activated protein kinase (MAPKAP) SrkA from the cytoplasm to the nuclei. Our results show that ROS levels regulate mitochondrial dynamics while a lack of mitochondrial fission results in lower respiration, increased mitochondrial ROS and developmental defects, indicating that ROS, mitochondrial division and development are critically interrelated processes.

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“…leading to rapid nuclear import and transcriptional activity (Fernandes et al, 1997;Rodrigues-Pousada et al, 2019). Given that production of ROS forms a major component of plant immune responses, a role for Yap1 during infection has been explored in a number of phytopathogens (Camejo et al, 2016;Mendoza-Martínez et al, 2019;Segal & Wilson, 2018). Consistent with this connection, Yap1 orthologues were shown to be important for virulence in Alternaria alternata, C. gloeosporioides, M. oryzae, and U. maydis (Guo et al, 2011;Li et al, 2017;Lin et al, 2009;Molina & Kahmann, 2007).…”

Section: The Bzip Familymentioning

confidence: 90%

Transcription factor control of virulence in phytopathogenic fungi

John

Singh

Oliver

et al. 2021

Molecular Plant Pathology

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The symptoms of plant diseases such as rusts, blasts, smuts, blotches, blights, and mildews, along with various prescriptions for their mitigation, have been recorded since antiquity (Dark & Gent, 2001;Dugan, 2008;Wu et al., 2019). Until the establishment of the germ theory of disease in the 19th century, the nature of the causal agents remained obscure (Kelman & Peterson, 2002). Subsequently, numerous plant-pathogenic microorganisms have been identified (Crous et al.

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Yap1 homologs mediate more than the redox regulation of the antioxidant response in filamentous fungi

Cited by 36 publications

References 101 publications

The Aspergillus fumigatus transcription factor RglT is important for gliotoxin biosynthesis and self-protection, and virulence

The Aspergillus fumigatus transcription factor RglT is important for gliotoxin biosynthesis and self-protection, and virulence

DnmA and FisA Mediate Mitochondria and Peroxisome Fission, and Regulate Mitochondrial Function, ROS Production and Development in Aspergillus nidulans

Transcription factor control of virulence in phytopathogenic fungi

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