“…Although many software tools dedicated to the analysis of budding yeast live-cell microscopy have been developed in the past (ImageJ/Fiji 10 , MorphoLibJ 11 , PhyloCell 12 , CellProfiler 13 , Cell Tracer 14 , Wood et al 15,16 , Cell Star 17 , Cell Serpent 18 , CellID 19 , Tracker 20 , DISCO 21 , YeastSpotter 22 , YeastNet 23 , DeepCell 24 , Cellbow 25 ), to the best of our knowledge, none of them spanned the entire image analysis pipeline from CNN-base segmentation to cell cycle analysis, and fluorescent signal quantification.…”