Yeast for Pentose Fermentation: Isolation, Screening, Performance, Manipulation, and Prospects

Ceccato-Antonini, Sandra Regina; Codato, Carolina Brito; Martini, Cristina; Bastos, Reinaldo Gaspar; Tauk-Tornisielo, Sâmia Maria

doi:10.1007/978-3-319-49826-3_8

Cited by 8 publications

(11 citation statements)

References 115 publications

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“…However, it is not able to ferment sugars such as xylose, arabinose and others. These sugars make up the substrate of interest in lignocellulosic biomass, so that research for yeasts able to ferment them is necessary to increase ethanol production (Rossi and Andrietta, 2009;Ceccato-Antonini et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Ethanol production from Dekkera bruxellensis in synthetic media with pentose

Codato

Martini

Ceccato-Antonini

et al. 2018

Braz. J. Chem. Eng.

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Ethanol is obtained in Brazil from the fermentation of sugarcane, molasses or a mixture of these. Alternatively, it can also be obtained from products composed of cellulose and hemicellulose, called "second generation ethanol-2G". The yeast Saccharomyces cerevisiae, commonly applied in industrial ethanol production, is not efficient in the conversion of pentoses, which is present in high amounts in lignocellulosic materials. This study aimed to evaluate the ability of a yeast strain of Dekkera bruxellensis in producing ethanol from synthetic media, containing xylose or arabinose, xylose and glucose as the sole carbon sources. The results indicated that D. bruxellensis was capable of producing ethanol from xylose and arabinose, with ethanol concentration similar for both carbon sources, 1.9 g L-1. For the fermentations performed with xylose and glucose, there was an increase in the concentration of ethanol to 5.9 g L-1 , lower than the standard yeast Pichia stipitis (9.3 g L-1), but with similar maximum yield in ethanol (0.9 g g TOC-1). This proves that the yeast D. bruxellensis produced lower amounts of ethanol when compared with P. stipitis, but showed that is capable of fermenting xylose and can be a promising alternative for ethanol conversion from hydrolysates containing glucose and xylose as carbon source.

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Section: Introductionmentioning

confidence: 99%

Ethanol production from Dekkera bruxellensis in synthetic media with pentose

Codato

Martini

Ceccato-Antonini

et al. 2018

Braz. J. Chem. Eng.

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“…Biotechnologically, the strains and species characterized here show interesting features that warrant further exploration. K. quercitrusa growth on xylose makes them interesting for industrial applications, especially if they are able to co-ferment xylose in the presence of hexoses ( Hahn-Hägerdal et al 2007 ; Ceccato-Antonini et al 2017 ). Especially, the characteristics of K. quercitrusa var.…”

Section: Discussionmentioning

confidence: 99%

Isolation, characterization, and genome assembly of Barnettozyma botsteinii sp. nov. and novel strains of Kurtzmaniella quercitrusa isolated from the intestinal tract of the termite Macrotermes bellicosus

Arrey

Murphy

et al. 2021

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Bioconversion of hemicelluloses into simpler sugars leads to production of a significant amount of pentose sugars, such as D-xylose. However, efficient utilization of pentoses by conventional yeast production strains remains challenging. Wild yeast strains can provide new industrially relevant characteristics and efficiently utilize pentose sugars. To explore this strategy, we isolated gut-residing yeasts from the termite Macrotermes bellicosus collected in Comoé National Park, Côte d´Ivoire. The yeasts were classified through their ITS/LSU sequence, their genomes were sequenced and annotated. We identified a novel yeast species, which we name Barnettozyma botsteinii sp. nov. 1118T (MycoBank: 833563, CBS 16679T and IBT 710) and two new strains of Kurtzmaniella quercitrusa: var. comoensis (CBS 16678, IBT 709) and var. filamentosus (CBS 16680, IBT 711). The two K. quercitrusa strains grow 15% faster on synthetic glucose medium than Saccharomyces cerevisiae CEN.PKT in acidic conditions (pH = 3.2) and both strains grow on D-xylose as the sole carbon source at a rate of 0.35 h−1. At neutral pH, the yeast form of K. quercitrusa var. filamentosus, but not var. comoensis, switched to filamentous growth in a carbon source dependent manner. Their genomes are 11.0-13.2 Mb in size and contain between 4888 and 5475 predicted genes. Together with closely related species, we did not find any relationship between gene content and ability to grow on xylose. Besides its metabolism, K. quercitrusa var. filamentosus also has a large potential as a production organism, because of its capacity to grow at low pH and to undergo a dimorphic shift.

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“…But the main problem in bioethanol production from lignocellulose is that S. cerevisiae is not able to assimilate cellulose and hemicellulose directly. A way to overcome this obstacle is the use of pentose fermenting microorganisms like yeast such as Pichia stipitis, Candida shehatae and P. tannophilus which can ferment pentoses but their ethanol production rate from glucose is five times less than that observe for S. cerevisiae (Ceccato-Antonini et al, 2017). T. versicolor has also been reported to produce ethanol from xylose under aerobic conditions and similar property has also been found in zygomycete strains (Millati et al, 2005).…”

Section: Issn: 2319-7706 Volume 7 Number 01 (2018)mentioning

confidence: 99%