YBX1 promotes tumor growth by elevating glycolysis in human bladder cancer

Xu, Liuyu; Liu, Hongyun; Wu, Longchao; Huang, Shiming

doi:10.18632/oncotarget.19583

Cited by 35 publications

(34 citation statements)

References 26 publications

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“…Hence, long-term expression of YB-1 might terminate the MyoDmediated differentiation of myocytes after forming myotubes in ischemic limbs. Recently, the YB-1 was reported to directly regulate hypoxia-inducible factor (HIF) 1α, a critical transcription factor that regulates adaptive responses to hypoxia and plays a pivotal role in wound healing (El-Naggar et al 2015;Xu et al 2017). Although the association between YB-1 and HIF1α was not investigated in the present study, it is possible that the increased expression of YB-1 after ischemic injury could enhance wound healing via increased expression of HIF1α.…”

Section: Discussionmentioning

confidence: 73%

Increased Expression of Y-Box-Binding Protein-1 in Hind-Limb Muscles During Regeneration from Ischemic Injury in Mice

Fuke

Narita

Wada

et al. 2018

Tohoku J. Exp. Med.

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Critical limb ischemia (CLI) is the most severe complication of peripheral arterial disease (PAD). Understanding the molecular mechanisms underlying tissue repair after CLI is necessary for preventing PAD progression. Y-box binding protein-1 (YB-1) regulates the expression of many genes in response to environmental stresses. We aimed to determine whether YB-1 is involved in ischemic muscle regeneration. A mouse ischemic hind-limb model was generated; namely, the femoral, saphenous, and popliteal arteries in the left hind limb were ligated. The right hind limb, with skin incisions alone, served as control. Hind limbs (n = 3-5 for each time point) were examined on day 0 (before the operation) and on postoperative days 1, 2, 7, 10, and 14, and the biceps femoris, adductor, rectus femoris, and gracilis muscles were subjected to histopathological and immunohistochemical analyses. In ischemic limbs, myogenesis, triggered by an increase in myotubes, began on day 7; thereafter, regenerated muscles gradually increased in volume. RT-PCR analysis showed that YB-1 mRNA levels were increased in the limbs after ischemic injury, peaked on day 2, and subsequently decreased. On day 7, expression levels of MyoD and alphasmooth muscle actin (αSMA) mRNAs were significantly higher in ischemic muscles than in control muscles. Immunohistochemical analysis revealed increased YB-1 immunoreactivity in myoblasts and myotubes on day 7, which was decreased by day 14. The immunoreactive αSMA and smooth muscle myosin heavy chain were transiently increased in myotubes. This is the first report showing the increased expression of YB-1 during muscle regeneration after ischemic injury.

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Section: Discussionmentioning

confidence: 73%

Increased Expression of Y-Box-Binding Protein-1 in Hind-Limb Muscles During Regeneration from Ischemic Injury in Mice

Fuke

Narita

Wada

et al. 2018

Tohoku J. Exp. Med.

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“…For example, YB1 was involved in cell migration, invasion, and promoted epithelial-to-mesenchymal transition (EMT) in human breast cancer cell lines 11 . Furthermore, in human bladder cancer, YB1 was able to regulate the expression of c-Myc and HIF1α to enhance glycolysis, and therefore promoted tumor growth and inhibit apoptosis 24 . YB1 promoted lung cancer stem-like properties and metastasis both in vitro and in vivo 25 .…”

Section: Discussionmentioning

confidence: 99%

The Role of YB1 in Renal Cell Carcinoma Cell Adhesion

Wang¹,

Su²,

Fu³

et al. 2018

Int. J. Med. Sci.

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Background: Y-box binding protein 1 (YB1) is a multifunctional protein involved in many processes related to cancer progression and metastasis.Methods: In this study, we constructed YB1 knockdown stable renal cell carcinoma (RCC) cell line 786-0. The gene expression profile of 786-0 was performed by DNA microarray analysis to identify genes that were regulated by YB1. Real-time PCR and western blotting were used to test the genes and proteins expression. Transforming growth factor-β (TGF-β) activity was detected by dual-luciferase reporter assay. Cell adhesion assay was used to determine RCC cell adhesion ability.Results: Pathway analysis revealed that YB1 knockdown influenced cell adhesion molecules (CAMs). We further verified four genes (CLDN4, NRXN3, ITGB8, and VCAN) related to CAMs by real-time PCR, and confirmed that YB1 regulated the expression of ITGB8 in RCC. Functional assays demonstrated that knockdown of YB1 significantly inhibited the cell adhesion of 786-0 cells in vitro. In addition, YB1 affected TGF-β activation.Conclusion: Our study demonstrated that YB1 modulated the adhesion ability of renal cell carcinoma cells by regulating ITGB8 and TGF-β.

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“…Incidentally, FOXM1 is a classic regulator of aerobic glycolysis (“Warburg effect”) (Cui et al, 2014; Shang et al, 2017; Wang et al, 2016; Zheng et al, 2016). MLX and YBX-1 are known glycolytic shift regulators—controlling retrograde mitochondrial signaling to the nucleus, expression of glycolytic and lipogenic genes, and integrating PI3K/AKT signaling to glycolysis (Amal et al, 2015; Billin and Ayer, 2006; Butow and Avadhani, 2004; Diolaiti et al, 2015; Lasham et al, 2013; Sans et al, 2006; Stoltzman et al, 2008; Suresh et al, 2018; Xu et al, 2017). MED27, MRPL12 and ELP5 further contribute to these activities (Alexander et al, 2013; Catarina et al, 2014; Close et al, 2012; Fisher, 2018; Frei et al, 2005; Hawer et al, 2018; Rapino and Close, 2018).…”

Section: Resultsmentioning

confidence: 99%

Single-cell based elucidation of molecularly-distinct glioblastoma states and drug sensitivity

Ding

Burgenske

Zhao

et al. 2019

Preprint

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Glioblastoma heterogeneity and plasticity remain controversial, with proposed subtypes representing the average of highly heterogeneous admixtures of independent transcriptional states. Single-cell, protein-activity-based analysis allowed full quantification of >6,000 regulatory and signaling proteins, thus providing a previously unattainable single-cell characterization level. This helped identify four novel, molecularly distinct subtypes that successfully harmonize across multiple GBM datasets, including previously published bulk and single-cell profiles and single cell profiles from seven orthotopic PDX models, representative of prior subtype diversity. GBM is thus characterized by the plastic coexistence of single cells in two mutually-exclusive developmental lineages, with additional stratification provided by their proliferative potential. Consistently, all previous subtypes could be recapitulated by single-cell mixtures drawn from newly identified states. Critically, drug sensitivity was predicted and validated as highly state-dependent, both in single-cell assays from patient-derived explants and in PDX models, suggesting that successful treatment requires combinations of multiple drugs targeting these distinct tumor states. Significance We propose a new, 4-subtype GBM classification, which harmonizes across bulk and single-cell datasets. Single-cell mixtures from these subtypes effectively recapitulate all prior classifications, suggesting that the latter are a byproduct of GBM heterogeneity. Finally, we predict single-cell level activity of three clinically-relevant drugs, and validate them in patient-derived explant.

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YBX1 promotes tumor growth by elevating glycolysis in human bladder cancer

Cited by 35 publications

References 26 publications

Increased Expression of Y-Box-Binding Protein-1 in Hind-Limb Muscles During Regeneration from Ischemic Injury in Mice

Increased Expression of Y-Box-Binding Protein-1 in Hind-Limb Muscles During Regeneration from Ischemic Injury in Mice

The Role of YB1 in Renal Cell Carcinoma Cell Adhesion

Single-cell based elucidation of molecularly-distinct glioblastoma states and drug sensitivity

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