Yearly, pond, lineage and family variation of hepatopancreatic parvo-like virus (HPV) copy number in banana shrimp Fenneropenaeus merguiensis

Knibb, Wayne; Quinn, Jane; Kuballa, Anna; Powell, Daniel; Remilton, Courtney; Nguyen, Nguyen Hong

doi:10.1016/j.jip.2015.05.002

Cited by 13 publications

(21 citation statements)

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“…Quantitative polymerase chain reaction (qPCR) reactions were performed with a Rotor-Gene 6000 thermal cycler (Corbett Research). Details about standard curve, components of the qPCR reaction mix and cycling conditions are in [26]. Briefly, a standard curve was generated based on tenfold serially-diluted known concentrations of purified HPV PCR products.…”

Section: Methodsmentioning

confidence: 99%

“…The first group consisted of 897 samples for which HPV titres had not been quantified. The second group included 240 samples for which HPV titres had been determined in our previous study [26]. In this study, HPV titres of 135 samples in the second group were quantified for a second time, whereas the remaining 105 samples (that represented five qPCR batches from our previous work) were simply used in the statistical analyses.…”

Section: Methodsmentioning

confidence: 99%

“…In each batch, samples from at least 23 different families were randomly selected. In total, there were 25 qPCR batches represented in the subsequent analyses, 20 batches from the current study and five batches from the previous study [26]. …”

Section: Methodsmentioning

confidence: 99%

“…Overall, in view of all these potentially desirable aspects of using viral titre as a selection criterion, and notwithstanding the efficacy of viral titre versus survival as a selection criterion, it is remarkable that, until this report and that of Knibb et al [26], there have been no attempts to estimate the heritability of viral titre in an aquaculture species. Here, we address this knowledge gap by using a large dataset and viral copy number as a quantitative measurement to detect putative genetic resistance to HPV and to estimate the heritability of viral titre and its genetic correlations with body, carcass and flesh quality traits in banana shrimp.…”

Section: Introductionmentioning

confidence: 99%

“…Second, viral titre may reflect the innate ability to resist to viruses rather than an ability to survive. There are also a number of other appealing aspects of using viral titre as a measure for viral resistance which have been discussed in our previous study [26]. For example, it does not require a challenge facility to obtain viral titre data; the data can be obtained directly from commercial operations, which makes it highly relevant and applicable to commercial conditions.…”

Section: Introductionmentioning

confidence: 99%

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Moderate heritability of hepatopancreatic parvovirus titre suggests a new option for selection against viral diseases in banana shrimp (Fenneropenaeus merguiensis) and other aquaculture species

et al. 2016

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BackgroundIn shrimp farming, major production losses are caused by viruses. Hepatopancreatic parvovirus (HPV) is one of the viral pathogens that infect banana shrimp (Fenneropenaeus merguiensis). HPV is thought to slow down growth and cause mortality in the juvenile stages of banana shrimp. Genetic improvement through selection of shrimp resistant to viral diseases is one approach to address this issue. This is the first detailed report on an aquaculture species that investigates whether viral titre varies among families and is heritable, and thus whether viral titre per se is a possible candidate trait for selection to produce resistant stock.ResultsHPV titre was measured by quantitative polymerase chain reaction of DNA extracted from 1137 offspring (from 48 full-sib families). Estimated heritability of HPV titre, based on the linear animal mixed model, was moderate (h2 = 0.41). Genetic correlations of HPV with body traits (weight, length and width of body, head and tail) ranged from −0.13 to −0.38. HPV titre was negatively correlated with raw and cooked body colour (−0.33 and −0.43, respectively).ConclusionsThis is the first study based on a large dataset that provides evidence that viral titre may have a genetic component in penaeid shrimp or even in any aquaculture species. The moderate heritability estimated for this trait suggests that resistance to HPV may be achieved by selecting for low HPV titre. With moderate and negative correlations, selection for resistance to HPV should gradually improve body traits and colour of banana shrimp.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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