Yeast ribosomal protein L26 is located at the ribosomal subunit interface as determined by chemical cross-linking

Villarreal, Jr J; Lee, JC

doi:10.1016/s0300-9084(98)80074-6

Cited by 9 publications

(3 citation statements)

References 13 publications

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“…Our study also revealed that the ribosomal protein L26 (rpl26) was upregulated in the HK of fish fed the phytogenicsupplemented diet, which is involved in the abovementioned "signal transduction by p53 class mediator" biological process. This gene is located at the ribosomal subunit interface of the 60S subunit inside the cell (94). Interestingly, several studies have demonstrated the role of the rpl26 gene as a phagocytosisactivating protein, thus being highly involved in the immune response, since phagocytosis is a major mechanism used to remove pathogens and cell debris (95)(96)(97).…”

Section: Inflammatory Response and Immune Signalingmentioning

confidence: 99%

A Bioactive Extract Rich in Triterpenic Acid and Polyphenols from Olea europaea Promotes Systemic Immunity and Protects Atlantic Salmon Smolts Against Furunculosis

et al. 2021

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In the present study, the modulation of the transcriptional immune response (microarray analysis) in the head kidney (HK) of the anadromous fish Atlantic salmon (Salmo salar) fed a diet supplemented with an olive fruit extract (AQUOLIVE®) was evaluated. At the end of the trial (133 days), in order to investigate the immunomodulatory properties of the phytogenic tested against a bacterial infection, an in vivo challenge with Aeromonas salmonicida was performed. A total number of 1,027 differentially expressed genes (DEGs) (805 up- and 222 downregulated) were found when comparing the transcriptomic profiling of the HK from fish fed the control and AQUOLIVE® diets. The HK transcripteractome revealed an expression profile that mainly favored biological processes related to immunity. Particularly, the signaling of i-kappa B kinase/NF-kappa and the activation of leukocytes, such as granulocytes and neutrophils degranulation, were suggested to be the primary actors of the innate immune response promoted by the tested functional feed additive in the HK. Moreover, the bacterial challenge with A. salmonicida that lasted 12 days showed that the cumulative survival was higher in fish fed the AQUOLIVE® diet (96.9 ± 6.4%) than the control group (60.7 ± 13.5%). These results indicate that the dietary supplementation of AQUOLIVE® at the level of 0.15% enhanced the systemic immune response and reduced the A. salmonicida cumulative mortality in Atlantic salmon smolts.

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Section: Inflammatory Response and Immune Signalingmentioning

confidence: 99%

A Bioactive Extract Rich in Triterpenic Acid and Polyphenols from Olea europaea Promotes Systemic Immunity and Protects Atlantic Salmon Smolts Against Furunculosis

et al. 2021

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“…Transcript levels of L26 steadily increased over the course of anoxia exposure, via active transcription, and remained high following aerobic recovery. Ribosomal protein L26 is located at the interface of the large and small subunit, specifically at the peptidyl transferase center [59, 60] and has been shown to interact with elongation factor-2 (EF-2) [61]. Hence, L26 is proposed to be involved in subunit interactions, perhaps as a stabilizing factor, based on the observation that it undergoes structural rearrangement as ribosomal subunits associate [62].…”

Section: The Functional Responsementioning

confidence: 99%

Living without Oxygen: Anoxia-Responsive Gene Expression and Regulation

Larade¹,

Storey

2009

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Many species of marine mollusks demonstrate exceptional capacities for long term survival without oxygen. Analysis of gene expression under anoxic conditions, including the subsequent translational responses, allows examination of the functional mechanisms that support and regulate natural anaerobiosis and permit noninjurious transitions between aerobic and anoxic states. Identification of stress-specific gene expression can provide important insights into the metabolic adaptations that are needed for anoxia tolerance, with potential applications to anoxia-intolerant systems. Various methods are available to do this, including high throughput microarray screening and construction and screening of cDNA libraries. Anoxia-responsive genes have been identified in mollusks; some have known functions in other organisms but were not previously linked with anoxia survival. In other cases, completely novel anoxia-responsive genes have been discovered, some that show known motifs or domains that hint at function. Selected genes are expressed at different times over an anoxia-recovery time course with their transcription and translation being actively regulated to ensure protein expression at the optimal time. An examination of transcript status over the course of anoxia exposure and subsequent aerobic recovery identifies genes, and the proteins that they encode, that enhance cell survival under oxygen-limited conditions. Analysis of data generated from non-mainstream model systems allows for insight into the response by cells to anoxia stress.

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“…In contrast to RpS9, RpL26 has been little studied, but like rpS9, sequences are available for B. mori and D. melanogaster. Eukaryotic RpL26 is most closely related to bacterial RpL24, and in yeast, Villarreal and Lee (1998) have shown that the protein is located at the interface between the small and large ribosomal subunits. Larede et al (2001) identified RpL26 from a marine snail, and in this species, RpL26 is up-regulated during anoxia.…”

Section: Archives Of Insect Biochemistry and Physiologymentioning

confidence: 99%

Recovery of cDNAs encoding ribosomal proteins S9 and L26 fromAedes albopictus mosquito cells and identification of their homologs in the malaria vector,Anopheles gambiae

Fallon

2005

Arch. Insect Biochem. Physiol.

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We used PCR-based approaches to obtain the full-length cDNA sequences encoding ribosomal protein (Rp) S9 and L26 from a mosquito (Aedes albopictus) C7-10 cell line. The deduced mosquito RpS9 protein has a mass of 22,826 Da and a pI of 11.41, while RpL26 had a mass of 17,442 Da and a pI of 11.52. Both cDNAs initiated with the 5'-polypyrimidine motif characteristic of ribosomal protein transcripts. Using the Aedes protein and nucleic acid sequences, we identified rpS9 and rpL26 as single copy genes in the Anopheles gambiae genome. In An. gambiae, the RpS9 coding region was distributed over 3 exons, spanning 2.6 kb, but the Anopheles rpL26 protein coding region lacked introns. The Aedes and Anopheles RpS9 and RpL26 proteins shared 96 and 92% identity, respectively. Despite low numbers of parsimony-informative amino acid substitutions, phylogenies based on the ribosomal protein sequences accurately group the Aedes and Anopheles proteins with high bootstrap values.

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Yeast ribosomal protein L26 is located at the ribosomal subunit interface as determined by chemical cross-linking

Cited by 9 publications

References 13 publications

A Bioactive Extract Rich in Triterpenic Acid and Polyphenols from Olea europaea Promotes Systemic Immunity and Protects Atlantic Salmon Smolts Against Furunculosis

A Bioactive Extract Rich in Triterpenic Acid and Polyphenols from Olea europaea Promotes Systemic Immunity and Protects Atlantic Salmon Smolts Against Furunculosis

Living without Oxygen: Anoxia-Responsive Gene Expression and Regulation

Recovery of cDNAs encoding ribosomal proteins S9 and L26 fromAedes albopictus mosquito cells and identification of their homologs in the malaria vector,Anopheles gambiae

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