Yeast copper-dependent transcription factor ACE1 enhanced copper stress tolerance in Arabidopsis

Abstract

Copper is essential but toxic in excess for aerobic organisms. Yeast transcription factor ACE1 functions as a sensor for copper and an inducer for the transcription of CUP1. In addition, ACE1 can activate the transcription of superoxide dismutase gene (sod1) in response to copper. In this study, we introduced the yeast ACE1 into Arabidopsis and analyzed its function in plant. Under high copper stress, the transgenic plants over-expressing ACE1 showed higher survival rate than the wild-type. We also found that over-expression of ACE1 in Arabidopsis increased the activities of SOD and POD, which were beneficial to the cell in copper buffering. Excess copper would suppress the expression of chlorophyll biosynthetic genes in Arabidopsis, RT-PCR analysis revealed that over-expression of

MAC Family Transcription Factors Enhance the Tolerance of Mycelia to Heat Stress and Promote the Primordial Formation Rate of Pleurotus ostreatus

“…In addition, the function of MAC1 has been extensively studied in other species; for example, in yeast, the functional defect mutation of MAC1 can lead to an increase in yeast cell thermal sensitivity [20] In Arabidopsis, there is a copper sensing transcription factor ACE1, which has a highly similar structure to MAC1. Moreover, OE-ACE1 transgenic plants have a higher survival rate under copper stress than WT plants, while increasing the activity of SOD and POD [16]. The results of this study are similar to those of previous studies, indicating that MAC1 can participate in various abiotic stress response pathways.…”

“…Overexpression of the methionine sulfoxide reductase gene enhances the stress tolerance of P. ostreatus [14]. Many genes and transcription factors have also been proven to play an important role in the emergence stage of mushrooms [15,16]. For example, the PDD1 transcription factor positively regulates the emergence of Flammulina filiformis and can increase their yield [17].…”

Metallothioneins of the urochordateOikopleura dioicahave Cys-rich tandem repeats, large size and cadmium-binding preference

“…65 Although CUP2 orthologous have not been identified outside fungal species, ACE1/AMT1 sequences have been related to transcriptional regulation of Cu/ZnSOD1 genes in maize 66 and Arabidopsis. 67 Therefore, we also analyzed the promoters (1 kb upstream sequence from the CDS) of the five SOD1 genes predicted in O. dioica genome, and interestingly, we identified MREF (ACE1/AMT1) sequences in four of them ( Fig. 2A), suggesting that as described in fungi, MT and SOD genes shared a common transcriptional response in O. dioica.…”

Comparison of global responses to mild deficiency and excess copper levels in Arabidopsis seedlings

“…However, most gene expression analyses under Cu excess conditions have been carried out at high Cu concentrations (25-50 mM), where reduced growth and other physiological symptoms are already evident. [25][26][27][28][29] The main objectives of the current work are to define Cu sufficiency limits at the molecular level and to provide new molecular markers for mild Cu deficiency and excess conditions. For these purposes, global changes in the gene expression under mild Cu deficiency and Cu excess conditions were compared.…”