Yeast-derived potato patatins: Biochemical and biophysical characterization

Gelley, Sivan; Lankry, Hodaya; Glušac, Jovana; Fishman, Ayelet

doi:10.1016/j.foodchem.2021.130984

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…However, the size distribution curve in particle number frequency of the untreated Po‐PI dispersion was monomodal with a peak maximum at 8 nm and a mean diameter of 8.1 ± 0.6 nm. In fact, ~94% of the protein particles of the non‐processed dispersion were smaller than 10 nm in diameter in accordance with previous results on the same commercial patatin‐rich isolate (Baier & Knorr, 2015; Andlinger et al ., 2022; Gelley et al ., 2022). As the pressurisation time increased, the size distribution curve in intensity was modified (Fig.…”

Section: Resultsmentioning

confidence: 99%

Long‐time high‐pressure processing of a patatin‐rich potato proteins isolate: impact on aggregation and surface properties

Bahri,

Charpentier,

Khati

et al. 2024

Int J of Food Sci Tech

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SummaryIn this study, a 4% (w/w) dispersion of a commercial patatin‐rich potato protein isolate (Po‐PI) was pressurised at 400 MPa up to 48 h at 20 °C. Protein aggregation induced by high‐pressure processing (HHP) was followed by dynamic light scattering, intrinsic fluorescence (in‐situ or ex‐situ) or SAXS analysis. Surface properties (surface hydrophobicity and interfacial properties) of the HHP‐induced aggregates were also investigated. A gradual dimer dissociation/protein unfolding was observed under pressure. Po‐PI exhibited a slow relaxation time under pressure. Long‐time HHP (>4 h) induced significant modification of the Po‐PI protein structure with partial non‐reversible unfolding. After 48 h of pressurisation at 400 MPa, large aggregates (160 nm) were obtained and a monomodal distribution in intensity and in number frequency was observed indicating a controlled aggregation. Up to 24 h of pressurisation at 400 MPa, intermediate states were obtained after high‐pressure release. SDS‐PAGE profiles showed that HHP‐induced aggregation of Po‐PI was driven by non‐covalent interactions. All high‐pressure processed dispersions displayed a higher surface hydrophobicity as compared to non‐treated Po‐PI. Po‐PI dispersion treated for 8 h at 400 MPa presented the lowest adsorption rate, the highest final surface tension and formed the most rigid interfacial film. Po‐PI showed resistance to moderate pressure levels (400 MPa) and long pressure application times were required to induce significant protein denaturation/aggregation (≥24 h) and to optimally modify its interfacial properties (8 h).

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Section: Resultsmentioning

confidence: 99%

Long‐time high‐pressure processing of a patatin‐rich potato proteins isolate: impact on aggregation and surface properties

Bahri,

Charpentier,

Khati

et al. 2024

Int J of Food Sci Tech

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“…Specifically, cupuassu has a positively selected and tandemly duplicated gene related to lipid storage in fruits, known as patatin (TgrandC1074G00000017909 and TgrandC1074G00000017911). Originally identified in potato ( Solanum tuberosum L. ) tubers, patatin is renowned for its antioxidant potential [98] and its exceptional nutritional value, making it an appealing food additive due to its solubility and emulsifying properties [99,100].…”

Section: Resultsmentioning

confidence: 99%

“…In contrast, T. cacao features a tandem duplicated phospholipase A1 positively selected (Tcacao-CriolloG00000024071 and Tcacao-CriolloG00000024072), which could modulate the fruit phospholipid profile. For instance, this phospholipase may be involved in linoleic acid metabolism [99][100][101], central to the production of desaturated fatty acids present in cacao-derived chocolates [78]. Meanwhile, T. grandiflorum, displays a tandem repeated gene encoding a fatty acyl-CoA reductase enzyme (TgrandC1074G00000003252 and TgrandC1074G00000003253), potentially influencing the lipid content and composition of seeds, impacting wax biosynthesis [102] and, by extension, the fruit cuticle, water retention, and shelf life.…”

Section: Positively Selected Retained Dispersed Proximal and Tandem D...mentioning

confidence: 99%

Genomic decoding ofTheobroma grandiflorum(cupuassu) at chromosomal scale: Evolutionary insights for horticultural innovation

Alves,

de Abreu,

Oliveira

et al. 2023

Preprint

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BackgroundTheobroma grandiflorum(Malvaceae), known as cupuassu, is a tree indigenous to the Amazon Basin, valued for its large fruits and seed-pulp, contributing notably to the Amazonian bioeconomy. The seed-pulp is utilized in desserts and beverages, and its seed butter is used in cosmetics. Here, we present the sequenced telomere-to-telomere cupuassu genome, disclosing features of the genomic structure, evolution, and phylogenetic relationships within the Malvaceae.ResultsThe cupuassu genome spans 423 Mb, encodes 31,381 genes distributed in the ten chromosomes, and it exhibits approximately 65% gene synteny with theT. cacaogenome, reflecting a conserved evolutionary history, albeit punctuated with unique genomic variations. The main changes are pronounced by bursts of long-terminal repeats retrotransposons expansion at post-species divergence, retrocopied and singleton genes, and gene families displaying distinctive patterns of expansion and contraction. Furthermore, positively selected genes are evident, particularly among retained and dispersed, tandem and proximal duplicated genes associated to general fruit and seed traits and defense mechanisms, supporting the hypothesis of potential episodes of subfunctionalization and neofunctionalization following duplication, and impact from distinct domestication process. These genomic variations may underpin the differences observed in fruit and seed morphology, ripening, and disease resistance between cupuassu and the other Malvaceae species.ConclusionsSequencing the cupuassu genome offers a foundational resource for both breeding and conservation efforts, yielding insights into the evolution and diversity within the genusTheobroma.Core ideasTelomere-to-telomere sequencing of theTheobroma grandiflorumgenome elucidates a 65% synteny withT. cacao.Retrotransposon expansion identified as a pivotal factor in post-divergence genomic evolution betweenTheobromaspecies.Comparative genomics has revealed genes associated with key agronomic traits, providing evolutionary insights.Positive selection pressure in retained duplicated genes implicated in adaptive functions and fruit-seed traits diversity.Cupuassu genome as a genetic resource for breeding and to boosts Brazillian Amazonian bioeconomy.AUTHOR SUMMARYCupuassu, a fruit-bearing tree from the Amazon, is prized for its nutritious fruits and seeds, widely used in food and cosmetics. In this study, we sequenced the complete genome of cupuassu to understand its development, unique traits, and genetic relationship with cacao and other related species. The cupuassu genome shares a high similarity with cacao, but it also exhibits distinctive features. Notably, repetitive DNA elements have significantly influenced its genomic structure. Furthermore, specific genes responsible for its fruit and seed characteristics, as well as disease resistance, were identified. Overall, this research not only deepens our knowledge of cupuassu genetics but also illuminates broader aspects of plant evolution and diversity in the Amazon. It lays the groundwork for advanced breeding programs and promises to contribute significantly to the Amazonian bioeconomy. Ultimately, these findings have important implications for agriculture and conservation, highlighting the intricate processes of plant adaptation and evolution.

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“…Specifically, cupuassu has a positively selected and tandemly duplicated gene related to lipid storage in fruits, known as patatin (TgrandC1074G00000017909 and TgrandC1074G00000017911). Originally identified in potato ( Solanum tuberosum L.) tubers, patatin is renowned for its antioxidant potential [ 124 ] and its exceptional nutritional value, making it an appealing food additive due to its solubility and emulsifying properties [ 125 , 126 ].…”

Section: Methodsmentioning

confidence: 99%

Genomic decoding of Theobroma grandiflorum (cupuassu) at chromosomal scale: evolutionary insights for horticultural innovation

Alves,

de Abreu,

Oliveira

et al. 2024

GigaScience

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Background Theobroma grandiflorum (Malvaceae), known as cupuassu, is a tree indigenous to the Amazon basin, valued for its large fruits and seed pulp, contributing notably to the Amazonian bioeconomy. The seed pulp is utilized in desserts and beverages, and its seed butter is used in cosmetics. Here, we present the sequenced telomere-to-telomere genome of cupuassu, disclosing its genomic structure, evolutionary features, and phylogenetic relationships within the Malvaceae family. Findings The cupuassu genome spans 423 Mb, encodes 31,381 genes distributed in 10 chromosomes, and exhibits approximately 65% gene synteny with the Theobroma cacao genome, reflecting a conserved evolutionary history, albeit punctuated with unique genomic variations. The main changes are pronounced by bursts of long-terminal repeat retrotransposons at postspecies divergence, retrocopied and singleton genes, and gene families displaying distinctive patterns of expansion and contraction. Furthermore, positively selected genes are evident, particularly among retained and dispersed tandem and proximal duplicated genes associated with general fruit and seed traits and defense mechanisms, supporting the hypothesis of potential episodes of subfunctionalization and neofunctionalization following duplication, as well as impact from distinct domestication process. These genomic variations may underpin the differences observed in fruit and seed morphology, ripening, and disease resistance between cupuassu and the other Malvaceae species. Conclusions The cupuassu genome offers a foundational resource for both breeding improvement and conservation biology, yielding insights into the evolution and diversity within the genus Theobroma.

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Yeast-derived potato patatins: Biochemical and biophysical characterization

Cited by 6 publications

References 47 publications

Long‐time high‐pressure processing of a patatin‐rich potato proteins isolate: impact on aggregation and surface properties

Long‐time high‐pressure processing of a patatin‐rich potato proteins isolate: impact on aggregation and surface properties

Genomic decoding ofTheobroma grandiflorum(cupuassu) at chromosomal scale: Evolutionary insights for horticultural innovation

Genomic decoding of Theobroma grandiflorum (cupuassu) at chromosomal scale: evolutionary insights for horticultural innovation

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