Yeast (ADPribosyl)ation: Revisiting a controversial question

Faraone-Mennella, Maria Rosaria; Maio, Anna De; Petrella, Anna; Syntichaki, Evangelia; Kerbalaeva, A. M.; Nasmetova, S. M.; Goulyamova, Toshkon G.; Farina, Biancamaria

doi:10.1002/jcb.20362

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…PARP activity was assayed in duplicate in two cell preparations [27]. The reaction mixture (final volume 50 µL)contained 0.5 M Tris-HCl pH 8.0, 50 mM MgCl 2 , 10 mM DTT, 0.4 mM [ 32 P]NAD + (10,000 cpm/nmole) and a defined amount (20 µg protein)of whole lysates.…”

Section: Methodsmentioning

confidence: 99%

Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides

Imperato

Mistretta

Marone

et al. 2019

Cells

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Increased DNA damage and the propension to cancer development, depend on the modulation of the mechanisms to control and maintain genomic integrity. Poly(ADP-Ribose)Polymerase activation and automodification are early responses to genotoxic stress. Upon binding to DNA strand breaks, the enzyme, a molecular DNA nick sensor, is hyperactivated: this is the first step in a series of events leading to either DNA repair or apoptosis. Enzyme hyperactivation and automodification can be easily measured and are widely used to look at DNA damage extent in the cell. We investigated whether these two markers (increased catalytic activity and auto modification), could help to monitor DNA damage in lymphocytes of flower growers from Southern Italy, occupationally exposed to pesticides. Peripheral lymphocyte lysates were analyzed for Poly(ADP-Ribose)Polymerase activity, and by SDS-PAGE and anti-Poly(ADP-Ribose)Polymerase 1-antibodyto measure automodified Poly(ADP-Ribose)Polymerase levels bydensitometry. Poly(ADP-Ribose)Polymerase activity and PARP automodification followed the same trend. Growers daily exposed to pesticides, showed both biomarkers very high, either in the presence or in the absence of pathologies. PARP activity and auto-modification in peripheral blood lymphocytes are possible, non-invasive, androutinartools to monitor the healthy conditions of floricoltorists.

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Section: Methodsmentioning

confidence: 99%

Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides

Imperato

Mistretta

Marone

et al. 2019

Cells

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“…after extensive DNA damage) causes depletion of NAD? and subsequent profound drop of ATP [114] (n.h.) Enzymes with (ADPribosyl)ation activity may exist in yeast [115] PARP-2 Required for zVAD.fmk and TNFa-induced necrosis.…”

Section: Necrotic Cell Death In Yeast-secondary or Primary?mentioning

confidence: 99%

“…May have similar function as PARP-1 [73] (n.h.) Enzymes with (ADPribosyl)ation activity may exist in yeast [115] PKA Phosphorylates glyoxalase-1, which is required for TNFa induced necrosis in L929 cells [116] Tpk1p, Tpk2p, Tpk3p Isoforms of c-AMP-dependent protein kinase (PKA), the effector kinase of the Ras-cAMP signaling pathway Pro-aging signaling pathway [102,117] Glyoxalase-1 Promotes AGE formation [116] Glo1p…”

Section: Necrotic Cell Death In Yeast-secondary or Primary?mentioning

confidence: 99%

Necrosis in yeast

et al. 2010

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Necrosis was long regarded as an accidental cell death process resulting from overwhelming cellular injury such as chemical or physical disruption of the plasma membrane. Such a definition, however, proved to be inapplicable to many necrotic scenarios. The discovery that genetic manipulation of several proteins either protected or enhanced necrotic cell death argued in favor of a regulated and hence programmed process, as it is the case for apoptosis. For more than a decade, yeast has served as a model for apoptosis research; recently, evidence accumulated that it also harbors a necrotic program. Here, we summarize the current knowledge about factors that control necrotic cell death in yeast. Mitochondria, aging and a low pH are positive regulators of this process while cellular polyamines (e.g. spermidine) and endonuclease G as well as homeostatic organelles like the vacuole or peroxisomes are potent inhibitors of necrosis. Physiological necrosis may stimulate intercellular signaling via the release of necrotic factors that promote viability of healthy cells and, thus, assure survival of the clone. Together, the data obtained in yeast argue for the existence of a necrotic program, which controls longevity and whose physiological function may thus be aging.

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Enhancement of the NAD(P)(H) Pool in Saccharomyces cerevisiae

Knepper

Schleicher

Klauke

et al. 2008

Engineering in Life Sciences

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Asymmetric biosyntheses allow for an efficient production of chiral building blocks. The application of whole cells as biocatalysts for asymmetric syntheses is advantageous because they already contain the essential coenzymes NAD(H) or NADP(H), which additionally can be regenerated in the cells. Unfortunately, reduced catalytic activity compared to the oxidoreductase activity is observed in many cases during whole‐cell biotransformation. This may be caused by low intracellular coenzyme pool sizes and/or a decline in intracellular coenzyme concentrations. To enhance the intracellular coenzyme pool sizes, the effects of the precursor metabolites adenine and nicotinic acid on the intracellular accumulation of NAD(H) and NADP(H) were studied in Saccharomyces cerevisiae. Based on the results of simple batch experiments with different precursor additions, fed‐batch processes for the production of yeast cells with enhanced NAD(H) or enhanced NADP(H) pool sizes were developed. Supplementation of the feed medium with 95 mM adenine and 9.5 mM nicotinic acid resulted in an increase of the intracellular NAD(H) concentration by a factor of 10 at the end of the fed‐batch process compared to the reference process. The final NAD(H) concentration remains unchanged if the feed medium was solely supplemented with 95 mM adenine, but intracellular NADP(H) was increased by a factor of 4. The effects of NADP(H) pool sizes on the asymmetric reduction of ethyl‐4‐chloro acetoacetate (CAAE) to the corresponding (S)‐4‐chloro‐3‐hydroxybutanoate (S‐CHBE) was evaluated with S. cerevisiae FasB His6 as an example. An intracellular threshold concentration above 0.07 mM NADP(H) was sufficient to increase the biocatalytic S‐CHBE productivity by 25 % compared to lower intracellular NADP(H) concentrations.

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Yeast (ADPribosyl)ation: Revisiting a controversial question

Cited by 6 publications

References 41 publications

Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides

Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides

Necrosis in yeast

Enhancement of the NAD(P)(H) Pool in Saccharomyces cerevisiae

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