Yeast Pyruvate Kinase

Yeast pyruvate kinase. Native and subunit molecular weight

“…Preparation of Enzyme. Pyruvate kinase was isolated from fresh "Budweiser" Baker's yeast (Anheuser-Busch, Inc.) according to the procedure of Hunsley and Suelter (1969a) and stock enzyme was stored as a suspension in 90% saturated (3.6 m) (NH4)>S04. Protein concentrations were estimated from the absorbance at 280 nm (Eflf 0.653) (Hunsley and Suelter, 1969a).…”

“…Some physical characteristics of yeast, rabbit muscle, and rat liver pyruvate kinases are compared. and Suelter (1969b). All enzyme preparations had a minimum specific activity of 210 /¿moles/min per mg at 30 °.…”

Analysis of Hill slopes predicted by the four-ligand exponential model for a regulatory enzyme

“…It is obvious that the limit is broken because the dependence of the velocity on the concentration of substrates does not follow the simple model on which the Hill equation is based. It can also be noted that Hill slopes greater than 4 have been measured in kinetic studies of pyruvate kinase by Hunsley & Suelter (1969). (9) Shape of Hill plot Artificial data have been constructed according to eqns.…”

Untitled

“…The investigation reported here was carried out in order to assess the state of the pyruvate kinase reaction during glyconeogenesis from ethanol by nongrowing cells of Saccharomyces cerevisiae. The results indicate that conversion of PEP to pyruvate was controlled at a low level, due to a low concentration of the allosteric activator FDP and substrate PEP [2,3]. The yeast, a laboratory strain of Saccharomyces cerevisiae, was grown in a mineral salts medium [4] containing 0.25% Difco yeast extract and 1% ethanol and harvested in the logarithmic phase of growth.…”