Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing

Sharp, Judith A.; Fouts, Erik T.; Krawitz, Denise C.; Kaufman, Paul D.

doi:10.1016/s0960-9822(01)00140-3

Cited by 250 publications

(382 citation statements)

References 53 publications

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“…Yeast Hir1p and Hir2p share several biological and biochemical properties with Asf1p. Like Asf1p, they are required for heterochromatin-mediated silencing of telomeres and mating loci, and are also required for formation of proper pericentromeric chromatin (but in an Asf1p-independent manner) (64,67,116,117). Mouse ES cells lacking HIRA have a larger pool of loosely bound histones than wild type cells, consistent with a role for HIRA in generation of compact, nucleosome-dense, transcriptionally silent heterochromatin (78).…”

Section: Chromosome Condensation Is Driven By Histone Chaperones Hiramentioning

confidence: 66%

“…In vitro, HIRA has been shown to act as a histone chaperone, preferentially depositing the histone variant histone H3.3 into nucleosomes, compared to canonical histone H3.1 (49,72,100,102,125). Consistent with their partially overlapping functions, Asf1 and Hir proteins physically interact and in yeast this interaction is necessary for telomeric silencing (32,49,116).…”

Section: Chromosome Condensation Is Driven By Histone Chaperones Hiramentioning

confidence: 98%

“…HIRA and ASF1a are the human orthologs of proteins known to create transcriptionally silent heterochromatin in yeast, flies and plants (64,83,97,117,121). Yeast Asf1p is required for heterochromatin-mediated silencing of telomeres and mating loci and has histone deposition activity in vitro (67,116,121,127). Yeast Asf1p is also required for histone eviction and subsequent replacement at transcribed genes (1,66,111), and also plays a role in DNA replication-coupled chromatin assembly (44,51,86,110,127).…”

Section: Chromosome Condensation Is Driven By Histone Chaperones Hiramentioning

confidence: 99%

See 2 more Smart Citations

Remodeling of chromatin structure in senescent cells and its potential impact on tumor suppression and aging

Adams

2007

Gene

162

135

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Cellular senescence is an important tumor suppression process, and a possible contributor to tissue aging. Senescence is accompanied extensive changes in chromatin structure. In particular, many senescent cells accumulate specialized domains of facultative heterochromatin, called Senescence Associated Heterochromatin Foci (SAHF), which are thought to repress expression of proliferationpromoting genes, thereby contributing to senescence-associated proliferation arrest. This article reviews our current understanding of the structure, assembly and function of these SAHF at a cellular level. The possible contribution of SAHF to tumor suppression and tissue aging is also critically discussed.

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Section: Chromosome Condensation Is Driven By Histone Chaperones Hiramentioning

confidence: 66%

Section: Chromosome Condensation Is Driven By Histone Chaperones Hiramentioning

confidence: 98%

Section: Chromosome Condensation Is Driven By Histone Chaperones Hiramentioning

confidence: 99%

See 1 more Smart Citation

Remodeling of chromatin structure in senescent cells and its potential impact on tumor suppression and aging

Adams

2007

Gene

162

135

View full text Add to dashboard Cite

show abstract

“…Significantly, at least one of these, the histone chaperone HIRA, has been shown to increase in expression or undergo some level of regulation in aging baboon skin [40,41]. Although the HIRA histone chaperone may have some functions linked to transcription activation [42][43][44][45][46][47][48], this chaperone is known to have an evolutionarily conserved role in heterochromatin formation [49][50][51][52][53][54][55][56].…”

Section: Aging Is Associated With Epigenetic Changes From Yeast To Hmentioning

confidence: 99%

Aging by epigenetics—A consequence of chromatin damage?

Sedivy

Gowrishankar

Adams

2008

Experimental Cell Research

142

102

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Chromatin structure is not fixed. Instead, chromatin is dynamic and is subject to extensive developmental and age-associated remodeling. In some cases, this remodeling appears to counter the aging and age-associated diseases, such as cancer, and extend organismal lifespan. However, stochastic non-deterministic changes in chromatin structure might, over time, also contribute to the break down of nuclear, cell and tissue function, and consequently aging and age-associated diseases.

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“…In S. cerevisiae, Hir gene products (HIR1 and HIR2) are implicated in a nucleosome assembly pathway that functionally overlaps that of chromatin assembly factor-1 (CAF-I) (Sharp et al 2001). HIR1 and HIR2 have been shown to be important for normal growth and gene silencing in the absence of CAF-I (Kaufman et al 1998;Qian et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Identical sequences but different expression patterns of Hira gene in gynogenetic and gonochoristic crucian carps

Zhou

Zhao

et al. 2007

Fish Physiol Biochem

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Hir/Hira (histone regulation) genes were first identified in yeast as negative regulators of histone gene expression. It has been confirmed that HIRA is a conserved family of proteins present in various animals and plants. In this paper, the cDNAs of the Hira homolog named CagHira and CaHira were isolated from gynogenetic gibel carp (gynocarp) and gonochoristic color crucian carp (gonocarp) respectively. The full-length CagHira is 3,860 bp in length with an open reading frame (ORF) of 3,033 bp that encodes 1,011 amino acids, while the full-length CaHira is 3,748 bp in length and also has an ORF of 3,033 bp. The deduced amino acid sequences of both Hira homologs contain seven WD domains and show high identity with other HIRA family members. RT-PCR analyses revealed strong expression of Hira in the ovaries, whereas no expression was detected in the testes of either of the fishes. Hira transcription was not detected in the liver of gyno-carp, but a high level of Hira mRNA was observed in gono-carp. The temporal expression pattern showed that the Hira mRNA is consistently expressed during all embryonic development stages in gyno-carp. However, the abundance of CaHira mRNA significantly decreased (P < 0.05) shortly after fertilization and then increased again and remained stable from gastrula till hatching. The varying spatiotemporal expression patterns of Hira genes in gyno-carp and gono-carp may be associated with the differing reproductive modes used by these two closely related fishes. Our results suggest that Hira may play a role not only in the decondensation of sperm nucleus and the formation of pronucleus during fertilization, but also in gastrulation and the subsequent development of embryos.

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Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing

Abstract: Yeast CAF-I and Asf1p cooperate to form nucleosomes in vitro. In vivo, Asf1p and Hir proteins physically interact and together promote heterochromatic gene silencing in a manner requiring PCNA. This Asf1/Hir silencing pathway functionally overlaps with CAF-I activity.

Cited by 250 publications

References 53 publications

Remodeling of chromatin structure in senescent cells and its potential impact on tumor suppression and aging

Remodeling of chromatin structure in senescent cells and its potential impact on tumor suppression and aging

Aging by epigenetics—A consequence of chromatin damage?

Identical sequences but different expression patterns of Hira gene in gynogenetic and gonochoristic crucian carps

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