Yeast-Derived ? -Glucan Reduces Intestinal Injury in Rat Model of Necrotizing Enterocolitis

Větvička, Václav; Arriola, Jennifer; Kaveti, Ashwini; Dvořák, Bohuslav

doi:10.15406/icpjl.2015.01.00017

Cited by 6 publications

(6 citation statements)

References 31 publications

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“…In addition, we found β-glucan also effectively inhibited the expression of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β, which is in accordance with the results reported by Minmin Hul [49]. A previous study has also [33]. Moreover, the results of molecular docking also showed that β-glucan had a high affinity for the TLR4 binding site.…”

Section: Discussionsupporting

confidence: 91%

“…In addition, we found β-glucan also effectively inhibited the expression of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β, which is in accordance with the results reported by Minmin Hul [ 49 ]. A previous study has also demonstrated that yeast-derived β-glucan could reduce intestinal injury in rat model of NEC by affecting intestinal expression of IL-18, TNF-α, iNOS, CXCL1, Tff3, and Muc-2, which suggests that β-glucan might possess potential in the treatment of NEC via reduction of inflammatory response within the intestine [ 33 ]. Moreover, the results of molecular docking also showed that β-glucan had a high affinity for the TLR4 binding site.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that fungal β-glucan inhibited the release of lipopolysaccharide (LPS)-induced nitric oxide and tumor necrosis factor (TNF)-α in vitro and reduced the secretion of TNF-α and interleukin (IL)-6 in vivo [ 31 , 32 ]. In a previous study, for the first time, Vetvicka et al reported that supplementation of β-glucan reduced the incidence and severity of NEC in a neonatal rat model via reduction of inflammatory response within the intestine [ 33 ]. However, the precise mechanism by which β-glucans inhibit the release of inflammatory cytokines and induce anti-inflammatory immune cells is complex and not fully understood.…”

Section: Introductionmentioning

confidence: 99%

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β-glucan protects against necrotizing enterocolitis in mice by inhibiting intestinal inflammation, improving the gut barrier, and modulating gut microbiota

Zhang

et al. 2023

J Transl Med

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Background Necrotizing enterocolitis (NEC) is a devastating gastrointestinal disease with high morbidity and mortality, affecting preterm infants especially those with very low and extremely low birth weight. β-glucan has manifested multiple biological effects including anti-inflammatory, regulation of gut microbiota, and immunomodulatory activities. This study aimed to investigate the effects of β-glucan on NEC. Methods Neonatal C57BL/6 mice were randomly divided into three groups: Control group, NEC group and β-glucan group. Newborn 3-day-old mice were gavaged with either 1 mg/ml β-glucan or phosphate buffer saline at 0.03 ml/g for 7 consecutive days before NEC induction and a NEC model was established with hypoxia combined with cold exposure and formula feeding. All the pups were killed after 72-h modeling. Hematoxylin–eosin staining was performed to assess the pathological injury to the intestines. The mRNA expression levels of inflammatory factors in intestinal tissues were determined using quantitative real-time PCR. The protein levels of TLR4, NF-κB and tight junction proteins in intestinal tissues were evaluated using western blotting and immunohistochemistry. 16S rRNA sequencing was performed to determine the structure of the gut microbiota. Results β-glucan administration ameliorated intestinal injury of NEC mice; reduced the intestinal expression of TLR4, NF-κB, IL-1β, IL-6, and TNF-α; increased the intestinal expression of IL-10; and improved the expression of ZO-1, Occludin and Claudin-1 within the intestinal barrier. Pre-treatment with β-glucan also increased the proportion of Actinobacteria, Clostridium butyricum, Lactobacillus johnsonii, Lactobacillus murinus, and Lachnospiraceae bacterium mt14 and reduced the proportion of Klebsiella oxytoca g Klebsiella in the NEC model. Conclusion β-glucan intervention prevents against NEC in neonatal mice, possibly by suppressing the TLR4-NF-κB signaling pathway, improving intestinal barrier function, and partially regulating intestinal microbiota.

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“…In addition, we found β-glucan also effectively inhibited the expression of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β, which is in accordance with the results reported by Minmin Hul [49]. A previous study has also [33]. Moreover, the results of molecular docking also showed that β-glucan had a high affinity for the TLR4 binding site.…”

Section: Discussionsupporting

confidence: 91%

“…In addition, we found β-glucan also effectively inhibited the expression of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β, which is in accordance with the results reported by Minmin Hul [ 49 ]. A previous study has also demonstrated that yeast-derived β-glucan could reduce intestinal injury in rat model of NEC by affecting intestinal expression of IL-18, TNF-α, iNOS, CXCL1, Tff3, and Muc-2, which suggests that β-glucan might possess potential in the treatment of NEC via reduction of inflammatory response within the intestine [ 33 ]. Moreover, the results of molecular docking also showed that β-glucan had a high affinity for the TLR4 binding site.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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β-glucan protects against necrotizing enterocolitis in mice by inhibiting intestinal inflammation, improving the gut barrier, and modulating gut microbiota

Zhang

et al. 2023

J Transl Med

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“…With over 15,000 published papers, it is not surprising that more and more attention is focused onβ-glucan in medicine. Effects of β-glucan supplementation on cancer growth, allergy, autoimmune diseases, gastrointestinal tract problems, infections and other pathophysiological problems were repeatedly demonstrated [13][14][15][16] (for review see Vetvicka [17] , Vannucci [18] . Our own experience with the positive effects of the addition of glucan to the diet of patients finishing their cancer treatment on cellular immunity led us to this study evaluating effects of glucan supplementation on humoral immunity and on possible elimination of stress and additional side effects of cancer treatment.…”

Section: Discussionmentioning

confidence: 99%

Effect of β-glucan Supplementation on Levels of IgM, IgA, IgG and Its Subclasses IgG1, IgG2, IgG3, and IgG4 in Cancer Patients

et al. 2016

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AIM:The role of β-glucan supplementation on cancer development is well established. In this report we focused on the effects of glucan on levels of individual classes and subclasses of immunoglobulins. MATERIALS AND METHODS:We evaluated the levels of IgM, IgG, IgA, IgG1, IgG2, IgG3 and IgG4 in 39 patients diagnosed with breast or colorectal cancer. RESULTS: We found a significant decrease of IgG1 levels in the placebo group, which was particularly pronounced in females. CONCLUSIONS: Short-term glucan supplementation significantly stabilized levels of IgG1 resulting in maintaining anti-infectious immunity. The decrease in IgG1 levels found in the placebo group was clearly caused by a complex therapy of cancer disease.

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“…Glucans were also found to be active in the reduction of intestinal injury (44), in protection against liver injury (45), in regulation of hematopoiesis of patients with cancer (46), and in the improvement of benign hyperplasia of the prostate (9). Because glucans have been shown to stimulate the immune system directly, they can be useful in alleviating the symptoms of inflammatory conditions such as irritable bowel syndrome (47).…”

Section: Additional Effectsmentioning

confidence: 99%

Glucans as New Anticancer Agents

2019

Self Cite

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For decades, glucans have been studied for their biological and immunological activities. The scientific community has mainly focused on immune reactions, but other areas, such as cholesterol levels and diabetic retinopathy, have shown the positive effects of glucan supplementation. However, the majority of studies focused on possible cancer suppression, where glucans showed clear and significant effects on numerous types of cancers, leading not only to clinical trials, but to approval as an official drug. The aim of this review is to describe the current knowledge of this field.

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Yeast-Derived ? -Glucan Reduces Intestinal Injury in Rat Model of Necrotizing Enterocolitis

Cited by 6 publications

References 31 publications

β-glucan protects against necrotizing enterocolitis in mice by inhibiting intestinal inflammation, improving the gut barrier, and modulating gut microbiota

β-glucan protects against necrotizing enterocolitis in mice by inhibiting intestinal inflammation, improving the gut barrier, and modulating gut microbiota

Effect of β-glucan Supplementation on Levels of IgM, IgA, IgG and Its Subclasses IgG1, IgG2, IgG3, and IgG4 in Cancer Patients

Glucans as New Anticancer Agents

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