Yeast Surface Display of <i>Antheraea pernyi</i> Lysozyme Revealed α-Helical Antibacterial Peptides in Its N-Terminal Domain

Wen, Sai; Mao, Tong-xin; Yao, Dongmei; Tian, Li; Wang, Fenghuan

doi:10.1021/acs.jafc.8b02489

Cited by 7 publications

(4 citation statements)

References 65 publications

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“…A new type of lysozyme from the Chinese oak silk moth ( Antheraea pernyi ) was investigated and the encouraging results obtained about the strong effectiveness against Gram-negative strains, according to the authors, laid the foundation for future improvement by protein engineering [ 64 ].…”

Section: Sourcesmentioning

confidence: 99%

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Ferraboschi

Ciceri

Grisenti³

2021

Antibiotics

209

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Lysozyme is a ~14 kDa protein present in many mucosal secretions (tears, saliva, and mucus) and tissues of animals and plants, and plays an important role in the innate immunity, providing protection against bacteria, viruses, and fungi. Three main different types of lysozymes are known: the c-type (chicken or conventional type), the g-type (goose type), and the i-type (invertebrate type). It has long been the subject of several applications due to its antimicrobial properties. The problem of antibiotic resistance has stimulated the search for new molecules or new applications of known compounds. The use of lysozyme as an alternative antibiotic is the subject of this review, which covers the results published over the past two decades. This review is focused on the applications of lysozyme in medicine, (the treatment of infectious diseases, wound healing, and anti-biofilm), veterinary, feed, food preservation, and crop protection. It is available from a wide range of sources, in addition to the well-known chicken egg white, and its synergism with other compounds, endowed with antimicrobial activity, are also summarized. An overview of the modified lysozyme applications is provided in the form of tables.

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Section: Sourcesmentioning

confidence: 99%

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Ferraboschi

Ciceri

Grisenti³

2021

Antibiotics

209

View full text Add to dashboard Cite

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“…ATCC 10988 (GenBank: AAA27695.1) [25]. The mode of fusion has an important effect on the function of the fusion protein, depending mainly on the structure of the protein [26]. To ensure the activity of levansucrase, two yeast display systems, a-agglutinin and Flo1p, were chosen to display levansucrase on the cell surface of S. cerevisiae.…”

Section: Construction Of Two Yeast Cell Surface Display Systemsmentioning

confidence: 99%

Development and Characterization of Two Types of Surface Displayed Levansucrases for Levan Biosynthesis

et al. 2021

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Levan has wide applications in chemical, cosmetic, pharmaceutical and food industries. The free levansucrase is usually used in the biosynthesis of levan, but the poor reusability and low stability of free levansucrase have limited its large-scale use. To address this problem, the surface-displayed levansucrase in Saccharomyces cerevisiae were generated and evaluated in this study. The levansucrase from Zymomonas mobilis was displayed on the cell surface of Saccharomyces cerevisiae EBY100 using a various yeast surface display platform. The N-terminal fusion partner is based on a-agglutinin, and the C-terminal one is Flo1p. The yield of levan produced by these two whole-cell biocatalysts reaches 26 g/L and 34 g/L in 24 h, respectively. Meanwhile, the stability of the surface-displayed levansucrases is significantly enhanced. After six reuses, these two biocatalysts retained over 50% and 60% of their initial activities, respectively. Furthermore, the molecular weight and polydispersity test of the products suggested that the whole-cell biocatalyst of levansucrase displayed by Flo1p has more potentials in the production of levan with low molecular weight which is critical in certain applications. In conclusion, our method not only enable the possibility to reuse the enzyme, but also improves the stability of the enzyme.

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“…Use of a yeast surface display platform to anchor recombinant proteins to yeast cell wall has been widely adapted in the biomedical field for antibody and antimicrobial peptide engineering, protein stability, protein–protein interaction, and enzyme metabolic studies ( Cherf and Cochran, 2015 ; Wen et al, 2018 ; Medina-Cucurella et al, 2019 ; Lau et al, 2021 ; Luo et al, 2021 ; Fiebig et al, 2022 ; Kuroda and Ueda, 2022 ; Raeeszadeh-Sarmazdeh and Boder, 2022 ; Teymennet-Ramirez et al, 2022 ). A common yeast display system involves fusing recombinant proteins to the a-agglutinin mating protein Aga2p and expressing extracellularly in yeast that secrete the ß 1,6 glucan-anchored Aga1p domain of a-agglutinin to form an Aga1p–Aga2p complex via two disulfide bonds ( Wang et al, 2005 ; Cherf and Cochran, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Saccharomyces cerevisiae surface display of endolysin LysKB317 for control of bacterial contamination in corn ethanol fermentations

Liu

Patel

et al. 2023

Front. Bioeng. Biotechnol.

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Control of bacterial contamination in bioethanol fermentation facilities has traditionally relied on chemical-based products such as hop acids and use of antibiotics. Recent emphasis on antibiotic stewardship has prompted new research into the development of alternative approaches to microbial remediation strategies. We recently described a recombinant peptidoglycan hydrolase, endolysin LysKB317, which inhibited Limosilactobacillus fermentum strains in corn mash fermentation. Here, Saccharomyces cerevisiae EBY100 was used to anchor recombinant LysKB317 using cell surface display with the a-agglutinin proteins Aga1p–Aga2p. Immunostaining and confocal fluorescence were used for localization of the extracellular interface of the cells. Yeast surface-expressed endolysin demonstrated an 83.8% decrease in bacterial cell counts compared to a 9.5% decrease in control yeast. Recombinant S. cerevisiae expressing LysKB317 used for small-scale corn mash fermentation, when infected with L. fermentum, could proactively control bacterial infection for 72 h with at least 1-log fold reduction. Analysis of fermentation products showed improved ethanol concentrations from 3.4% to at least 5.9% compared to the infection-only control and reduced levels of lactic and acetic acid from 34.7 mM to 13.8 mM and 25.5 mM to 18.1 mM, respectively. In an optimized yeast surface display system, proactive treatment of bacterial contaminants by endolysin LysKB317 can improve fermentation efficiency in the presence of L. fermentum contamination.

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Yeast Surface Display of Antheraea pernyi Lysozyme Revealed α-Helical Antibacterial Peptides in Its N-Terminal Domain

Cited by 7 publications

References 65 publications

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Development and Characterization of Two Types of Surface Displayed Levansucrases for Levan Biosynthesis

Saccharomyces cerevisiae surface display of endolysin LysKB317 for control of bacterial contamination in corn ethanol fermentations

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