Yeast exoglycoproteins produced under NaCl‐stress conditions as efficient cryoprotectiveagents

Abstract

Six extracellular yeast glycoproteins were prepared from three yeast species in osmoticequilibrium and unequilibrium environments and used as non‐penetrating cryoadditives. Theglycoprotein secreted by the strain Dipodascus australiensis into the growth mediumcontaining NaCl (8% w/v) was found to be the most effective cryoadditive. It was possible to usethis glycoprotein alone (without penetrating agent DMSO) for the cryoprotection of the yeastsstudied.

Biological Activity of Pulcherrimin from the Meschnikowia pulcherrima Clade

“…The addition of a small amount of pulcherrimin to the standard DMSO medium improved the survival of yeast strains after storage at the temperature of liquid nitrogen. Pulcherrimin protected the yeast cells like another biological cryoprotectant, yeast exoglycoprotein, which has been used in the CCY [ 32 ].…”

“…The second series was prepared in the same way as the first series but was supplemented with a non-penetrating cryoprotective agent—the extracellular yeast glycoprotein, which was added to a final concentration of 0.27% ( w / v ). Glycoprotein was obtained using the method of development in CCY [ 32 ]. The third series was again prepared in the same way but was supplemented with the yeast pulcherrimin to a final concentration of 0.006% ( w / v ).…”