Yeast-based production and in situ purification of acetaldehyde

Abstract

Acetaldehyde is a platform chemical with a production volume of more than 1 Mt/a, but is chiefly synthesized from petrochemical feedstocks. We propose the fermentative conversion of glucose towards acetaldehyde via genetically modified S. cerevisiae. This allows for ethanol-free bioactaldehyde production. Exploiting the high volatility of the product, in situ gas stripping in an aerated reactor is inevitable and crucial due to the respiratory toxicity effects of the acetaldehyde overproduction. We devise a lab-scale setup for the recovery of the product from the off-gas. Water was chosen as a suitable solvent and the Henry coefficient of acetaldehyde in water was validated experimentally. Based on an experimentally verified capture efficiency of 75%, an acetaldehyde production rate of over 100 mg/g/h was reached in 200 mL lab-scale fermentations.

Biocatalytic Furfuryl Alcohol Production with Ethanol as the Terminal Reductant Using a Single Enzyme

“…Total product selectivity and atom economy are distinct advantages of cell-free enzymatic processes vs whole-cell biotransformation . Previous works with EtOH-dependent cell-free enzymatic biotransformation demonstrated that several NAD­(P)­H-dependent redox enzymes, including YADH, are kinetically capable of converting FAL to FOL. , Acetaldehyde, the co-product of EtOH-dependent FAL reduction, is an essential platform chemical characterized by high vapor pressure at room temperature, which can facilitate its in situ separation . YADH has a high turnover frequency for EtOH oxidation and broad substrate specificity .…”

“…36,37 Acetaldehyde, the co-product of EtOHdependent FAL reduction, is an essential platform chemical characterized by high vapor pressure at room temperature, which can facilitate its in situ separation. 38 YADH has a high turnover frequency for EtOH oxidation 39 and broad substrate specificity. 40 Li et al showed that furfural reductase (FurX) from Cupriavidus necator JMP134 had higher catalytic efficiency than YADH for EtOH-dependent FAL reduction (k cat , 4-fold higher than YADH).…”

Three Sides of the Same Coin: Combining Microbial, Enzymatic, and Organometallic Catalysis for Integrated Conversion of Renewable Carbon Sources

“…Mengers et al. 63 proposed a system for the yeast‐based production of acetaldehyde with in situ gas stripping and capture (Fig. 3).…”

“…This can be avoided by combining whole-cell and enzyme catalysis. Mengers et al [63] proposed a system for the yeast-based production of acetaldehyde with in situ gas stripping and capture (Fig. 3).…”