Distilled alcoholic beverages are produced firstly by fermenting sugars emanating from cereal starches (in the case of whiskies), sucrose-rich plants (in the case of rums), fructooligosaccharide-rich plants (in the case of tequila) or from fruits (in the case of brandies). Traditionally, such fermentations were conducted in a spontaneous fashion, relying on indigenous microbiota, including wild yeasts. In modern practices, selected strains of Saccharomyces cerevisiae are employed to produce high levels of ethanol together with numerous secondary metabolites (eg. higher alcohols, esters, carbonyls etc.) which greatly influence the final flavour and aroma characteristics of spirits following distillation of the fermented wash. Therefore, distillers, like winemakers, must carefully choose their yeast strain which will be very important in providing the alcohol content and the sensory profiles of spirit beverages. This Chapter discusses yeast and fermentation aspects associated with the production of selected distilled spirits and highlights similarities and differences with the production of wine. Table of content: 16.1 Introduction 16.2 Yeasts in production of cereal-based spirits 16.3 Yeasts in the production of Agave and Dasylirion -based spirits 16.3.1 Distilled Agave and Dasylirion spirits: Tequila, mezcal, bacanora, raicilla and sotol 16.3.2 Tequila categories, types and production process 16.3.3 Mezcal, bacanora and sotol categories, types and production Processes 16.3.4 Yeast and fermentation aspects 16.3.5 Recent developments and future prospects 16.4 Yeasts in production of miscellaneous spirits 16.4.1 Yeasts in production of fruit-based spirits 16.4.2 Yeasts in production of sugarcane-based spirits 16.4.3 Yeasts in production of whey-based spirits 16.5 Conclusions & future prospects 16.6 References Process stage Beverage Agave used Volatile compounds References Raw material Tequila A. tequilana var. azul Terpenes, alcohols, furans, acids, aldehydes, esters, hydrocarbures Prado-Jaramillo et al. (2015) Mezcal, Tequila A. angustifolia, A. salmiana A. tequilana var. azul Acids, esters, terpenes Peña-Alvárez et al. (2006) Mezcal A. salmiana subsp. crassispina, A. salmiana var. salmiana, A. angustifolia, A. cupreata, A. karwinskii Acids, lipids Martínez-Aguilar et al (2009) Cooking/ hydrolysis Tequila A. tequilana var. azul Furans, pyrans, aldehydes, nitrogen and sulfur compounds terpenes, alcohols, furans, acids, aldehydes, esters, hydrocarbures Mancilla-Margalli and López (2002) Prado-Jaramillo et al. (2015) Mezcal A. salmianas Furans García-Soto et al. (2011) Fermentation Tequila A. tequilana var. azul Terpenes, alcohols, higher alcohols, furans, acids, esters, aldehydes, hydrocarbures