Yeast Synthetic Biology for Production of Artemisinin as an Antimalarial Drug

Kashkooli, Arman Beyraghdar; Farmanpour-Kalalagh, Karim; Babaei, Alireza

doi:10.1007/978-3-030-89680-5_6

Cited by 4 publications

(2 citation statements)

References 139 publications

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“…For meeting large-scale demands, the de novo reconstitution of artemisinin biosynthesis in heterologous microbes, including S. cerevisiae and E. coli, has noteworthy achievements, for instance, engineering the carbon flux and genotype of S. cerevisiae yields high quantities of artemisinic acid. Moreover, S. cerevisiae has also demonstrated itself as a heterologous host for producing artemisinin precursors, such as artemisinic acid, dihydro-artemisinic acid, and amorphadiene [122].…”

Section: Plant Natural Productsmentioning

confidence: 99%

Microbial Cell Factories: Biodiversity, Pathway Construction, Robustness, and Industrial Applicability

Chaudhary,

Nawaz,

Fouillaud

et al. 2024

Microbiology Research

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The microbial biosynthesis of proteins, primary metabolites, and chemicals is gaining extraordinary momentum and is presently viewed as an advancing approach in the industrial research sector. Increased threats to the environment and the possibility of declining petroleum assets have switched the spotlight to microbial cell factories (MCFs). Aside from possessing various advantages over chemical synthesis, such as less toxicity, cheaper methodologies, and an environmentally benign nature, microbes can be cultivated in fermenters, resulting in an effective bioprocessing approach in terms of industrial relevance. As the overwhelming majority of biodiversity is microbial, this review first highlights the microbial biodiversity of industrially vital microorganisms. Then, the paper delineates the production pathways for generating valuable bioproducts via microbial workhorses. Many host cells synthesize bio-compounds as a part of their natural mechanism; however, several techniques have also been developed to attain the desired end product from non-native microbes with selected properties. The microbial biosynthetic pathways can be categorized as native-existing pathways, heterologous pathways, and artificial de novo pathways. Systems metabolic engineering, which integrates metabolic engineering with evolutionary engineering, synthetic biology, and systems biology, has further revolutionized the field of engineering robust phenotypes. The employment of these strategies improves the performance of the strain, eventually achieving high titer and productivity rates of bio-chemicals. Modern trends and tools for exploiting native pathways and designing non-native-created pathways are also briefly discussed in this paper. Finally, the review discusses the use of microbial workhorses for producing a myriad of materials and chemicals, including carboxylic acids, amino acids, plant natural products (PNPs), carotenoids, flavors, and fragrances, unveiling the efficacy of utilizing microbial species to generate sustainable bio-based products.

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Section: Plant Natural Productsmentioning

confidence: 99%

Microbial Cell Factories: Biodiversity, Pathway Construction, Robustness, and Industrial Applicability

Chaudhary,

Nawaz,

Fouillaud

et al. 2024

Microbiology Research

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“…For cluster analysis, NTSYS software version 2.2 was used to group ecotypes separately by three DTs. A discriminant function analysis (DFA) test was also performed to ensure accurate clustering of the initial dendrogram according to Nikrouz-Gharamaleki et al (2019).…”

Section: Univariate and Multivariate Analysesmentioning

confidence: 99%

Drying temperatures affect the qualitative–quantitative variation of aromatic profiling in Anethum graveolens L. ecotypes as an industrial–medicinal–vegetable plant

Farmanpour Kalalagh,

Mohebodini,

Fattahi

et al. 2023

Front. Plant Sci.

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IntroductionThere are several factors that affect the quality and quantity of active ingredients and essential oil (EO) content, including pre and postharvest practices such as drying conditions. One of the most important factors in drying is temperature and then selective drying temperature (DT). In general, DT has a direct effect on the aromatic properties of Anethum graveolens.MethodsOn this basis, the present study was conducted to evaluate the effects of different DTs on the aroma profile of A. graveolens ecotypes.Results and discussionThe results showed that different DTs, ecotypes, and their interaction significantly affect EO content and composition. The highest EO yield was obtained from the Parsabad ecotype (1.86%) followed by the Ardabil ecotype (1.4%), both at 40° C. More than 60 EO compounds were identified, mainly monoterpenes and sesquiterpenes, highlighting α-Phellandrene, Germacrene D, and Dill apiole as major components in all treatments. Besides α-Phellandrene, the major EO compounds at shad drying (ShD) were β-Phellandrene and p-Cymene, while plant parts dried at 40° C showed l-Limonene and Limonene as the main constituents, and Dill apiole was detected in greater amounts in the samples dried at 60 °C. To determine the appropriate DT, simple and factorial based-ANOVA together multivariate analysis demonstrated significant differences in the compounds produced under different DTs. The results indicated that more EO compounds, mainly monoterpenes, were extracted at ShD than other DTs. On the other hand, the content and composition of sesquiterpenes increased significantly when DT was increased to 60 °C. From the genetic backgrounds point of view, the Parsabad ecotype (with 12 similar compounds) and Esfahan ecotype (with 10 similar compounds) were the most suitable ecotypes under all DTs in terms of EO compounds. Accordingly, the present study would help various industries to optimize specific DT(s) to obtain special EO compound(s) from different A. graveolens ecotypes based on commercial requirements.

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HIV and the Role of Various Medicinal Plants Against Infection in Humans, with Possible Mechanism of Action and Functions of Secondary Metabolites

Malik

Pal

2022

Reference Series in Phytochemistry

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Yeast Synthetic Biology for Production of Artemisinin as an Antimalarial Drug

Cited by 4 publications

References 139 publications

Microbial Cell Factories: Biodiversity, Pathway Construction, Robustness, and Industrial Applicability

Microbial Cell Factories: Biodiversity, Pathway Construction, Robustness, and Industrial Applicability

Drying temperatures affect the qualitative–quantitative variation of aromatic profiling in Anethum graveolens L. ecotypes as an industrial–medicinal–vegetable plant

HIV and the Role of Various Medicinal Plants Against Infection in Humans, with Possible Mechanism of Action and Functions of Secondary Metabolites

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