Yeasts from Tierra Del Fuego Province (Argentina): Biodiversity, Characterization and Bioprospection of Hydrolytic Enzymes

Cavello, Ivana Alejandra; Bezus, Brenda; Martinez, A Rodriguez; Garmendia, Gabriela; Vero, Silvana; Cavalitto, Sebastián Fernando

doi:10.1080/01490451.2019.1641769

Cited by 13 publications

(18 citation statements)

References 59 publications

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“…In fact, its ability of producing darkly pigmented spores with a rigid wall may be consistent with its presence in ice cores older than 140,000 years (Ma et al ., 2000) or in some arid regions where the lack of rainfalls is combined with high salinity of soils (Kis‐Papo et al ., 2003; Sterflinger et al ., 2012). Likewise, the basidiomycetous genus Naganishia was one of the dominant genera found in fungal communities of some Antarctic brines, thus confirming its moderately halotolerant nature (Borruso et al ., 2018; Cavello et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

Intra‐ and inter‐cores fungal diversity suggests interconnection of different habitats in an Antarctic frozen lake (Boulder Clay, Northern Victoria Land)

Sannino

Borruso

Mezzasoma

et al. 2020

Environmental Microbiology

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Summary A perennially frozen lake at Boulder Clay site (Victoria Land, Antarctica), characterized by the presence of frost mounds, have been selected as an in situ model for ecological studies. Different samples of permafrost, glacier ice and brines have been studied as a unique habitat system. An additional sample of brines (collected in another frozen lake close to the previous one) was also considered. Alpha‐ and beta‐diversity of fungal communities showed both intra‐ and inter‐cores significant (p < 0.05) differences, which suggest the presence of interconnection among the habitats. Therefore, the layers of frost mound and the deep glacier could be interconnected while the brines could probably be considered as an open habitat system not interconnected with each other. Moreover, the absence of similarity between the lake ice and the underlying permafrost suggested that the lake is perennially frozen based. The predominance of positive significant (p < 0.05) co‐occurrences among some fungal taxa allowed to postulate the existence of an ecological equilibrium in the habitats systems. The positive significant (p < 0.05) correlation between salt concentration, total organic carbon and pH, and some fungal taxa suggests that a few abiotic parameters could drive fungal diversity inside these ecological niches.

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Section: Discussionmentioning

confidence: 99%

Intra‐ and inter‐cores fungal diversity suggests interconnection of different habitats in an Antarctic frozen lake (Boulder Clay, Northern Victoria Land)

Sannino

Borruso

Mezzasoma

et al. 2020

Environmental Microbiology

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“…At present, there are few studies on Tausonia . Tausonia can grow in the presence of high concentrations of NaCl, and we suspect that the significant increase in its relative abundance increases the number of salt-tolerant microorganisms in the soil [ 64 ]. Tausonia also has a variety of extracellular hydrolase activities, which can dissolve the cell walls of pathogenic fungi [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Salt-Tolerant Antagonistic Bacteria CZ-6 on the Rhizosphere Microbial Community of Winter Jujube (Ziziphus jujuba Mill. “Dongzao”) in Saline-Alkali Land

Zhou

Hao

et al. 2021

BioMed Research International

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As the main economic crop cultivated in the Yellow River Delta, winter jujube contains various nutrients. However, soil salinization and fungal diseases have affected the yield and quality of winter jujube. In order to use plant growth-promoting rhizobacteria (PGPR) to reduce these damages, the antagonistic bacteria CZ-6 isolated from the rhizosphere of wheat in saline soil was selected for experiment. Gene sequencing analysis identified CZ-6 as Bacillus amyloliquefaciens. In order to understand the salt tolerant and disease-resistant effects of CZ-6 strain, determination of related indicators of salt tolerance, pathogen antagonistic tests, and anti-fungal mechanism analyses was carried out. A pot experiment was conducted to evaluate the effect of CZ-6 inoculation on the rhizosphere microbial community of winter jujube. The salt tolerance test showed that CZ-6 strain can survive in a medium with a NaCl concentration of 10% and produces indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Studies on the inhibition mechanism of pathogenic fungi show that CZ-6 can secrete cellulase, protease, and xylanase. Gas chromatography-mass spectrometry (GC-MS) analysis showed that CZ-6 can release volatile organic compounds (VOCs), including 2-heptanone and 2-nonanone. In addition, the strain can colonize the rhizosphere and migrate to the roots, stems, and leaves of winter jujube, which is essential for plant growth or defense against pathogens. Illumina MiSeq sequencing data indicated that, compared to the control, the abundance of salt-tolerant bacteria Tausonia in the CZ-6 strain treatment group was significantly increased, while the richness of Chaetomium and Gibberella pathogens was significantly reduced. Our research shows that CZ-6 has the potential as a biological control agent in saline soil. Plant damage and economic losses caused by pathogenic fungi and salt stress are expected to be alleviated by the addition of salt-tolerant antagonistic bacteria.

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“…A sequence analysis of the D1/D2 variable domains at the 5´ end of the large rRNA subunit gene was carried out to identify the yeast isolates. DNA was extracted as described by Cavello et al (2019). In the polymerase chain reaction (PCR) process, the primers used - ITS1 (5´-TCC GTA GGT GAA CCT TGC GG-3´) and D2R (5´-GGT CCG TGT TTC AAG ACG-3´) - covered both the ITS1-ITS2 and the D1/D2 regions.…”

Section: Methodsmentioning

confidence: 99%

“…Few reports dealing with the search for new enzymes of industrial interest produced by cold-adapted bacteria, yeasts and filamentous fungi have been published (Lo Giudice & Fani 2015, Carrasco et al 2016, Martinez et al 2016, Martorell et al 2019). Enzymes are widely used in industrial processes, such as amylases in the detergent field, biopulping and environmental bioremediation, cellulases in the textile and detergent industries, pectinases in juice, wine-making and the paper industry, proteases in the detergent industry, lipases in the detergent field, inulinases for producing fructose and fructooligosaccharides and β-glucosidases in the wine-making industry (Adapa et al 2014, Białkowska & Turkiewicz 2014, de Ovalle et al 2018, Cavello et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Yeasts from the Maritime Antarctic: tools for industry and bioremediation

et al. 2021

Self Cite

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We isolated 32 yeasts from King George Island, which we then identified and characterized. Twenty-six belonged to Basidiomycota among the genera Naganishia, Holtermaniella, Vishniacozyma, Phenoliferia, Mrakia and Cystobasidium, and only six were Ascomycota of the genera Metschnikowia and Debaryomyces. Thirteen were psychrophiles, while 19 were psychrotolerant. Certain isolates exhibited a high tolerance to NaCl (3.5 M), while most tolerated Ni2+, Zn2+ and Li+. Cu2+ and Cd2+, however, inhibited the growth of most of the isolates. We assessed a bioprospecting of extracellular enzymes and their ability to biodegrade or bioaccumulate textile dyes. β-Glucosidases (59%) and esterases (53%) were the main extracellular enzymes detected. A minor proportion of the yeasts produced pectinases and xylanases; only psychrophiles produced proteases. Vishniacozyma, Naganishia, Phenoliferia and Mrakia were the richest genera in terms of enzyme production. Greater than 70% of the isolates decolourized solid medium supplemented with various dyes at 4°C and 20°C. Isolates belonging to the genera Vishniacozyma, Cystobasidium, Mrakia and Phenoliferia seem to have potential for textile dye bio-decolourization. The results demonstrated that yeasts collected from the Maritime Antarctic are a potential source of new enzymes of biotechnological interest, and that certain isolates could potentially be considered in the design of textile wastewater decolourizations.

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Yeasts from Tierra Del Fuego Province (Argentina): Biodiversity, Characterization and Bioprospection of Hydrolytic Enzymes

Cited by 13 publications

References 59 publications

Intra‐ and inter‐cores fungal diversity suggests interconnection of different habitats in an Antarctic frozen lake (Boulder Clay, Northern Victoria Land)

Intra‐ and inter‐cores fungal diversity suggests interconnection of different habitats in an Antarctic frozen lake (Boulder Clay, Northern Victoria Land)

The Effect of Salt-Tolerant Antagonistic Bacteria CZ-6 on the Rhizosphere Microbial Community of Winter Jujube (Ziziphus jujuba Mill. “Dongzao”) in Saline-Alkali Land

Yeasts from the Maritime Antarctic: tools for industry and bioremediation

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